Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine

This work was designed to provide high protective, long-lasting immunity against FMD by enhancing the immunogenicity of the trivalent FMD vaccine using carbomer as adjuvant using G. pigs as an alternative cheapest animal model for quality control testing of the prepared FMD vaccines formulae. Guinea...

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Autores principales:	Walaa S. Shabana, Abeer Abd EL-Sadek
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	adjuvants carbomer fmd montanide isa206 Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.154578 https://doaj.org/article/06816c5d517b49dba6b6e22fcb1a8e82
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spelling	oai:doaj.org-article:06816c5d517b49dba6b6e22fcb1a8e822021-12-02T18:03:08ZEffect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccinehttps://dx.doi.org/10.21608/javs.2021.1545781687-40722090-3308https://doaj.org/article/06816c5d517b49dba6b6e22fcb1a8e822021-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_154578.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308This work was designed to provide high protective, long-lasting immunity against FMD by enhancing the immunogenicity of the trivalent FMD vaccine using carbomer as adjuvant using G. pigs as an alternative cheapest animal model for quality control testing of the prepared FMD vaccines formulae. Guinea pigs were chosen as experimental models to develop concepts and techniques to study the PD50 of FMD vaccines because of the similarities of clinical symptoms in these animals to those of swine and cattle to saving cost, three different formulae of inactivated trivalent FMD vaccine including serotypes O Pan Asia2, A Iran O5 and SAT2/EGY/2012 were prepared as formula 1- (50% carbomer to 50% antigen); formula 2 (50% Montanide ISA 206 to 50%antigen) and formula 3- (25% Montanide ISA 206 and 25% carbomer with 50 % antigen). All of such formulae were found to be free from foreign contaminants, safe and potent, showing no postvaccinal reactions and high protective levels of specific FMD antibodies in Guinea pigs. Each vaccine formula's immunogenicity was determined by estimation of 50% Guinea pig protective dose (GPPD50) and monitoring of the humeral antibody response of vaccinated G. Pig groups. It was found that Montanide oils 206 with carbomer is the best vaccine formula, followed by Montanide oils 206 and finally carbomer which give early short-lasting immunity. Walaa S. ShabanaAbeer Abd EL-SadekEgyptian Society for Animal Managementarticleadjuvantscarbomerfmdmontanide isa206ZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 2, Pp 27-36 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adjuvants carbomer fmd montanide isa206 Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	adjuvants carbomer fmd montanide isa206 Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Walaa S. Shabana Abeer Abd EL-Sadek Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine
description	This work was designed to provide high protective, long-lasting immunity against FMD by enhancing the immunogenicity of the trivalent FMD vaccine using carbomer as adjuvant using G. pigs as an alternative cheapest animal model for quality control testing of the prepared FMD vaccines formulae. Guinea pigs were chosen as experimental models to develop concepts and techniques to study the PD50 of FMD vaccines because of the similarities of clinical symptoms in these animals to those of swine and cattle to saving cost, three different formulae of inactivated trivalent FMD vaccine including serotypes O Pan Asia2, A Iran O5 and SAT2/EGY/2012 were prepared as formula 1- (50% carbomer to 50% antigen); formula 2 (50% Montanide ISA 206 to 50%antigen) and formula 3- (25% Montanide ISA 206 and 25% carbomer with 50 % antigen). All of such formulae were found to be free from foreign contaminants, safe and potent, showing no postvaccinal reactions and high protective levels of specific FMD antibodies in Guinea pigs. Each vaccine formula's immunogenicity was determined by estimation of 50% Guinea pig protective dose (GPPD50) and monitoring of the humeral antibody response of vaccinated G. Pig groups. It was found that Montanide oils 206 with carbomer is the best vaccine formula, followed by Montanide oils 206 and finally carbomer which give early short-lasting immunity.
format	article
author	Walaa S. Shabana Abeer Abd EL-Sadek
author_facet	Walaa S. Shabana Abeer Abd EL-Sadek
author_sort	Walaa S. Shabana
title	Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine
title_short	Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine
title_full	Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine
title_fullStr	Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine
title_full_unstemmed	Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine
title_sort	effect of carbomer as an adjuvant for enhancement of immune-response against fmd vaccine
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.154578 https://doaj.org/article/06816c5d517b49dba6b6e22fcb1a8e82
work_keys_str_mv	AT walaasshabana effectofcarbomerasanadjuvantforenhancementofimmuneresponseagainstfmdvaccine AT abeerabdelsadek effectofcarbomerasanadjuvantforenhancementofimmuneresponseagainstfmdvaccine
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Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine

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