Polyunsaturated fatty acid status of leukocyte membranes in COPD patients

The aim of the study was to analyze n-3 and n-6 polyunsaturated fatty acid (PUFA) profile of blood leukocyte cytomembranes in mild and moderate COPD, and to establish possible role of these fatty acids in COPD progression. The study involved 110 patients with mild disease (n = 60) and moderate COPD...

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Autores principales:	Yu. K. Denisenko, T. P. Novgorodtseva, V. V. Knyshova, M. V. Antonyuk
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	fatty acids polyunsaturated fatty acids leukocytes cell membrane chronic inflammation copd Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/0681190137f642a68f21be8eeeaed60a
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spelling	oai:doaj.org-article:0681190137f642a68f21be8eeeaed60a2021-11-18T08:03:50ZPolyunsaturated fatty acid status of leukocyte membranes in COPD patients1563-06252313-741X10.15789/1563-0625-PFA-1980https://doaj.org/article/0681190137f642a68f21be8eeeaed60a2021-03-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1980https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of the study was to analyze n-3 and n-6 polyunsaturated fatty acid (PUFA) profile of blood leukocyte cytomembranes in mild and moderate COPD, and to establish possible role of these fatty acids in COPD progression. The study involved 110 patients with mild disease (n = 60) and moderate COPD (50 patients), at average age of 57.5±4.8 years old. The control group consisted of 32 practically healthy non-smoking people with normal pulmonary function (average age 42.0±3.4 years). The immunological study included flow cytometric determination of blood immune cell subpopulations, i.e., T lymphocytes (CD3+), T helper cells (CD4+), cytotoxic T lymphocytes (CD8+), and B cells (CD19+) using Becton Dickinson machine (USA). Fatty acid methyl esters redissolved in hexane were analyzed using “Shimadzu GC-2010” gas-liquid chromato-graphic system (Japan). Analysis of the polyunsaturated fatty acid profile of leukocyte membranes in COPD patients revealed a reduced concentration of essential linoleic acid (18:2n-6) regardless of the disease severity. The leukocyte membrane levels of the long-chain n-6 PUFAs, such as dihomo-γ-linolenic acid (20:3n-6), arachidonic acid (20:4n-6), and docosatetraenoic acid (22:4n-6), were elevated in patients with COPD compared with the control group. However, the concentration of the described above n-6 PUFAs in leukocyte membranes was increased in patients with moderate COPD compared to the patients with mild COPD. The significant deficiency of a physiologically important n-3 PUFA, eicosapentaenoic acid (20:5n- 3), in leukocyte membranes in the COPD patients was revealed. In turn, the low level of 20:5n-3 could result from the deficiency of its precursor, docosahexaenoic acid (22:6n-3). The results of the study indicate the modification in the PUFA composition of blood leukocyte membranes in the patients with COPD. It was shown that altered composition of long-chain fatty acid of leukocyte membranes emerges already at the early stage of the disease. Therefore, the imbalance in fatty acids composition of leukocytes makes a significant contribution to the development and the progression of COPD.Yu. K. DenisenkoT. P. NovgorodtsevaV. V. KnyshovaM. V. AntonyukSPb RAACIarticlefatty acidspolyunsaturated fatty acidsleukocytescell membranechronic inflammationcopdImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 1, Pp 157-162 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	fatty acids polyunsaturated fatty acids leukocytes cell membrane chronic inflammation copd Immunologic diseases. Allergy RC581-607
spellingShingle	fatty acids polyunsaturated fatty acids leukocytes cell membrane chronic inflammation copd Immunologic diseases. Allergy RC581-607 Yu. K. Denisenko T. P. Novgorodtseva V. V. Knyshova M. V. Antonyuk Polyunsaturated fatty acid status of leukocyte membranes in COPD patients
description	The aim of the study was to analyze n-3 and n-6 polyunsaturated fatty acid (PUFA) profile of blood leukocyte cytomembranes in mild and moderate COPD, and to establish possible role of these fatty acids in COPD progression. The study involved 110 patients with mild disease (n = 60) and moderate COPD (50 patients), at average age of 57.5±4.8 years old. The control group consisted of 32 practically healthy non-smoking people with normal pulmonary function (average age 42.0±3.4 years). The immunological study included flow cytometric determination of blood immune cell subpopulations, i.e., T lymphocytes (CD3+), T helper cells (CD4+), cytotoxic T lymphocytes (CD8+), and B cells (CD19+) using Becton Dickinson machine (USA). Fatty acid methyl esters redissolved in hexane were analyzed using “Shimadzu GC-2010” gas-liquid chromato-graphic system (Japan). Analysis of the polyunsaturated fatty acid profile of leukocyte membranes in COPD patients revealed a reduced concentration of essential linoleic acid (18:2n-6) regardless of the disease severity. The leukocyte membrane levels of the long-chain n-6 PUFAs, such as dihomo-γ-linolenic acid (20:3n-6), arachidonic acid (20:4n-6), and docosatetraenoic acid (22:4n-6), were elevated in patients with COPD compared with the control group. However, the concentration of the described above n-6 PUFAs in leukocyte membranes was increased in patients with moderate COPD compared to the patients with mild COPD. The significant deficiency of a physiologically important n-3 PUFA, eicosapentaenoic acid (20:5n- 3), in leukocyte membranes in the COPD patients was revealed. In turn, the low level of 20:5n-3 could result from the deficiency of its precursor, docosahexaenoic acid (22:6n-3). The results of the study indicate the modification in the PUFA composition of blood leukocyte membranes in the patients with COPD. It was shown that altered composition of long-chain fatty acid of leukocyte membranes emerges already at the early stage of the disease. Therefore, the imbalance in fatty acids composition of leukocytes makes a significant contribution to the development and the progression of COPD.
format	article
author	Yu. K. Denisenko T. P. Novgorodtseva V. V. Knyshova M. V. Antonyuk
author_facet	Yu. K. Denisenko T. P. Novgorodtseva V. V. Knyshova M. V. Antonyuk
author_sort	Yu. K. Denisenko
title	Polyunsaturated fatty acid status of leukocyte membranes in COPD patients
title_short	Polyunsaturated fatty acid status of leukocyte membranes in COPD patients
title_full	Polyunsaturated fatty acid status of leukocyte membranes in COPD patients
title_fullStr	Polyunsaturated fatty acid status of leukocyte membranes in COPD patients
title_full_unstemmed	Polyunsaturated fatty acid status of leukocyte membranes in COPD patients
title_sort	polyunsaturated fatty acid status of leukocyte membranes in copd patients
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/0681190137f642a68f21be8eeeaed60a
work_keys_str_mv	AT yukdenisenko polyunsaturatedfattyacidstatusofleukocytemembranesincopdpatients AT tpnovgorodtseva polyunsaturatedfattyacidstatusofleukocytemembranesincopdpatients AT vvknyshova polyunsaturatedfattyacidstatusofleukocytemembranesincopdpatients AT mvantonyuk polyunsaturatedfattyacidstatusofleukocytemembranesincopdpatients
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Polyunsaturated fatty acid status of leukocyte membranes in COPD patients

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