A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules

Abstract Detailed knowledge on the fate of dietary components inside the human intestinal tract is lacking. Access to this inner world of digestion is now possible through novel human gastrointestinal sampling capsules. Due to the novelty of such devices, no methodology has been published to stabili...

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Autores principales:	Melany Rios-Morales, Mara P. H. van Trijp, Christiane Rösch, Ran An, Theo Boer, Albert Gerding, Naomi de Ruiter, Martijn Koehorst, M. Rebecca Heiner-Fokkema, Henk A. Schols, Dirk-Jan Reijngoud, Guido J. E. J. Hooiveld, Barbara M. Bakker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06a21ae74d734b99a00a021edd180d58
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spelling	oai:doaj.org-article:06a21ae74d734b99a00a021edd180d582021-12-02T15:51:16ZA toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules10.1038/s41598-021-86980-y2045-2322https://doaj.org/article/06a21ae74d734b99a00a021edd180d582021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86980-yhttps://doaj.org/toc/2045-2322Abstract Detailed knowledge on the fate of dietary components inside the human intestinal tract is lacking. Access to this inner world of digestion is now possible through novel human gastrointestinal sampling capsules. Due to the novelty of such devices, no methodology has been published to stabilise and analyse the resulting samples. A complicating factor is that excretion of such capsules in faeces may take days, while degradation of the dietary components continues. Therefore a stabilising reagent should be pre-loaded in the capsule to ensure the measurement of a representative sample. Considering the small volume of recovered samples, analytical methods must be optimized to collect as many data as possible from little material. We present a complete workflow for stabilising and analysing the fermentation status of dietary fibres in such samples, including microbiota, fibre degradation, and short chain fatty acids. The final quenching reagent was designed based on safety and effectiveness to inhibit fructo- and galacto-oligosaccharides degradation and short chain fatty acids production by human ileostomy microbiota, and subsequently validated in faecal samples. The final composition of the stock quenching reagent is 175 mM Tris, 525 mM NaCl, 35 mM EDTA, 12% SDS, and 8 M urea at pH 8.5.Melany Rios-MoralesMara P. H. van TrijpChristiane RöschRan AnTheo BoerAlbert GerdingNaomi de RuiterMartijn KoehorstM. Rebecca Heiner-FokkemaHenk A. ScholsDirk-Jan ReijngoudGuido J. E. J. HooiveldBarbara M. BakkerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melany Rios-Morales Mara P. H. van Trijp Christiane Rösch Ran An Theo Boer Albert Gerding Naomi de Ruiter Martijn Koehorst M. Rebecca Heiner-Fokkema Henk A. Schols Dirk-Jan Reijngoud Guido J. E. J. Hooiveld Barbara M. Bakker A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules
description	Abstract Detailed knowledge on the fate of dietary components inside the human intestinal tract is lacking. Access to this inner world of digestion is now possible through novel human gastrointestinal sampling capsules. Due to the novelty of such devices, no methodology has been published to stabilise and analyse the resulting samples. A complicating factor is that excretion of such capsules in faeces may take days, while degradation of the dietary components continues. Therefore a stabilising reagent should be pre-loaded in the capsule to ensure the measurement of a representative sample. Considering the small volume of recovered samples, analytical methods must be optimized to collect as many data as possible from little material. We present a complete workflow for stabilising and analysing the fermentation status of dietary fibres in such samples, including microbiota, fibre degradation, and short chain fatty acids. The final quenching reagent was designed based on safety and effectiveness to inhibit fructo- and galacto-oligosaccharides degradation and short chain fatty acids production by human ileostomy microbiota, and subsequently validated in faecal samples. The final composition of the stock quenching reagent is 175 mM Tris, 525 mM NaCl, 35 mM EDTA, 12% SDS, and 8 M urea at pH 8.5.
format	article
author	Melany Rios-Morales Mara P. H. van Trijp Christiane Rösch Ran An Theo Boer Albert Gerding Naomi de Ruiter Martijn Koehorst M. Rebecca Heiner-Fokkema Henk A. Schols Dirk-Jan Reijngoud Guido J. E. J. Hooiveld Barbara M. Bakker
author_facet	Melany Rios-Morales Mara P. H. van Trijp Christiane Rösch Ran An Theo Boer Albert Gerding Naomi de Ruiter Martijn Koehorst M. Rebecca Heiner-Fokkema Henk A. Schols Dirk-Jan Reijngoud Guido J. E. J. Hooiveld Barbara M. Bakker
author_sort	Melany Rios-Morales
title	A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules
title_short	A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules
title_full	A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules
title_fullStr	A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules
title_full_unstemmed	A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules
title_sort	toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/06a21ae74d734b99a00a021edd180d58
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A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules

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