Adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis

Uncovering involvement of the purinergic system in the pathogenesis of ventilation disorders (VD) may provide additional information about the pathophysiological mechanisms leading to the development of VD in pulmonary tuberculosis (PT). The aim was to identify a relationship between the parameters...

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Autores principales:	M. E. Dyakova, N. B. Serebryanaya, L. D. Kiryukhina, D. S. Esmedlyaeva, P. K. Yablonskiy
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	purinergic system adenosine adenosinedeaminase tuberculosis ventilation disorders oxygen and nitric oxide radicals Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/068afa27d5964c3c9cf13f61d8522939
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spelling	oai:doaj.org-article:068afa27d5964c3c9cf13f61d85229392021-11-22T07:09:55ZAdenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis2220-76192313-739810.15789/2220-7619-ARM-1475https://doaj.org/article/068afa27d5964c3c9cf13f61d85229392021-09-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1475https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Uncovering involvement of the purinergic system in the pathogenesis of ventilation disorders (VD) may provide additional information about the pathophysiological mechanisms leading to the development of VD in pulmonary tuberculosis (PT). The aim was to identify a relationship between the parameters of adenosine metabolism, inflammatory response and altered ventilation metabolism in PT patients. Materials and methods. Obstructive and mixed PT patients were assigned to subgroups with/without VD for assessing adenosine deaminase activity (ADA-1, 2) in serum, mononuclear cells, neutrophils; ecto-5’-nucleotidase (ecto-5’-NT); CD26 (dipeptidyl peptidase-4, DPP-4), phagocyte oxidative burst measured by NO generation. Results. PT patients showed decreased ADA-1 and CD26 (DPP-4), but increased ADA-2. Elevated intracellular adenosine concentration was found in mononuclear cells in patients lacking VD, whereas patients with mixed and obstructive VD — had it in neutrophils. Mononuclear cells of patients with PT lacking VD as well as with obstructive VD type had decreased NO3– concentration. Neutrophil hyperactivity was recorded in all groups of PT patients. Patients with PT lacking VD as well as with mixed VD type showed that the parameters of external respiration were associated with activity of extra-/intracellular ADA, whereas obstructive VD was caused by excessive formation of serum adenosine. Changes in respiratory function in PT were associated with decreased level of serum NO radicals, impaired nitrogen-dependent bactericidal phagocyte activity, and overproduced neutrophil oxygen radicals. Conclusion. Purinergic regulation is involved in regulating inflammatory and compensatory processes in PT patients as well as impaired ventilation efficiency. The most severe respiratory disorders observed in PT patients with mixed VD type are associated with the most prominent changes in nucleotidase activity, particularly ecto-ADA-2 and DPP-4/CD26.M. E. DyakovaN. B. SerebryanayaL. D. KiryukhinaD. S. EsmedlyaevaP. K. YablonskiySankt-Peterburg : NIIÈM imeni Pasteraarticlepurinergic systemadenosineadenosinedeaminasetuberculosisventilation disordersoxygen and nitric oxide radicalsInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 4, Pp 671-682 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	purinergic system adenosine adenosinedeaminase tuberculosis ventilation disorders oxygen and nitric oxide radicals Infectious and parasitic diseases RC109-216
spellingShingle	purinergic system adenosine adenosinedeaminase tuberculosis ventilation disorders oxygen and nitric oxide radicals Infectious and parasitic diseases RC109-216 M. E. Dyakova N. B. Serebryanaya L. D. Kiryukhina D. S. Esmedlyaeva P. K. Yablonskiy Adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis
description	Uncovering involvement of the purinergic system in the pathogenesis of ventilation disorders (VD) may provide additional information about the pathophysiological mechanisms leading to the development of VD in pulmonary tuberculosis (PT). The aim was to identify a relationship between the parameters of adenosine metabolism, inflammatory response and altered ventilation metabolism in PT patients. Materials and methods. Obstructive and mixed PT patients were assigned to subgroups with/without VD for assessing adenosine deaminase activity (ADA-1, 2) in serum, mononuclear cells, neutrophils; ecto-5’-nucleotidase (ecto-5’-NT); CD26 (dipeptidyl peptidase-4, DPP-4), phagocyte oxidative burst measured by NO generation. Results. PT patients showed decreased ADA-1 and CD26 (DPP-4), but increased ADA-2. Elevated intracellular adenosine concentration was found in mononuclear cells in patients lacking VD, whereas patients with mixed and obstructive VD — had it in neutrophils. Mononuclear cells of patients with PT lacking VD as well as with obstructive VD type had decreased NO3– concentration. Neutrophil hyperactivity was recorded in all groups of PT patients. Patients with PT lacking VD as well as with mixed VD type showed that the parameters of external respiration were associated with activity of extra-/intracellular ADA, whereas obstructive VD was caused by excessive formation of serum adenosine. Changes in respiratory function in PT were associated with decreased level of serum NO radicals, impaired nitrogen-dependent bactericidal phagocyte activity, and overproduced neutrophil oxygen radicals. Conclusion. Purinergic regulation is involved in regulating inflammatory and compensatory processes in PT patients as well as impaired ventilation efficiency. The most severe respiratory disorders observed in PT patients with mixed VD type are associated with the most prominent changes in nucleotidase activity, particularly ecto-ADA-2 and DPP-4/CD26.
format	article
author	M. E. Dyakova N. B. Serebryanaya L. D. Kiryukhina D. S. Esmedlyaeva P. K. Yablonskiy
author_facet	M. E. Dyakova N. B. Serebryanaya L. D. Kiryukhina D. S. Esmedlyaeva P. K. Yablonskiy
author_sort	M. E. Dyakova
title	Adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis
title_short	Adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis
title_full	Adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis
title_fullStr	Adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis
title_full_unstemmed	Adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis
title_sort	adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/068afa27d5964c3c9cf13f61d8522939
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Adenosine-regulated mechanisms in the pathogenesis of ventilation disorders in patients with pulmonary tuberculosis

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