Quantitative measurements of C-reactive protein using silicon nanowire arrays

Min-Ho Lee, Kuk-Nyung Lee, Suk-Won Jung, Won-Hyo Kim, Kyu-Sik Shin, Woo-Kyeong SeongKorea Electronics Technology Institute, Gyeonggi, KoreaAbstract: A silicon nanowire-based sensor for biological application showed highly desirable electrical responses to either pH changes or receptor-ligand interac...

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Autores principales:	Min-Ho Lee, Kuk-Nyung Lee, Suk-Won Jung, Won-Hyo Kim, Kyu-Sik Shin, Woo-Kyeong Seong
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0660b58968ee444eb926dd5c7f8e9e75
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spelling	oai:doaj.org-article:0660b58968ee444eb926dd5c7f8e9e752021-12-02T01:16:06ZQuantitative measurements of C-reactive protein using silicon nanowire arrays1176-91141178-2013https://doaj.org/article/0660b58968ee444eb926dd5c7f8e9e752008-03-01T00:00:00Zhttp://www.dovepress.com/quantitative-measurements-of-c-reactive-protein-using-silicon-nanowire-a745https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Min-Ho Lee, Kuk-Nyung Lee, Suk-Won Jung, Won-Hyo Kim, Kyu-Sik Shin, Woo-Kyeong SeongKorea Electronics Technology Institute, Gyeonggi, KoreaAbstract: A silicon nanowire-based sensor for biological application showed highly desirable electrical responses to either pH changes or receptor-ligand interactions such as protein disease markers, viruses, and DNA hybridization. Furthermore, because the silicon nanowire can display results in real-time, it may possess superior characteristics for biosensing than those demonstrated in previously studied methods. However, despite its promising potential and advantages, certain process-related limitations of the device, due to its size and material characteristics, need to be addressed. In this article, we suggest possible solutions. We fabricated silicon nanowire using a top-down and low cost micromachining method, and evaluate the sensing of molecules after transfer and surface modifications. Our newly designed method can be used to attach highly ordered nanowires to various substrates, to form a nanowire array device, which needs to follow a series of repetitive steps in conventional fabrication technology based on a vapor-liquid-solid (VLS) method. For evaluation, we demonstrated that our newly fabricated silicon nanowire arrays could detect pH changes as well as streptavidin-biotin binding events. As well as the initial proof-of-principle studies, C-reactive protein binding was measured: electrical signals were changed in a linear fashion with the concentration (1 fM to 1 nM) in PBS containing 1.37 mM of salts. Finally, to address the effects of Debye length, silicon nanowires coupled with antigen proteins underwent electrical signal changes as the salt concentration changed.Keywords: silicon nanowire array, C-reactive protein, vapor-liquid-solid method Min-Ho LeeKuk-Nyung LeeSuk-Won JungWon-Hyo KimKyu-Sik ShinWoo-Kyeong SeongDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2008, Iss Issue 1, Pp 117-124 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Min-Ho Lee Kuk-Nyung Lee Suk-Won Jung Won-Hyo Kim Kyu-Sik Shin Woo-Kyeong Seong Quantitative measurements of C-reactive protein using silicon nanowire arrays
description	Min-Ho Lee, Kuk-Nyung Lee, Suk-Won Jung, Won-Hyo Kim, Kyu-Sik Shin, Woo-Kyeong SeongKorea Electronics Technology Institute, Gyeonggi, KoreaAbstract: A silicon nanowire-based sensor for biological application showed highly desirable electrical responses to either pH changes or receptor-ligand interactions such as protein disease markers, viruses, and DNA hybridization. Furthermore, because the silicon nanowire can display results in real-time, it may possess superior characteristics for biosensing than those demonstrated in previously studied methods. However, despite its promising potential and advantages, certain process-related limitations of the device, due to its size and material characteristics, need to be addressed. In this article, we suggest possible solutions. We fabricated silicon nanowire using a top-down and low cost micromachining method, and evaluate the sensing of molecules after transfer and surface modifications. Our newly designed method can be used to attach highly ordered nanowires to various substrates, to form a nanowire array device, which needs to follow a series of repetitive steps in conventional fabrication technology based on a vapor-liquid-solid (VLS) method. For evaluation, we demonstrated that our newly fabricated silicon nanowire arrays could detect pH changes as well as streptavidin-biotin binding events. As well as the initial proof-of-principle studies, C-reactive protein binding was measured: electrical signals were changed in a linear fashion with the concentration (1 fM to 1 nM) in PBS containing 1.37 mM of salts. Finally, to address the effects of Debye length, silicon nanowires coupled with antigen proteins underwent electrical signal changes as the salt concentration changed.Keywords: silicon nanowire array, C-reactive protein, vapor-liquid-solid method
format	article
author	Min-Ho Lee Kuk-Nyung Lee Suk-Won Jung Won-Hyo Kim Kyu-Sik Shin Woo-Kyeong Seong
author_facet	Min-Ho Lee Kuk-Nyung Lee Suk-Won Jung Won-Hyo Kim Kyu-Sik Shin Woo-Kyeong Seong
author_sort	Min-Ho Lee
title	Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_short	Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_full	Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_fullStr	Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_full_unstemmed	Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_sort	quantitative measurements of c-reactive protein using silicon nanowire arrays
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/0660b58968ee444eb926dd5c7f8e9e75
work_keys_str_mv	AT minholee quantitativemeasurementsofcreactiveproteinusingsiliconnanowirearrays AT kuknyunglee quantitativemeasurementsofcreactiveproteinusingsiliconnanowirearrays AT sukwonjung quantitativemeasurementsofcreactiveproteinusingsiliconnanowirearrays AT wonhyokim quantitativemeasurementsofcreactiveproteinusingsiliconnanowirearrays AT kyusikshin quantitativemeasurementsofcreactiveproteinusingsiliconnanowirearrays AT wookyeongseong quantitativemeasurementsofcreactiveproteinusingsiliconnanowirearrays
_version_	1718403176384692224

Quantitative measurements of C-reactive protein using silicon nanowire arrays

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