Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera

ABSTRACT Opsonophagocytic assays are used to measure functional antibodies important in protection against pneumococcal capsular antigens. There have been efforts to standardize these methods, as the assays are commonly used to measure vaccine immunogenicity. We report here the results from three in...

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Autores principales:	A. Balloch, L. Roalfe, N. Ekstrom, C. D. Nguyen, L. Spry, R. A. Marimla, P. V. Licciardi, D. Goldblatt, E. K. Mulholland
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	immunization MOPA pneumococcal IgG Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/066e8d3bb36c49318857a1a4397b162f
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spelling	oai:doaj.org-article:066e8d3bb36c49318857a1a4397b162f2021-11-15T15:22:14ZInterlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera10.1128/mSphere.00070-182379-5042https://doaj.org/article/066e8d3bb36c49318857a1a4397b162f2018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00070-18https://doaj.org/toc/2379-5042ABSTRACT Opsonophagocytic assays are used to measure functional antibodies important in protection against pneumococcal capsular antigens. There have been efforts to standardize these methods, as the assays are commonly used to measure vaccine immunogenicity. We report here the results from three international laboratories using their own methods, based on the recommended WHO standard method. We tested 30 pediatric sera, before and after administration of a 13-valent conjugate pneumococcal vaccine, against all 13 serotypes. The three laboratories demonstrated good agreement using their own standardized multiplex opsonophagocytosis assay protocols, particularly postimmunization for those serotypes in the vaccine. While serotype-specific IgG methods have already been internationally standardized and are currently used as a measure of vaccine immunogenicity, this report demonstrates that despite minor differences in methods and a minor variation in response to nonvaccine serotypes, the results from opsonophagocytic assays across the three laboratories may be compared with confidence. IMPORTANCE When measuring a functional antibody response to pneumococcal immunization, it is imperative that a specific, reproducible, accurate, and standardized assay with acceptable inter- and intra-assay variation be advocated internationally to allow for meaningful comparison of results between laboratories. We report here the results of a collaboration between 3 international laboratories testing 30 pediatric samples against the 13 serotypes in Prevenar13.A. BallochL. RoalfeN. EkstromC. D. NguyenL. SpryR. A. MarimlaP. V. LicciardiD. GoldblattE. K. MulhollandAmerican Society for MicrobiologyarticleimmunizationMOPApneumococcal IgGMicrobiologyQR1-502ENmSphere, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunization MOPA pneumococcal IgG Microbiology QR1-502
spellingShingle	immunization MOPA pneumococcal IgG Microbiology QR1-502 A. Balloch L. Roalfe N. Ekstrom C. D. Nguyen L. Spry R. A. Marimla P. V. Licciardi D. Goldblatt E. K. Mulholland Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera
description	ABSTRACT Opsonophagocytic assays are used to measure functional antibodies important in protection against pneumococcal capsular antigens. There have been efforts to standardize these methods, as the assays are commonly used to measure vaccine immunogenicity. We report here the results from three international laboratories using their own methods, based on the recommended WHO standard method. We tested 30 pediatric sera, before and after administration of a 13-valent conjugate pneumococcal vaccine, against all 13 serotypes. The three laboratories demonstrated good agreement using their own standardized multiplex opsonophagocytosis assay protocols, particularly postimmunization for those serotypes in the vaccine. While serotype-specific IgG methods have already been internationally standardized and are currently used as a measure of vaccine immunogenicity, this report demonstrates that despite minor differences in methods and a minor variation in response to nonvaccine serotypes, the results from opsonophagocytic assays across the three laboratories may be compared with confidence. IMPORTANCE When measuring a functional antibody response to pneumococcal immunization, it is imperative that a specific, reproducible, accurate, and standardized assay with acceptable inter- and intra-assay variation be advocated internationally to allow for meaningful comparison of results between laboratories. We report here the results of a collaboration between 3 international laboratories testing 30 pediatric samples against the 13 serotypes in Prevenar13.
format	article
author	A. Balloch L. Roalfe N. Ekstrom C. D. Nguyen L. Spry R. A. Marimla P. V. Licciardi D. Goldblatt E. K. Mulholland
author_facet	A. Balloch L. Roalfe N. Ekstrom C. D. Nguyen L. Spry R. A. Marimla P. V. Licciardi D. Goldblatt E. K. Mulholland
author_sort	A. Balloch
title	Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera
title_short	Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera
title_full	Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera
title_fullStr	Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera
title_full_unstemmed	Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera
title_sort	interlaboratory comparison of the pneumococcal multiplex opsonophagocytic assays and their level of agreement for determination of antibody function in pediatric sera
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/066e8d3bb36c49318857a1a4397b162f
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Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera

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