Genetic Diversity of Staphylococcus aureus Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan

Staphylococcus aureus is an important healthcare-associated bacterium that causes a multitude of infections in humans such as superficial skin and soft tissue infections, necrotizing pneumonia, foodborne illnesses and postsurgical infections. Treatment of S. aureus</i&...

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Autores principales:	Muhammad Ali Syed, Bushra Jamil, Hazem Ramadan, Maria Rukan, Shahzad Ali, Shahid Ahmad Abbasi, Tiffanie A. Woodley, Charlene R. Jackson
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Staphylococcus aureus</i> antibiotic resistance multilocus sequence typing pulsed-field gel electrophoresis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/067a9c63777e477abd4653a60326b46d
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spelling	oai:doaj.org-article:067a9c63777e477abd4653a60326b46d2021-11-25T18:24:57ZGenetic Diversity of <i>Staphylococcus aureus</i> Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan10.3390/microorganisms91123012076-2607https://doaj.org/article/067a9c63777e477abd4653a60326b46d2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2301https://doaj.org/toc/2076-2607<i>Staphylococcus aureus</i> is an important healthcare-associated bacterium that causes a multitude of infections in humans such as superficial skin and soft tissue infections, necrotizing pneumonia, foodborne illnesses and postsurgical infections. Treatment of <i>S. aureus</i> infections has become more complicated due to the emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA), some of which are multidrug resistant. The present study aimed to characterize <i>S. aureus</i> isolates from a tertiary care hospital in the Rawalpindi district of Pakistan. Staphylococci were isolated from 300 clinical samples collected from January 2018 to January 2019 and <i>S. aureus</i> isolates were tested for antimicrobial susceptibility and analyzed using Pulsed-Field Gel Electrophoresis (PFGE), Multi-Locus Sequence Typing (MLST), staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>) and <i>spa</i> typing. Approximately 25.3% (76/300) of the clinical samples were positive for <i>S. aureus</i>; of those, 88.2% (67/76) were <i>mec</i>A+ (MRSA). In addition to the β-lactam antibiotics, high levels of resistance were also found to the fluoroquinolones (ciprofloxacin, gatifloxacin and levofloxacin (73.7% each)). Of the 23 different <i>spa</i> types identified, the majority of isolates belonged to <i>spa</i> type t632 and t657 (9/66; 13.6% each <i>spa</i> type). ST772-t657 (Bengal Bay clone) was the most commonly identified clone in this study although other clones circulating around different regions of the world were also found indicating the diversity in MRSA isolates from this area of Pakistan. This study emphasizes the need to monitor MRSA in the clinical setting for improved infection control and treatment options.Muhammad Ali SyedBushra JamilHazem RamadanMaria RukanShahzad AliShahid Ahmad AbbasiTiffanie A. WoodleyCharlene R. JacksonMDPI AGarticle<i>Staphylococcus aureus</i>antibiotic resistancemultilocus sequence typingpulsed-field gel electrophoresisBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2301, p 2301 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Staphylococcus aureus</i> antibiotic resistance multilocus sequence typing pulsed-field gel electrophoresis Biology (General) QH301-705.5
spellingShingle	<i>Staphylococcus aureus</i> antibiotic resistance multilocus sequence typing pulsed-field gel electrophoresis Biology (General) QH301-705.5 Muhammad Ali Syed Bushra Jamil Hazem Ramadan Maria Rukan Shahzad Ali Shahid Ahmad Abbasi Tiffanie A. Woodley Charlene R. Jackson Genetic Diversity of <i>Staphylococcus aureus</i> Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan
description	<i>Staphylococcus aureus</i> is an important healthcare-associated bacterium that causes a multitude of infections in humans such as superficial skin and soft tissue infections, necrotizing pneumonia, foodborne illnesses and postsurgical infections. Treatment of <i>S. aureus</i> infections has become more complicated due to the emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA), some of which are multidrug resistant. The present study aimed to characterize <i>S. aureus</i> isolates from a tertiary care hospital in the Rawalpindi district of Pakistan. Staphylococci were isolated from 300 clinical samples collected from January 2018 to January 2019 and <i>S. aureus</i> isolates were tested for antimicrobial susceptibility and analyzed using Pulsed-Field Gel Electrophoresis (PFGE), Multi-Locus Sequence Typing (MLST), staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>) and <i>spa</i> typing. Approximately 25.3% (76/300) of the clinical samples were positive for <i>S. aureus</i>; of those, 88.2% (67/76) were <i>mec</i>A+ (MRSA). In addition to the β-lactam antibiotics, high levels of resistance were also found to the fluoroquinolones (ciprofloxacin, gatifloxacin and levofloxacin (73.7% each)). Of the 23 different <i>spa</i> types identified, the majority of isolates belonged to <i>spa</i> type t632 and t657 (9/66; 13.6% each <i>spa</i> type). ST772-t657 (Bengal Bay clone) was the most commonly identified clone in this study although other clones circulating around different regions of the world were also found indicating the diversity in MRSA isolates from this area of Pakistan. This study emphasizes the need to monitor MRSA in the clinical setting for improved infection control and treatment options.
format	article
author	Muhammad Ali Syed Bushra Jamil Hazem Ramadan Maria Rukan Shahzad Ali Shahid Ahmad Abbasi Tiffanie A. Woodley Charlene R. Jackson
author_facet	Muhammad Ali Syed Bushra Jamil Hazem Ramadan Maria Rukan Shahzad Ali Shahid Ahmad Abbasi Tiffanie A. Woodley Charlene R. Jackson
author_sort	Muhammad Ali Syed
title	Genetic Diversity of <i>Staphylococcus aureus</i> Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan
title_short	Genetic Diversity of <i>Staphylococcus aureus</i> Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan
title_full	Genetic Diversity of <i>Staphylococcus aureus</i> Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan
title_fullStr	Genetic Diversity of <i>Staphylococcus aureus</i> Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan
title_full_unstemmed	Genetic Diversity of <i>Staphylococcus aureus</i> Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan
title_sort	genetic diversity of <i>staphylococcus aureus</i> strains from a tertiary care hospital in rawalpindi, pakistan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/067a9c63777e477abd4653a60326b46d
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Genetic Diversity of <i>Staphylococcus aureus</i> Strains from a Tertiary Care Hospital in Rawalpindi, Pakistan

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