Exploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)

Finding the right enzyme experiment can be problematic, depending what one is trying to show, what supplies and equipment are available, and the time one can devote to the topic. I’ve developed simple and inexpensive labs for looking at catalase and invertase activity using yeast encapsulated in sod...

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Autor principal:	Pamela J. Bryer
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/06883c16b9eb40588109f217d1b72bb1
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spelling	oai:doaj.org-article:06883c16b9eb40588109f217d1b72bb12021-11-15T15:13:58ZExploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)10.1128/jmbe.v17i3.11801935-78851935-7877https://doaj.org/article/06883c16b9eb40588109f217d1b72bb12016-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v17i3.1180https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Finding the right enzyme experiment can be problematic, depending what one is trying to show, what supplies and equipment are available, and the time one can devote to the topic. I’ve developed simple and inexpensive labs for looking at catalase and invertase activity using yeast encapsulated in sodium alginate. Single-celled yeast, Saccharomyces cerevisiae, are encapsulated in sodium alginate, a readily available extract from brown algae that, when it comes in contact with calcium chloride (CaCl2), forms a sphere or “bead.” These spheres may then be put into a solution containing substrate to test for enzyme activity. The spheres are easy to manipulate, one doesn’t have the variability and mess of a yeast solution, and since there are no cells in solution, there is nothing to interfere with the various assay methods one might want to use to test for product. The graduated cylinder method for testing catalase activity introduced here is especially good for collecting large amounts of data that enable students to use statistics and, unlike similar yeast catalase experiments using paper disks and a yeast solution, the yeast spheres are easy to manipulate and there is very little variability. I have used this procedure with students in class and with teachers in workshops with positive results and comments.Pamela J. BryerAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 17, Iss 3, Pp 490-491 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Pamela J. Bryer Exploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)
description	Finding the right enzyme experiment can be problematic, depending what one is trying to show, what supplies and equipment are available, and the time one can devote to the topic. I’ve developed simple and inexpensive labs for looking at catalase and invertase activity using yeast encapsulated in sodium alginate. Single-celled yeast, Saccharomyces cerevisiae, are encapsulated in sodium alginate, a readily available extract from brown algae that, when it comes in contact with calcium chloride (CaCl2), forms a sphere or “bead.” These spheres may then be put into a solution containing substrate to test for enzyme activity. The spheres are easy to manipulate, one doesn’t have the variability and mess of a yeast solution, and since there are no cells in solution, there is nothing to interfere with the various assay methods one might want to use to test for product. The graduated cylinder method for testing catalase activity introduced here is especially good for collecting large amounts of data that enable students to use statistics and, unlike similar yeast catalase experiments using paper disks and a yeast solution, the yeast spheres are easy to manipulate and there is very little variability. I have used this procedure with students in class and with teachers in workshops with positive results and comments.
format	article
author	Pamela J. Bryer
author_facet	Pamela J. Bryer
author_sort	Pamela J. Bryer
title	Exploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)
title_short	Exploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)
title_full	Exploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)
title_fullStr	Exploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)
title_full_unstemmed	Exploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)
title_sort	exploring catalase and invertase activity using sodium alginate-encapsulated yeast (yeast spheres)
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/06883c16b9eb40588109f217d1b72bb1
work_keys_str_mv	AT pamelajbryer exploringcatalaseandinvertaseactivityusingsodiumalginateencapsulatedyeastyeastspheres
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Exploring Catalase and Invertase Activity Using Sodium Alginate-Encapsulated Yeast (Yeast Spheres)

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