Clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers

Background: It is known that wound healing is Impaired in diabetes mellitus. Possible reasons are widely being searched. However, despite all the available data, reliable markers of reparative processes in diabetes mellitus are needed to be found. Aim: To study morphological and some immunohistoche...

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Autores principales:	Alla Y. Tokmakova, Ekaterina L. Zaitseva, Iya A. Voronkova, Marina V. Shestakova
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2018
Materias:	diabetes mellitus chronic wounds repair immunohistochemical markers histology Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/069b20d47d2f47b5a73b20003b31427e
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spelling	oai:doaj.org-article:069b20d47d2f47b5a73b20003b31427e2021-11-14T09:00:22ZClinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers2072-03512072-037810.14341/DM9823https://doaj.org/article/069b20d47d2f47b5a73b20003b31427e2018-12-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/9823https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378Background: It is known that wound healing is Impaired in diabetes mellitus. Possible reasons are widely being searched. However, despite all the available data, reliable markers of reparative processes in diabetes mellitus are needed to be found. Aim: To study morphological and some immunohistochemical markers of tissue repair in patients with diabetic foot ulcers after local treatment. Materials and methods: 70 patients with diabetic foot ulcers before and after surgical debridement were included. Histological (light microscopy) and immunohistochemical (CD68, MMP-9, TIMP-1) characteristics of tissue repair processes in soft tissues of the lower extremities in patients with diabetes mellitus were analyzed. Histological and immunohistochemical examination of soft tissues were performed in 63 patients before and after surgical debridement and 10 days after local treatment. Results: After the surgical debridement a significant reduction in the area of wounds was registered by 23.4% (p <0.05), wound depth by 29.4% (p <0.05). Based on the results of the morphological study, the presence of mature granulation tissue in the wounds was confirmed. Immunohistochemical study of wound biopsies demonstrated a significant decrease in proteolytic activity in the wound as a decrease in MMP-9 expression (p <0.05). Statistically significant changes in the number of macrophages against the initial data were not found, as well as increased expression of TIMP-1 was observed (p> 0.05 and <0.05, respectively). Conclusion: According to the data, there was a significant decrease in the area and depth of wounds during local treatment. The intensity of tissue repair was confirmed by the results of histological and immunohistochemical studies. However, the absence of a statistically significant change in the amount of macrophages on the background of treatment suggests that this repair link is disrupted in diabetes mellitus, which is the reason for the "chronic" wounds and requires further studies.Alla Y. TokmakovaEkaterina L. ZaitsevaIya A. VoronkovaMarina V. ShestakovaEndocrinology Research Centrearticlediabetes mellituschronic woundsrepairimmunohistochemical markershistologyNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 21, Iss 6, Pp 490-496 (2018)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	diabetes mellitus chronic wounds repair immunohistochemical markers histology Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	diabetes mellitus chronic wounds repair immunohistochemical markers histology Nutritional diseases. Deficiency diseases RC620-627 Alla Y. Tokmakova Ekaterina L. Zaitseva Iya A. Voronkova Marina V. Shestakova Clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers
description	Background: It is known that wound healing is Impaired in diabetes mellitus. Possible reasons are widely being searched. However, despite all the available data, reliable markers of reparative processes in diabetes mellitus are needed to be found. Aim: To study morphological and some immunohistochemical markers of tissue repair in patients with diabetic foot ulcers after local treatment. Materials and methods: 70 patients with diabetic foot ulcers before and after surgical debridement were included. Histological (light microscopy) and immunohistochemical (CD68, MMP-9, TIMP-1) characteristics of tissue repair processes in soft tissues of the lower extremities in patients with diabetes mellitus were analyzed. Histological and immunohistochemical examination of soft tissues were performed in 63 patients before and after surgical debridement and 10 days after local treatment. Results: After the surgical debridement a significant reduction in the area of wounds was registered by 23.4% (p <0.05), wound depth by 29.4% (p <0.05). Based on the results of the morphological study, the presence of mature granulation tissue in the wounds was confirmed. Immunohistochemical study of wound biopsies demonstrated a significant decrease in proteolytic activity in the wound as a decrease in MMP-9 expression (p <0.05). Statistically significant changes in the number of macrophages against the initial data were not found, as well as increased expression of TIMP-1 was observed (p> 0.05 and <0.05, respectively). Conclusion: According to the data, there was a significant decrease in the area and depth of wounds during local treatment. The intensity of tissue repair was confirmed by the results of histological and immunohistochemical studies. However, the absence of a statistically significant change in the amount of macrophages on the background of treatment suggests that this repair link is disrupted in diabetes mellitus, which is the reason for the "chronic" wounds and requires further studies.
format	article
author	Alla Y. Tokmakova Ekaterina L. Zaitseva Iya A. Voronkova Marina V. Shestakova
author_facet	Alla Y. Tokmakova Ekaterina L. Zaitseva Iya A. Voronkova Marina V. Shestakova
author_sort	Alla Y. Tokmakova
title	Clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers
title_short	Clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers
title_full	Clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers
title_fullStr	Clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers
title_full_unstemmed	Clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers
title_sort	clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers
publisher	Endocrinology Research Centre
publishDate	2018
url	https://doaj.org/article/069b20d47d2f47b5a73b20003b31427e
work_keys_str_mv	AT allaytokmakova clinicalpathomorphologicalandimmunohistochemicalevaluationoftissuerepairindiabeticfootulcers AT ekaterinalzaitseva clinicalpathomorphologicalandimmunohistochemicalevaluationoftissuerepairindiabeticfootulcers AT iyaavoronkova clinicalpathomorphologicalandimmunohistochemicalevaluationoftissuerepairindiabeticfootulcers AT marinavshestakova clinicalpathomorphologicalandimmunohistochemicalevaluationoftissuerepairindiabeticfootulcers
_version_	1718429506920775680

Clinical, pathomorphological and immunohistochemical evaluation of tissue repair in diabetic foot ulcers

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