Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen

Authors. 2018. Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen. Biodiversitas 19: 1186-1193. Tripa peat swamp forest is protected areas that have high biodiversity. Nevertheless, in some areas, the damage occurred due to conversions of la...

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Autores principales:	ZAIRIN THOMY, ARDHANA YULISMA, ESSY HARNELLY, ARIDA SUSILOWATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	tripa peat swamp forests identification phylogenetic relationships tree species 5.8s rrna nuclear gene Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/06591ae4cfeb40448401ea62df3ee288
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spelling	oai:doaj.org-article:06591ae4cfeb40448401ea62df3ee2882021-11-16T14:03:29ZMolecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen1412-033X2085-472210.13057/biodiv/d190402https://doaj.org/article/06591ae4cfeb40448401ea62df3ee2882018-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2766https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Authors. 2018. Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen. Biodiversitas 19: 1186-1193. Tripa peat swamp forest is protected areas that have high biodiversity. Nevertheless, in some areas, the damage occurred due to conversions of land function to oil palm plantations. The impact of conversions of peat swamp forest to oil palm plantations has led to biodiversity decreased. Hence, it is important to identify the remain tree species in Tripa peat swamp forest. This study aimed to determinetrees species diversity in Tripa peat swamp forest by using of 5.8S rRNA nuclear gene. Research was conducted at Forest Genetics and Molecular Forestry Laboratory, Faculty of Forestry, IPB from September 2015 to August 2016. Molecular identification consisted of DNA extraction, PCR analysis, and sequencing. The data were analyzed using Bioedit, MEGA 6, BLAST, and ITS2 database. Molecular identification using ITS 1 and ITS 4 primer successfully amplified (the ITS region ITS1-5.8S-ITS2) of 16 trees species from 9 families. BLAST analysis results indicate the presence of 16 species has similar bases sequence with the GeneBank DNA database. The plant species are Branckenridgea palustris (Ochnaceae), Gonystylus sp. (Thymelaeaceae), Tristaniopsis whiteana (Myrtaceae), Syzygium sp.1 (Myrtaceae), Macaranga triloba (Euphorbiaceae), Syzygium garciniifolium (Myrtaceae), Knema intermedia (Myristicaceae), Palaquium ridleyi (Sapotaceae), Palaquium sp. (Sapotaceae), Dyera lowii (Apocynaceae), Elaeocarpus petiolatus (Elaeocarpaceae), Ficus sp. (Moraceae), Syzygium leptostemon (Myrtaceae), Chilocarpus suaveolens (Apocynaceae), Alstonia pneumatophora (Apocynaceae), and Alstonia sp. (Apocynaceae). Phylogeny tree reconstruction using the Neighbor-Joining Method (NJ) showed that 5.8S rRNA nuclear gene was successful as marker for 16 trees species from 9 different families. In addition, the 5.8S also successful for resolving phylogenetic relationships at genus level i.e. Alstonia, Palaquium, Syzygium, Tristaniopsis, Macaranga, Elaeocarpus, and Ficus.ZAIRIN THOMYARDHANA YULISMAESSY HARNELLYARIDA SUSILOWATIMBI & UNS Soloarticletripa peat swamp forests identificationphylogenetic relationshipstree species5.8s rrna nuclear geneBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 4, Pp 1186-1193 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	tripa peat swamp forests identification phylogenetic relationships tree species 5.8s rrna nuclear gene Biology (General) QH301-705.5
spellingShingle	tripa peat swamp forests identification phylogenetic relationships tree species 5.8s rrna nuclear gene Biology (General) QH301-705.5 ZAIRIN THOMY ARDHANA YULISMA ESSY HARNELLY ARIDA SUSILOWATI Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen
description	Authors. 2018. Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen. Biodiversitas 19: 1186-1193. Tripa peat swamp forest is protected areas that have high biodiversity. Nevertheless, in some areas, the damage occurred due to conversions of land function to oil palm plantations. The impact of conversions of peat swamp forest to oil palm plantations has led to biodiversity decreased. Hence, it is important to identify the remain tree species in Tripa peat swamp forest. This study aimed to determinetrees species diversity in Tripa peat swamp forest by using of 5.8S rRNA nuclear gene. Research was conducted at Forest Genetics and Molecular Forestry Laboratory, Faculty of Forestry, IPB from September 2015 to August 2016. Molecular identification consisted of DNA extraction, PCR analysis, and sequencing. The data were analyzed using Bioedit, MEGA 6, BLAST, and ITS2 database. Molecular identification using ITS 1 and ITS 4 primer successfully amplified (the ITS region ITS1-5.8S-ITS2) of 16 trees species from 9 families. BLAST analysis results indicate the presence of 16 species has similar bases sequence with the GeneBank DNA database. The plant species are Branckenridgea palustris (Ochnaceae), Gonystylus sp. (Thymelaeaceae), Tristaniopsis whiteana (Myrtaceae), Syzygium sp.1 (Myrtaceae), Macaranga triloba (Euphorbiaceae), Syzygium garciniifolium (Myrtaceae), Knema intermedia (Myristicaceae), Palaquium ridleyi (Sapotaceae), Palaquium sp. (Sapotaceae), Dyera lowii (Apocynaceae), Elaeocarpus petiolatus (Elaeocarpaceae), Ficus sp. (Moraceae), Syzygium leptostemon (Myrtaceae), Chilocarpus suaveolens (Apocynaceae), Alstonia pneumatophora (Apocynaceae), and Alstonia sp. (Apocynaceae). Phylogeny tree reconstruction using the Neighbor-Joining Method (NJ) showed that 5.8S rRNA nuclear gene was successful as marker for 16 trees species from 9 different families. In addition, the 5.8S also successful for resolving phylogenetic relationships at genus level i.e. Alstonia, Palaquium, Syzygium, Tristaniopsis, Macaranga, Elaeocarpus, and Ficus.
format	article
author	ZAIRIN THOMY ARDHANA YULISMA ESSY HARNELLY ARIDA SUSILOWATI
author_facet	ZAIRIN THOMY ARDHANA YULISMA ESSY HARNELLY ARIDA SUSILOWATI
author_sort	ZAIRIN THOMY
title	Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen
title_short	Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen
title_full	Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen
title_fullStr	Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen
title_full_unstemmed	Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen
title_sort	molecular phylogeny of trees species in tripa peat swamp forest, aceh, indonesia inferred by 5.8s nuclear gen
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/06591ae4cfeb40448401ea62df3ee288
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Molecular phylogeny of trees species in Tripa Peat Swamp Forest, Aceh, Indonesia inferred by 5.8S nuclear gen

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