Genome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex

Abstract A rising incidence of meningococcal serogroup W disease has been evident in many countries worldwide. Serogroup W isolates belonging to the sequence type (ST)-11 clonal complex have been associated with atypical symptoms and increased case fatality rates. The continued expansion of this clo...

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Autores principales:	Bianca Stenmark, Lorraine Eriksson, Sara Thulin Hedberg, Brian P. Anton, Alexey Fomenkov, Richard J. Roberts, Paula Mölling
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/064fcb38cc664e919d2a01b417b66c1b
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spelling	oai:doaj.org-article:064fcb38cc664e919d2a01b417b66c1b2021-12-02T11:39:48ZGenome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex10.1038/s41598-021-85266-72045-2322https://doaj.org/article/064fcb38cc664e919d2a01b417b66c1b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85266-7https://doaj.org/toc/2045-2322Abstract A rising incidence of meningococcal serogroup W disease has been evident in many countries worldwide. Serogroup W isolates belonging to the sequence type (ST)-11 clonal complex have been associated with atypical symptoms and increased case fatality rates. The continued expansion of this clonal complex in the later part of the 2010s has been largely due to a shift from the so-called original UK strain to the 2013 strain. Here we used single-molecule real-time (SMRT) sequencing to determine the methylomes of the two major serogroup W strains belonging to ST-11 clonal complex. Five methylated motifs were identified in this study, and three of the motifs, namely 5′-GATC-3′, 5′-GAAGG-3′, 5′-GCGCGC-3′, were found in all 13 isolates investigated. The results showed no strain-specific motifs or difference in active restriction modification systems between the two strains. Two phase variable methylases were identified and the enrichment or depletion of the methylation motifs generated by these methylases varied between the two strains. Results from this work give further insight into the low diversity of methylomes in highly related strains and encourage further research to decipher the role of regions with under- or overrepresented methylation motifs.Bianca StenmarkLorraine ErikssonSara Thulin HedbergBrian P. AntonAlexey FomenkovRichard J. RobertsPaula MöllingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bianca Stenmark Lorraine Eriksson Sara Thulin Hedberg Brian P. Anton Alexey Fomenkov Richard J. Roberts Paula Mölling Genome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex
description	Abstract A rising incidence of meningococcal serogroup W disease has been evident in many countries worldwide. Serogroup W isolates belonging to the sequence type (ST)-11 clonal complex have been associated with atypical symptoms and increased case fatality rates. The continued expansion of this clonal complex in the later part of the 2010s has been largely due to a shift from the so-called original UK strain to the 2013 strain. Here we used single-molecule real-time (SMRT) sequencing to determine the methylomes of the two major serogroup W strains belonging to ST-11 clonal complex. Five methylated motifs were identified in this study, and three of the motifs, namely 5′-GATC-3′, 5′-GAAGG-3′, 5′-GCGCGC-3′, were found in all 13 isolates investigated. The results showed no strain-specific motifs or difference in active restriction modification systems between the two strains. Two phase variable methylases were identified and the enrichment or depletion of the methylation motifs generated by these methylases varied between the two strains. Results from this work give further insight into the low diversity of methylomes in highly related strains and encourage further research to decipher the role of regions with under- or overrepresented methylation motifs.
format	article
author	Bianca Stenmark Lorraine Eriksson Sara Thulin Hedberg Brian P. Anton Alexey Fomenkov Richard J. Roberts Paula Mölling
author_facet	Bianca Stenmark Lorraine Eriksson Sara Thulin Hedberg Brian P. Anton Alexey Fomenkov Richard J. Roberts Paula Mölling
author_sort	Bianca Stenmark
title	Genome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex
title_short	Genome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex
title_full	Genome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex
title_fullStr	Genome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex
title_full_unstemmed	Genome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex
title_sort	genome-wide methylome analysis of two strains belonging to the hypervirulent neisseria meningitidis serogroup w st-11 clonal complex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/064fcb38cc664e919d2a01b417b66c1b
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Genome-wide methylome analysis of two strains belonging to the hypervirulent Neisseria meningitidis serogroup W ST-11 clonal complex

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