Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution. Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequenc...

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Autores principales:	Rajendra Kumar Gurumurthy, Naveen Kumar, Cindrilla Chumduri
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell isolation Single Cell RNAseq Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/0685779c3d9e4bed9cc21a1fe1ed29f1
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spelling	oai:doaj.org-article:0685779c3d9e4bed9cc21a1fe1ed29f12021-11-20T05:13:57ZOptimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing2666-166710.1016/j.xpro.2021.100970https://doaj.org/article/0685779c3d9e4bed9cc21a1fe1ed29f12021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006766https://doaj.org/toc/2666-1667Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution. Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequencing results. Here, we describe an optimized protocol for isolating the female reproductive tract (FRT), dissecting different FRT regions, and preparing high-viability single cells from the uterine endocervix and ectocervix to generate a complete molecular cell atlas by scRNA-seq for studying normal physiology and disease.For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).Rajendra Kumar GurumurthyNaveen KumarCindrilla ChumduriElsevierarticleCell isolationSingle CellRNAseqScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100970- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell isolation Single Cell RNAseq Science (General) Q1-390
spellingShingle	Cell isolation Single Cell RNAseq Science (General) Q1-390 Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing
description	Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution. Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequencing results. Here, we describe an optimized protocol for isolating the female reproductive tract (FRT), dissecting different FRT regions, and preparing high-viability single cells from the uterine endocervix and ectocervix to generate a complete molecular cell atlas by scRNA-seq for studying normal physiology and disease.For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).
format	article
author	Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri
author_facet	Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri
author_sort	Rajendra Kumar Gurumurthy
title	Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing
title_short	Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing
title_full	Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing
title_fullStr	Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing
title_full_unstemmed	Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing
title_sort	optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell rna sequencing
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0685779c3d9e4bed9cc21a1fe1ed29f1
work_keys_str_mv	AT rajendrakumargurumurthy optimizedprotocolforisolationofhighqualitysinglecellsfromthefemalemousereproductivetracttissuesforsinglecellrnasequencing AT naveenkumar optimizedprotocolforisolationofhighqualitysinglecellsfromthefemalemousereproductivetracttissuesforsinglecellrnasequencing AT cindrillachumduri optimizedprotocolforisolationofhighqualitysinglecellsfromthefemalemousereproductivetracttissuesforsinglecellrnasequencing
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Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

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