Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine

Abstract Bacteria in nature often reside in differentiated communities termed biofilms, which are an active interphase between uni-cellular and multicellular life states for bacteria. Here we demonstrate that the development of B. subtilis biofilms is dependent on the use of glutamine or glutamate a...

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Autores principales:	Tal Hassanov, Iris Karunker, Nitai Steinberg, Ayelet Erez, Ilana Kolodkin-Gal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0661780a71c04fbe9b1b895d6a7474ad
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spelling	oai:doaj.org-article:0661780a71c04fbe9b1b895d6a7474ad2021-12-02T15:08:05ZNovel antibiofilm chemotherapies target nitrogen from glutamate and glutamine10.1038/s41598-018-25401-z2045-2322https://doaj.org/article/0661780a71c04fbe9b1b895d6a7474ad2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25401-zhttps://doaj.org/toc/2045-2322Abstract Bacteria in nature often reside in differentiated communities termed biofilms, which are an active interphase between uni-cellular and multicellular life states for bacteria. Here we demonstrate that the development of B. subtilis biofilms is dependent on the use of glutamine or glutamate as a nitrogen source. We show a differential metabolic requirement within the biofilm; while glutamine is necessary for the dividing cells at the edges, the inner cell mass utilizes lactic acid. Our results indicate that biofilm cells preserve a short-term memory of glutamate metabolism. Finally, we establish that drugs that target glutamine and glutamate utilization restrict biofilm development. Overall, our work reveals a spatial regulation of nitrogen and carbon metabolism within the biofilm, which contributes to the fitness of bacterial complex communities. This acquired metabolic division of labor within biofilm can serve as a target for novel anti-biofilm chemotherapiesTal HassanovIris KarunkerNitai SteinbergAyelet ErezIlana Kolodkin-GalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tal Hassanov Iris Karunker Nitai Steinberg Ayelet Erez Ilana Kolodkin-Gal Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine
description	Abstract Bacteria in nature often reside in differentiated communities termed biofilms, which are an active interphase between uni-cellular and multicellular life states for bacteria. Here we demonstrate that the development of B. subtilis biofilms is dependent on the use of glutamine or glutamate as a nitrogen source. We show a differential metabolic requirement within the biofilm; while glutamine is necessary for the dividing cells at the edges, the inner cell mass utilizes lactic acid. Our results indicate that biofilm cells preserve a short-term memory of glutamate metabolism. Finally, we establish that drugs that target glutamine and glutamate utilization restrict biofilm development. Overall, our work reveals a spatial regulation of nitrogen and carbon metabolism within the biofilm, which contributes to the fitness of bacterial complex communities. This acquired metabolic division of labor within biofilm can serve as a target for novel anti-biofilm chemotherapies
format	article
author	Tal Hassanov Iris Karunker Nitai Steinberg Ayelet Erez Ilana Kolodkin-Gal
author_facet	Tal Hassanov Iris Karunker Nitai Steinberg Ayelet Erez Ilana Kolodkin-Gal
author_sort	Tal Hassanov
title	Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine
title_short	Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine
title_full	Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine
title_fullStr	Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine
title_full_unstemmed	Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine
title_sort	novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0661780a71c04fbe9b1b895d6a7474ad
work_keys_str_mv	AT talhassanov novelantibiofilmchemotherapiestargetnitrogenfromglutamateandglutamine AT iriskarunker novelantibiofilmchemotherapiestargetnitrogenfromglutamateandglutamine AT nitaisteinberg novelantibiofilmchemotherapiestargetnitrogenfromglutamateandglutamine AT ayeleterez novelantibiofilmchemotherapiestargetnitrogenfromglutamateandglutamine AT ilanakolodkingal novelantibiofilmchemotherapiestargetnitrogenfromglutamateandglutamine
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Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine

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