Persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.

<h4>Introduction</h4>Since persulfate salts are an important cause of occupational asthma (OA), we aimed to study the persistence of respiratory symptoms after a single exposure to ammonium persulfate (AP) in AP-sensitized mice.<h4>Material and methods</h4>BALB/c mice receive...

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Autores principales:	Marta Ollé-Monge, Xavier Muñoz, Jeroen A J Vanoirbeek, Susana Gómez-Ollés, Ferran Morell, María-Jesus Cruz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06b5980e04144e3eaea09b89e6b01ead
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spelling	oai:doaj.org-article:06b5980e04144e3eaea09b89e6b01ead2021-11-25T05:57:03ZPersistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.1932-620310.1371/journal.pone.0109000https://doaj.org/article/06b5980e04144e3eaea09b89e6b01ead2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109000https://doaj.org/toc/1932-6203<h4>Introduction</h4>Since persulfate salts are an important cause of occupational asthma (OA), we aimed to study the persistence of respiratory symptoms after a single exposure to ammonium persulfate (AP) in AP-sensitized mice.<h4>Material and methods</h4>BALB/c mice received dermal applications of AP or dimethylsulfoxide (DMSO) on days 1 and 8. On day 15, they received a single nasal instillation of AP or saline. Airway hyperresponsiveness (AHR) was assessed using methacholine provocation, while pulmonary inflammation was evaluated in bronchoalveolar lavage (BAL), and total serum immunoglobulin E (IgE), IgG1 and IgG2a were measured in blood at 1, 4, 8, 24 hours and 4, 8, 15 days after the single exposure to the causal agent. Histological studies of lungs were assessed.<h4>Results</h4>AP-treated mice showed a sustained increase in AHR, lasting up to 4 days after the challenge. There was a significant increase in the percentage of neutrophils 8 hours after the challenge, which persisted for 24 hours in AP-treated mice. The extent of airway inflammation was also seen in the histological analysis of the lungs from challenged mice. Slight increases in total serum IgE 4 days after the challenge were found, while IgG gradually increased further 4 to 15 days after the AP challenge in AP-sensitized mice.<h4>Conclusions</h4>In AP-sensitized mice, an Ig-independent response is induced after AP challenge. AHR appears immediately, but airway neutrophil inflammation appears later. This response decreases in time; at early stages only respiratory and inflammatory responses decrease, but later on immunological response decreases as well.Marta Ollé-MongeXavier MuñozJeroen A J VanoirbeekSusana Gómez-OllésFerran MorellMaría-Jesus CruzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109000 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Ollé-Monge Xavier Muñoz Jeroen A J Vanoirbeek Susana Gómez-Ollés Ferran Morell María-Jesus Cruz Persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.
description	<h4>Introduction</h4>Since persulfate salts are an important cause of occupational asthma (OA), we aimed to study the persistence of respiratory symptoms after a single exposure to ammonium persulfate (AP) in AP-sensitized mice.<h4>Material and methods</h4>BALB/c mice received dermal applications of AP or dimethylsulfoxide (DMSO) on days 1 and 8. On day 15, they received a single nasal instillation of AP or saline. Airway hyperresponsiveness (AHR) was assessed using methacholine provocation, while pulmonary inflammation was evaluated in bronchoalveolar lavage (BAL), and total serum immunoglobulin E (IgE), IgG1 and IgG2a were measured in blood at 1, 4, 8, 24 hours and 4, 8, 15 days after the single exposure to the causal agent. Histological studies of lungs were assessed.<h4>Results</h4>AP-treated mice showed a sustained increase in AHR, lasting up to 4 days after the challenge. There was a significant increase in the percentage of neutrophils 8 hours after the challenge, which persisted for 24 hours in AP-treated mice. The extent of airway inflammation was also seen in the histological analysis of the lungs from challenged mice. Slight increases in total serum IgE 4 days after the challenge were found, while IgG gradually increased further 4 to 15 days after the AP challenge in AP-sensitized mice.<h4>Conclusions</h4>In AP-sensitized mice, an Ig-independent response is induced after AP challenge. AHR appears immediately, but airway neutrophil inflammation appears later. This response decreases in time; at early stages only respiratory and inflammatory responses decrease, but later on immunological response decreases as well.
format	article
author	Marta Ollé-Monge Xavier Muñoz Jeroen A J Vanoirbeek Susana Gómez-Ollés Ferran Morell María-Jesus Cruz
author_facet	Marta Ollé-Monge Xavier Muñoz Jeroen A J Vanoirbeek Susana Gómez-Ollés Ferran Morell María-Jesus Cruz
author_sort	Marta Ollé-Monge
title	Persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.
title_short	Persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.
title_full	Persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.
title_fullStr	Persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.
title_full_unstemmed	Persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.
title_sort	persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/06b5980e04144e3eaea09b89e6b01ead
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Persistence of asthmatic response after ammonium persulfate-induced occupational asthma in mice.

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