11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography

Identification of primary tumors and metastasis sites is an essential step in cancer diagnostics and the following treatment. Positron emission tomography-computed tomography (PET/CT) is one of the most reliable methods for scanning the whole organism for malignancies. In this work, we synthesized a...

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Autores principales:	Anastasia V. Ozerskaya, Tatiana N. Zamay, Olga S. Kolovskaya, Nikolay A. Tokarev, Kirill V. Belugin, Natalia G. Chanchikova, Oleg N. Badmaev, Galina S. Zamay, Irina A. Shchugoreva, Roman V. Moryachkov, Vladimir N. Zabluda, Vladimir A. Khorzhevskii, Nikolay Shepelevich, Stanislav V. Gappoev, Elena A. Karlova, Anastasia S. Saveleva, Alexander A. Volzhentsev, Anna N. Blagodatova, Kirill A. Lukyanenko, Dmitry V. Veprintsev, Tatyana E. Smolyarova, Felix N. Tomilin, Sergey S. Zamay, Vladimir N. Silnikov, Maxim V. Berezovski, Anna S. Kichkailo
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	11C radiolabeling radiopharmaceuticals PET/CT in vivo imaging DNA aptamers Ehrlich ascites carcinoma Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/066847eacaa5465d896f96a57c20d7dd
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spelling	oai:doaj.org-article:066847eacaa5465d896f96a57c20d7dd2021-11-12T04:31:11Z11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography2162-253110.1016/j.omtn.2021.10.020https://doaj.org/article/066847eacaa5465d896f96a57c20d7dd2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2162253121002638https://doaj.org/toc/2162-2531Identification of primary tumors and metastasis sites is an essential step in cancer diagnostics and the following treatment. Positron emission tomography-computed tomography (PET/CT) is one of the most reliable methods for scanning the whole organism for malignancies. In this work, we synthesized an 11C-labeled oligonucleotide primer and hybridized it to an anti-cancer DNA aptamer. The 11C-aptamer was applied for in vivo imaging of Ehrlich ascites carcinoma and its metastases in mice using PET/CT. The imaging experiments with the 11C-aptamer determined very small primary and secondary tumors of 3 mm2 and less. We also compared 11C imaging with the standard radiotracer, 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG), and found better selectivity of the 11C-aptamer to metastatic lesions in the metabolically active organs than 18F-FDG. 11C radionuclide with an ultra-short (20.38 min) half-life is considered safest for PET/CT imaging and does not cause false-positive results in heart imaging. Its combination with aptamers gives us high-specificity and high-contrast imaging of cancer cells and can be applied for PET/CT-guided drug delivery in cancer therapies.Anastasia V. OzerskayaTatiana N. ZamayOlga S. KolovskayaNikolay A. TokarevKirill V. BeluginNatalia G. ChanchikovaOleg N. BadmaevGalina S. ZamayIrina A. ShchugorevaRoman V. MoryachkovVladimir N. ZabludaVladimir A. KhorzhevskiiNikolay ShepelevichStanislav V. GappoevElena A. KarlovaAnastasia S. SavelevaAlexander A. VolzhentsevAnna N. BlagodatovaKirill A. LukyanenkoDmitry V. VeprintsevTatyana E. SmolyarovaFelix N. TomilinSergey S. ZamayVladimir N. SilnikovMaxim V. BerezovskiAnna S. KichkailoElsevierarticle11C radiolabelingradiopharmaceuticalsPET/CTin vivo imagingDNA aptamersEhrlich ascites carcinomaTherapeutics. PharmacologyRM1-950ENMolecular Therapy: Nucleic Acids, Vol 26, Iss , Pp 1159-1172 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	11C radiolabeling radiopharmaceuticals PET/CT in vivo imaging DNA aptamers Ehrlich ascites carcinoma Therapeutics. Pharmacology RM1-950
spellingShingle	11C radiolabeling radiopharmaceuticals PET/CT in vivo imaging DNA aptamers Ehrlich ascites carcinoma Therapeutics. Pharmacology RM1-950 Anastasia V. Ozerskaya Tatiana N. Zamay Olga S. Kolovskaya Nikolay A. Tokarev Kirill V. Belugin Natalia G. Chanchikova Oleg N. Badmaev Galina S. Zamay Irina A. Shchugoreva Roman V. Moryachkov Vladimir N. Zabluda Vladimir A. Khorzhevskii Nikolay Shepelevich Stanislav V. Gappoev Elena A. Karlova Anastasia S. Saveleva Alexander A. Volzhentsev Anna N. Blagodatova Kirill A. Lukyanenko Dmitry V. Veprintsev Tatyana E. Smolyarova Felix N. Tomilin Sergey S. Zamay Vladimir N. Silnikov Maxim V. Berezovski Anna S. Kichkailo 11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography
description	Identification of primary tumors and metastasis sites is an essential step in cancer diagnostics and the following treatment. Positron emission tomography-computed tomography (PET/CT) is one of the most reliable methods for scanning the whole organism for malignancies. In this work, we synthesized an 11C-labeled oligonucleotide primer and hybridized it to an anti-cancer DNA aptamer. The 11C-aptamer was applied for in vivo imaging of Ehrlich ascites carcinoma and its metastases in mice using PET/CT. The imaging experiments with the 11C-aptamer determined very small primary and secondary tumors of 3 mm2 and less. We also compared 11C imaging with the standard radiotracer, 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG), and found better selectivity of the 11C-aptamer to metastatic lesions in the metabolically active organs than 18F-FDG. 11C radionuclide with an ultra-short (20.38 min) half-life is considered safest for PET/CT imaging and does not cause false-positive results in heart imaging. Its combination with aptamers gives us high-specificity and high-contrast imaging of cancer cells and can be applied for PET/CT-guided drug delivery in cancer therapies.
format	article
author	Anastasia V. Ozerskaya Tatiana N. Zamay Olga S. Kolovskaya Nikolay A. Tokarev Kirill V. Belugin Natalia G. Chanchikova Oleg N. Badmaev Galina S. Zamay Irina A. Shchugoreva Roman V. Moryachkov Vladimir N. Zabluda Vladimir A. Khorzhevskii Nikolay Shepelevich Stanislav V. Gappoev Elena A. Karlova Anastasia S. Saveleva Alexander A. Volzhentsev Anna N. Blagodatova Kirill A. Lukyanenko Dmitry V. Veprintsev Tatyana E. Smolyarova Felix N. Tomilin Sergey S. Zamay Vladimir N. Silnikov Maxim V. Berezovski Anna S. Kichkailo
author_facet	Anastasia V. Ozerskaya Tatiana N. Zamay Olga S. Kolovskaya Nikolay A. Tokarev Kirill V. Belugin Natalia G. Chanchikova Oleg N. Badmaev Galina S. Zamay Irina A. Shchugoreva Roman V. Moryachkov Vladimir N. Zabluda Vladimir A. Khorzhevskii Nikolay Shepelevich Stanislav V. Gappoev Elena A. Karlova Anastasia S. Saveleva Alexander A. Volzhentsev Anna N. Blagodatova Kirill A. Lukyanenko Dmitry V. Veprintsev Tatyana E. Smolyarova Felix N. Tomilin Sergey S. Zamay Vladimir N. Silnikov Maxim V. Berezovski Anna S. Kichkailo
author_sort	Anastasia V. Ozerskaya
title	11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography
title_short	11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography
title_full	11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography
title_fullStr	11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography
title_full_unstemmed	11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography
title_sort	11c-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/066847eacaa5465d896f96a57c20d7dd
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11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography

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