Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis

ABSTRACT It is generally believed that the microbiome plays a role in the pathophysiology of chronic rhinosinusitis (CRS), though its exact contribution to disease development and severity remains unclear. Here, samples were collected from the anterior nares, nasopharynx, and maxillary and ethmoid s...

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Autores principales:	Ilke De Boeck, Stijn Wittouck, Katleen Martens, Jos Claes, Mark Jorissen, Brecht Steelant, Marianne F. L. van den Broek, Sven F. Seys, Peter W. Hellings, Olivier M. Vanderveken, Sarah Lebeer
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	microbiome upper respiratory tract chronic rhinosinusitis sinus pathobionts Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/067c722317fe4cf9b907f12cc6f19cca
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spelling	oai:doaj.org-article:067c722317fe4cf9b907f12cc6f19cca2021-11-15T15:22:24ZAnterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis10.1128/mSphere.00532-192379-5042https://doaj.org/article/067c722317fe4cf9b907f12cc6f19cca2019-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00532-19https://doaj.org/toc/2379-5042ABSTRACT It is generally believed that the microbiome plays a role in the pathophysiology of chronic rhinosinusitis (CRS), though its exact contribution to disease development and severity remains unclear. Here, samples were collected from the anterior nares, nasopharynx, and maxillary and ethmoid sinuses of 190 CRS patients and from the anterior nares and nasopharynx of 100 controls. Microbial communities were analyzed by Illumina sequencing of the V4 region of 16S rRNA. The phenotype and patient characteristics were documented, and several serum inflammatory markers were measured. Our data indicate a rather strong continuity for the microbiome in the different upper respiratory tract (URT) niches in CRS patients, with the microbiome in the anterior nares being most similar to the sinus microbiome. Bacterial diversity was reduced in CRS patients without nasal polyps compared to that in the controls but not in CRS patients with nasal polyps. Statistically significant differences in the presence/absence or relative abundance of several taxa were found between the CRS patients and the healthy controls. Of these, Dolosigranulum pigrum was clearly more associated with URT samples from healthy subjects, while the Corynebacterium tuberculostearicum, Haemophilus influenzae/H. aegyptius, and Staphylococcus taxa were found to be potential pathobionts in CRS patients. However, CRS versus health as a predictor explained only 1 to 2% of the variance in the microbiome profiles in an adonis model. A history of functional endoscopic sinus surgery, age, and sex also showed a minor association. This study thus indicates that functional studies on the potential beneficial versus pathogenic activity of the different indicator taxa found here are needed to further understand the pathology of CRS and its different phenotypes. (This study has been registered at ClinicalTrials.gov under identifier NCT02933983.) IMPORTANCE There is a clear need to better understand the pathology and specific microbiome features in chronic rhinosinusitis patients, but little is known about the bacterial topography and continuity between the different niches of the upper respiratory tract. Our work showed that the anterior nares could be an important reservoir for potential sinus pathobionts. This has implications for the diagnosis, prevention, and treatment of CRS. In addition, we found a potential pathogenic role for the Corynebacterium tuberculostearicum, Haemophilus influenzae/H. aegyptius, and Staphylococcus taxa and a potential beneficial role for Dolosigranulum. Finally, a decreased microbiome diversity was observed in patients with chronic rhinosinusitis without nasal polyps compared to that in healthy controls but not in chronic rhinosinusitis patients with nasal polyps. This suggests a potential role for the microbiome in disease development or progression of mainly this phenotype.Ilke De BoeckStijn WittouckKatleen MartensJos ClaesMark JorissenBrecht SteelantMarianne F. L. van den BroekSven F. SeysPeter W. HellingsOlivier M. VandervekenSarah LebeerAmerican Society for Microbiologyarticlemicrobiomeupper respiratory tractchronic rhinosinusitissinus pathobiontsMicrobiologyQR1-502ENmSphere, Vol 4, Iss 6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbiome upper respiratory tract chronic rhinosinusitis sinus pathobionts Microbiology QR1-502
spellingShingle	microbiome upper respiratory tract chronic rhinosinusitis sinus pathobionts Microbiology QR1-502 Ilke De Boeck Stijn Wittouck Katleen Martens Jos Claes Mark Jorissen Brecht Steelant Marianne F. L. van den Broek Sven F. Seys Peter W. Hellings Olivier M. Vanderveken Sarah Lebeer Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis
description	ABSTRACT It is generally believed that the microbiome plays a role in the pathophysiology of chronic rhinosinusitis (CRS), though its exact contribution to disease development and severity remains unclear. Here, samples were collected from the anterior nares, nasopharynx, and maxillary and ethmoid sinuses of 190 CRS patients and from the anterior nares and nasopharynx of 100 controls. Microbial communities were analyzed by Illumina sequencing of the V4 region of 16S rRNA. The phenotype and patient characteristics were documented, and several serum inflammatory markers were measured. Our data indicate a rather strong continuity for the microbiome in the different upper respiratory tract (URT) niches in CRS patients, with the microbiome in the anterior nares being most similar to the sinus microbiome. Bacterial diversity was reduced in CRS patients without nasal polyps compared to that in the controls but not in CRS patients with nasal polyps. Statistically significant differences in the presence/absence or relative abundance of several taxa were found between the CRS patients and the healthy controls. Of these, Dolosigranulum pigrum was clearly more associated with URT samples from healthy subjects, while the Corynebacterium tuberculostearicum, Haemophilus influenzae/H. aegyptius, and Staphylococcus taxa were found to be potential pathobionts in CRS patients. However, CRS versus health as a predictor explained only 1 to 2% of the variance in the microbiome profiles in an adonis model. A history of functional endoscopic sinus surgery, age, and sex also showed a minor association. This study thus indicates that functional studies on the potential beneficial versus pathogenic activity of the different indicator taxa found here are needed to further understand the pathology of CRS and its different phenotypes. (This study has been registered at ClinicalTrials.gov under identifier NCT02933983.) IMPORTANCE There is a clear need to better understand the pathology and specific microbiome features in chronic rhinosinusitis patients, but little is known about the bacterial topography and continuity between the different niches of the upper respiratory tract. Our work showed that the anterior nares could be an important reservoir for potential sinus pathobionts. This has implications for the diagnosis, prevention, and treatment of CRS. In addition, we found a potential pathogenic role for the Corynebacterium tuberculostearicum, Haemophilus influenzae/H. aegyptius, and Staphylococcus taxa and a potential beneficial role for Dolosigranulum. Finally, a decreased microbiome diversity was observed in patients with chronic rhinosinusitis without nasal polyps compared to that in healthy controls but not in chronic rhinosinusitis patients with nasal polyps. This suggests a potential role for the microbiome in disease development or progression of mainly this phenotype.
format	article
author	Ilke De Boeck Stijn Wittouck Katleen Martens Jos Claes Mark Jorissen Brecht Steelant Marianne F. L. van den Broek Sven F. Seys Peter W. Hellings Olivier M. Vanderveken Sarah Lebeer
author_facet	Ilke De Boeck Stijn Wittouck Katleen Martens Jos Claes Mark Jorissen Brecht Steelant Marianne F. L. van den Broek Sven F. Seys Peter W. Hellings Olivier M. Vanderveken Sarah Lebeer
author_sort	Ilke De Boeck
title	Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis
title_short	Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis
title_full	Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis
title_fullStr	Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis
title_full_unstemmed	Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis
title_sort	anterior nares diversity and pathobionts represent sinus microbiome in chronic rhinosinusitis
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/067c722317fe4cf9b907f12cc6f19cca
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Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis

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