Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion

Our previous studies indicated that ultrasound treatment can increase the anaphylaxis of protein. However, investigation on the anaphylaxis changes of ultrasound-treated α-lactalbumin (ALA) during digestion is lacking. The anaphylaxis of ultrasound-treated ALA and its digesta was investigated. The a...

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Autores principales:	Xumei Wang, Zongcai Tu, Guangxian Liu, Hui Wang, Yueming Hu, Tao Huang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	α-lactalbumin ultrasound in-vitro gastroduodenal digestion anaphylaxis anti-digestive stable peptides high-resolution mass spectrometry Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/06655e2a87da4520935bba656e0edd2d
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spelling	oai:doaj.org-article:06655e2a87da4520935bba656e0edd2d2021-11-25T17:35:18ZInvestigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion10.3390/foods101127602304-8158https://doaj.org/article/06655e2a87da4520935bba656e0edd2d2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2760https://doaj.org/toc/2304-8158Our previous studies indicated that ultrasound treatment can increase the anaphylaxis of protein. However, investigation on the anaphylaxis changes of ultrasound-treated α-lactalbumin (ALA) during digestion is lacking. The anaphylaxis of ultrasound-treated ALA and its digesta was investigated. The anti-digestive stable peptides were identified by high-resolution mass spectrometry. Ultrasound induced the tertiary structure of ALA to unfold and increased its anaphylaxis. During digestion, the anaphylaxis of both gastric and gastroduodenal digesta was further increased. There are two reasons for this phenomenon. On the one hand, linear epitopes played an important role in affecting anaphylaxis compared with the conformational epitope, and some linear epitopes were still retained on the anti-digestive stable peptides produced after gastroduodenal digestion, resulting in increased anaphylaxis after digestion. On the other hand, the presence of intact ALA molecules after digestion still remained strong anaphylaxis. Compared with the digesta of untreated ALA, the digesta of ultrasound-treated ALA possessed higher anaphylaxis. The results indicated that ultrasound increased the anaphylaxis of ALA during digestion.Xumei WangZongcai TuGuangxian LiuHui WangYueming HuTao HuangMDPI AGarticleα-lactalbuminultrasoundin-vitro gastroduodenal digestionanaphylaxisanti-digestive stable peptideshigh-resolution mass spectrometryChemical technologyTP1-1185ENFoods, Vol 10, Iss 2760, p 2760 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	α-lactalbumin ultrasound in-vitro gastroduodenal digestion anaphylaxis anti-digestive stable peptides high-resolution mass spectrometry Chemical technology TP1-1185
spellingShingle	α-lactalbumin ultrasound in-vitro gastroduodenal digestion anaphylaxis anti-digestive stable peptides high-resolution mass spectrometry Chemical technology TP1-1185 Xumei Wang Zongcai Tu Guangxian Liu Hui Wang Yueming Hu Tao Huang Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion
description	Our previous studies indicated that ultrasound treatment can increase the anaphylaxis of protein. However, investigation on the anaphylaxis changes of ultrasound-treated α-lactalbumin (ALA) during digestion is lacking. The anaphylaxis of ultrasound-treated ALA and its digesta was investigated. The anti-digestive stable peptides were identified by high-resolution mass spectrometry. Ultrasound induced the tertiary structure of ALA to unfold and increased its anaphylaxis. During digestion, the anaphylaxis of both gastric and gastroduodenal digesta was further increased. There are two reasons for this phenomenon. On the one hand, linear epitopes played an important role in affecting anaphylaxis compared with the conformational epitope, and some linear epitopes were still retained on the anti-digestive stable peptides produced after gastroduodenal digestion, resulting in increased anaphylaxis after digestion. On the other hand, the presence of intact ALA molecules after digestion still remained strong anaphylaxis. Compared with the digesta of untreated ALA, the digesta of ultrasound-treated ALA possessed higher anaphylaxis. The results indicated that ultrasound increased the anaphylaxis of ALA during digestion.
format	article
author	Xumei Wang Zongcai Tu Guangxian Liu Hui Wang Yueming Hu Tao Huang
author_facet	Xumei Wang Zongcai Tu Guangxian Liu Hui Wang Yueming Hu Tao Huang
author_sort	Xumei Wang
title	Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion
title_short	Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion
title_full	Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion
title_fullStr	Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion
title_full_unstemmed	Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion
title_sort	investigation on the anaphylaxis and anti-digestive stable peptides identification of ultrasound-treated α-lactalbumin during in-vitro gastroduodenal digestion
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/06655e2a87da4520935bba656e0edd2d
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Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion

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