TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis

Transient receptor potential melastatin 7 (TRPM7) contributes to a variety of physiological and pathological processes in many tissues and cells. With a widespread distribution in the nervous system, TRPM7 is involved in animal behaviors and neuronal death induced by ischemia. However, the physiolog...

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Autores principales:	Zhong-Jiao Jiang, Wenping Li, Li-Hua Yao, Badeia Saed, Yan Rao, Brian S Grewe, Andrea McGinley, Kelly Varga, Simon Alford, Ying S Hu, Liang-Wei Gong
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	synaptic vesicle endocytosis short-term synaptic depression cell-attached capacitance recordings SyGCaMP6f iGluSnFR pHluorin Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/06beb57d36444a1a84531c47103de50b
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spelling	oai:doaj.org-article:06beb57d36444a1a84531c47103de50b2021-11-15T06:05:31ZTRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis10.7554/eLife.667092050-084Xe66709https://doaj.org/article/06beb57d36444a1a84531c47103de50b2021-09-01T00:00:00Zhttps://elifesciences.org/articles/66709https://doaj.org/toc/2050-084XTransient receptor potential melastatin 7 (TRPM7) contributes to a variety of physiological and pathological processes in many tissues and cells. With a widespread distribution in the nervous system, TRPM7 is involved in animal behaviors and neuronal death induced by ischemia. However, the physiological role of TRPM7 in central nervous system (CNS) neuron remains unclear. Here, we identify endocytic defects in neuroendocrine cells and neurons from TRPM7 knockout (KO) mice, indicating a role of TRPM7 in synaptic vesicle endocytosis. Our experiments further pinpoint the importance of TRPM7 as an ion channel in synaptic vesicle endocytosis. Ca2+ imaging detects a defect in presynaptic Ca2+ dynamics in TRPM7 KO neuron, suggesting an importance of Ca2+ influx via TRPM7 in synaptic vesicle endocytosis. Moreover, the short-term depression is enhanced in both excitatory and inhibitory synaptic transmissions from TRPM7 KO mice. Taken together, our data suggests that Ca2+ influx via TRPM7 may be critical for short-term plasticity of synaptic strength by regulating synaptic vesicle endocytosis in neurons.Zhong-Jiao JiangWenping LiLi-Hua YaoBadeia SaedYan RaoBrian S GreweAndrea McGinleyKelly VargaSimon AlfordYing S HuLiang-Wei GongeLife Sciences Publications Ltdarticlesynaptic vesicle endocytosisshort-term synaptic depressioncell-attached capacitance recordingsSyGCaMP6fiGluSnFRpHluorinMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	synaptic vesicle endocytosis short-term synaptic depression cell-attached capacitance recordings SyGCaMP6f iGluSnFR pHluorin Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	synaptic vesicle endocytosis short-term synaptic depression cell-attached capacitance recordings SyGCaMP6f iGluSnFR pHluorin Medicine R Science Q Biology (General) QH301-705.5 Zhong-Jiao Jiang Wenping Li Li-Hua Yao Badeia Saed Yan Rao Brian S Grewe Andrea McGinley Kelly Varga Simon Alford Ying S Hu Liang-Wei Gong TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis
description	Transient receptor potential melastatin 7 (TRPM7) contributes to a variety of physiological and pathological processes in many tissues and cells. With a widespread distribution in the nervous system, TRPM7 is involved in animal behaviors and neuronal death induced by ischemia. However, the physiological role of TRPM7 in central nervous system (CNS) neuron remains unclear. Here, we identify endocytic defects in neuroendocrine cells and neurons from TRPM7 knockout (KO) mice, indicating a role of TRPM7 in synaptic vesicle endocytosis. Our experiments further pinpoint the importance of TRPM7 as an ion channel in synaptic vesicle endocytosis. Ca2+ imaging detects a defect in presynaptic Ca2+ dynamics in TRPM7 KO neuron, suggesting an importance of Ca2+ influx via TRPM7 in synaptic vesicle endocytosis. Moreover, the short-term depression is enhanced in both excitatory and inhibitory synaptic transmissions from TRPM7 KO mice. Taken together, our data suggests that Ca2+ influx via TRPM7 may be critical for short-term plasticity of synaptic strength by regulating synaptic vesicle endocytosis in neurons.
format	article
author	Zhong-Jiao Jiang Wenping Li Li-Hua Yao Badeia Saed Yan Rao Brian S Grewe Andrea McGinley Kelly Varga Simon Alford Ying S Hu Liang-Wei Gong
author_facet	Zhong-Jiao Jiang Wenping Li Li-Hua Yao Badeia Saed Yan Rao Brian S Grewe Andrea McGinley Kelly Varga Simon Alford Ying S Hu Liang-Wei Gong
author_sort	Zhong-Jiao Jiang
title	TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis
title_short	TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis
title_full	TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis
title_fullStr	TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis
title_full_unstemmed	TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis
title_sort	trpm7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/06beb57d36444a1a84531c47103de50b
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TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis

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