Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders

β-Tricalcium Phosphate (β-TCP), one of the most used bone graft substitutes, may contain up to 5 wt% foreign phase according to standards. Typical foreign phases include β-calcium pyrophosphate (β-CPP) and hydroxyapatite (HA). Currently, the effect of small amounts of impurities on β-TCP resorption...

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Autores principales:	B. Le Gars Santoni, L. Niggli, S. Dolder, O. Loeffel, G.A. Sblendorio, R. Heuberger, Y. Maazouz, C. Stähli, N. Döbelin, P. Bowen, W. Hofstetter, M. Bohner
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Bioceramics Calcium phosphate β‐Tricalcium phosphate β‐Calcium pyrophosphate Hydroxyapatite Osteoclastic resorption Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/06a920e8365147209127b8f157decd22
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spelling	oai:doaj.org-article:06a920e8365147209127b8f157decd222021-11-28T04:35:26ZEffect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders2452-199X10.1016/j.bioactmat.2021.09.003https://doaj.org/article/06a920e8365147209127b8f157decd222022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21004138https://doaj.org/toc/2452-199Xβ-Tricalcium Phosphate (β-TCP), one of the most used bone graft substitutes, may contain up to 5 wt% foreign phase according to standards. Typical foreign phases include β-calcium pyrophosphate (β-CPP) and hydroxyapatite (HA). Currently, the effect of small amounts of impurities on β-TCP resorption is unknown. This is surprising since pyrophosphate is a very potent osteoclast inhibitor. The main aim of this study was to assess the effect of small β-CPP fractions (<1 wt%) on the in vitro osteoclastic resorption of β-TCP. A minor aim was to examine the effect of β-CPP and HA impurities on the physico-chemical properties of β-TCP powders and sintered cylinders. Twenty-six batches of β-TCP powder were produced with a Ca/P molar ratio varying between 1.440 and 1.550. Fifteen were further processed to obtain dense and polished β-TCP cylinders. Finally, six of them, with a Ca/P molar ratio varying between 1.496 (1 wt% β-CPP) and 1.502 (1 wt% HA), were incubated in the presence of osteoclasts. Resorption was quantified by white-light interferometry. Osteoclastic resorption was significantly inhibited by β-CPP fraction in a linear manner. The presence of 1% β-CPP reduced β-TCP resorption by 40%, which underlines the importance of controlling β-CPP content when assessing β-TCP biological performance.B. Le Gars SantoniL. NiggliS. DolderO. LoeffelG.A. SblendorioR. HeubergerY. MaazouzC. StähliN. DöbelinP. BowenW. HofstetterM. BohnerKeAi Communications Co., Ltd.articleBioceramicsCalcium phosphateβ‐Tricalcium phosphateβ‐Calcium pyrophosphateHydroxyapatiteOsteoclastic resorptionMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 222-235 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bioceramics Calcium phosphate β‐Tricalcium phosphate β‐Calcium pyrophosphate Hydroxyapatite Osteoclastic resorption Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Bioceramics Calcium phosphate β‐Tricalcium phosphate β‐Calcium pyrophosphate Hydroxyapatite Osteoclastic resorption Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 B. Le Gars Santoni L. Niggli S. Dolder O. Loeffel G.A. Sblendorio R. Heuberger Y. Maazouz C. Stähli N. Döbelin P. Bowen W. Hofstetter M. Bohner Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders
description	β-Tricalcium Phosphate (β-TCP), one of the most used bone graft substitutes, may contain up to 5 wt% foreign phase according to standards. Typical foreign phases include β-calcium pyrophosphate (β-CPP) and hydroxyapatite (HA). Currently, the effect of small amounts of impurities on β-TCP resorption is unknown. This is surprising since pyrophosphate is a very potent osteoclast inhibitor. The main aim of this study was to assess the effect of small β-CPP fractions (<1 wt%) on the in vitro osteoclastic resorption of β-TCP. A minor aim was to examine the effect of β-CPP and HA impurities on the physico-chemical properties of β-TCP powders and sintered cylinders. Twenty-six batches of β-TCP powder were produced with a Ca/P molar ratio varying between 1.440 and 1.550. Fifteen were further processed to obtain dense and polished β-TCP cylinders. Finally, six of them, with a Ca/P molar ratio varying between 1.496 (1 wt% β-CPP) and 1.502 (1 wt% HA), were incubated in the presence of osteoclasts. Resorption was quantified by white-light interferometry. Osteoclastic resorption was significantly inhibited by β-CPP fraction in a linear manner. The presence of 1% β-CPP reduced β-TCP resorption by 40%, which underlines the importance of controlling β-CPP content when assessing β-TCP biological performance.
format	article
author	B. Le Gars Santoni L. Niggli S. Dolder O. Loeffel G.A. Sblendorio R. Heuberger Y. Maazouz C. Stähli N. Döbelin P. Bowen W. Hofstetter M. Bohner
author_facet	B. Le Gars Santoni L. Niggli S. Dolder O. Loeffel G.A. Sblendorio R. Heuberger Y. Maazouz C. Stähli N. Döbelin P. Bowen W. Hofstetter M. Bohner
author_sort	B. Le Gars Santoni
title	Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders
title_short	Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders
title_full	Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders
title_fullStr	Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders
title_full_unstemmed	Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders
title_sort	effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/06a920e8365147209127b8f157decd22
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Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders

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