D-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression

Context: We reported that D-4F, an apolipoprotein A-I (Apo A-I) mimetic polypeptide with 18 d-amino acids, suppressed IL-4 induced macrophage alternative activation and TGF-β1 expression in phorbol 12-myristate 13-acetate (PMA) treated human acute monocytic leukemia cells (THP-1). Objective: Macroph...

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Autores principales:	Xuejiao Song, Ying Shi, Jia You, Zhengshu Wang, Linshen Xie, Chaoxiong Zhang, Jingyuan Xiong
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	apo a-i mimetic thp-1 pulmonary fibrosis monocyte-derived macrophages m2 macrophage Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/06a7d05fb90a4f6d9e6c9d5d021c66e8
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spelling	oai:doaj.org-article:06a7d05fb90a4f6d9e6c9d5d021c66e82021-11-17T14:21:56ZD-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression1388-02091744-511610.1080/13880209.2019.1640747https://doaj.org/article/06a7d05fb90a4f6d9e6c9d5d021c66e82019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1640747https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: We reported that D-4F, an apolipoprotein A-I (Apo A-I) mimetic polypeptide with 18 d-amino acids, suppressed IL-4 induced macrophage alternative activation and TGF-β1 expression in phorbol 12-myristate 13-acetate (PMA) treated human acute monocytic leukemia cells (THP-1). Objective: Macrophage alternative activation, TGF-β1 and epithelial-mesenchymal transition (EMT) are intensively involved in pulmonary fibrosis. Recent studies demonstrated that Apo A-I resolved established pulmonary fibrotic nodules, and D-4F inhibited TGF-β1 induced EMT in alveolar cells. Therefore, this study evaluated the effects of D-4F on IL-4 induced macrophage alternative activation and TGF-β1 expression. Materials and methods: THP-1 cells were simulated with PMA (100 ng/mL) for 48 h and treated with medium control, IL-4 (20 ng/mL) alone, or IL-4 (20 ng/mL) in the presence of D-4F (1, 5, and 10 μg/mL) for 24 and 48 h. Flow cytometry, RT-PCR and ELISA evaluations were performed to investigate the subsequent effects of D-4F. Results: Compared to stimulation with IL-4 alone, 1, 5, and 10 μg/mL of D-4F reduced alternative activation by 45.38%, 59.98%, and 60.10%, increased TNF-α mRNA levels by 8%, 11%, and 16% and decreased TGF-β1 mRNA levels by 21%, 37%, and 39%, respectively (all p ≤ 0.05). In addition, TNF-α protein levels increased from 388 pg/mL (IL-4 alone) to 429, 475, and 487 pg/mL (1, 5, and 10 μg/mL D-4F), while TGF-β1 protein levels dropped from 27.01 pg/mL (IL-4 alone) to 19.15, 12.27, and 10.47 pg/mL (1, 5, and 10 μg/mL D-4F). Conclusion: D-4F suppressed IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression.Xuejiao SongYing ShiJia YouZhengshu WangLinshen XieChaoxiong ZhangJingyuan XiongTaylor & Francis Grouparticleapo a-i mimeticthp-1pulmonary fibrosismonocyte-derived macrophagesm2 macrophageTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 470-476 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	apo a-i mimetic thp-1 pulmonary fibrosis monocyte-derived macrophages m2 macrophage Therapeutics. Pharmacology RM1-950
spellingShingle	apo a-i mimetic thp-1 pulmonary fibrosis monocyte-derived macrophages m2 macrophage Therapeutics. Pharmacology RM1-950 Xuejiao Song Ying Shi Jia You Zhengshu Wang Linshen Xie Chaoxiong Zhang Jingyuan Xiong D-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression
description	Context: We reported that D-4F, an apolipoprotein A-I (Apo A-I) mimetic polypeptide with 18 d-amino acids, suppressed IL-4 induced macrophage alternative activation and TGF-β1 expression in phorbol 12-myristate 13-acetate (PMA) treated human acute monocytic leukemia cells (THP-1). Objective: Macrophage alternative activation, TGF-β1 and epithelial-mesenchymal transition (EMT) are intensively involved in pulmonary fibrosis. Recent studies demonstrated that Apo A-I resolved established pulmonary fibrotic nodules, and D-4F inhibited TGF-β1 induced EMT in alveolar cells. Therefore, this study evaluated the effects of D-4F on IL-4 induced macrophage alternative activation and TGF-β1 expression. Materials and methods: THP-1 cells were simulated with PMA (100 ng/mL) for 48 h and treated with medium control, IL-4 (20 ng/mL) alone, or IL-4 (20 ng/mL) in the presence of D-4F (1, 5, and 10 μg/mL) for 24 and 48 h. Flow cytometry, RT-PCR and ELISA evaluations were performed to investigate the subsequent effects of D-4F. Results: Compared to stimulation with IL-4 alone, 1, 5, and 10 μg/mL of D-4F reduced alternative activation by 45.38%, 59.98%, and 60.10%, increased TNF-α mRNA levels by 8%, 11%, and 16% and decreased TGF-β1 mRNA levels by 21%, 37%, and 39%, respectively (all p ≤ 0.05). In addition, TNF-α protein levels increased from 388 pg/mL (IL-4 alone) to 429, 475, and 487 pg/mL (1, 5, and 10 μg/mL D-4F), while TGF-β1 protein levels dropped from 27.01 pg/mL (IL-4 alone) to 19.15, 12.27, and 10.47 pg/mL (1, 5, and 10 μg/mL D-4F). Conclusion: D-4F suppressed IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression.
format	article
author	Xuejiao Song Ying Shi Jia You Zhengshu Wang Linshen Xie Chaoxiong Zhang Jingyuan Xiong
author_facet	Xuejiao Song Ying Shi Jia You Zhengshu Wang Linshen Xie Chaoxiong Zhang Jingyuan Xiong
author_sort	Xuejiao Song
title	D-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression
title_short	D-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression
title_full	D-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression
title_fullStr	D-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression
title_full_unstemmed	D-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression
title_sort	d-4f, an apolipoprotein a-i mimetic, suppresses il-4 induced macrophage alternative activation and pro-fibrotic tgf-β1 expression
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/06a7d05fb90a4f6d9e6c9d5d021c66e8
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D-4F, an apolipoprotein A-I mimetic, suppresses IL-4 induced macrophage alternative activation and pro-fibrotic TGF-β1 expression

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