Laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor

Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tuberculosis), requires a high level of attention and is one of the most infectious diseases in the air. Present methods of diagnosing TB remain ineffective owing to their low sensitivity and time consumption. In this study, we pro...

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Autores principales:	Melvin Jia Yong Tai, Veeradasan Perumal, Subash C. B. Gopinath, Pandian Bothi Raja, Mohamad Nasir Mohamad Ibrahim, Iffah Najihah Jantan, Nur Syahirah Husna Suhaimi, Wei-Wen Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06c2e48300b84d2aa61375abe0446db8
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spelling	oai:doaj.org-article:06c2e48300b84d2aa61375abe0446db82021-12-02T15:54:02ZLaser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor10.1038/s41598-021-85039-22045-2322https://doaj.org/article/06c2e48300b84d2aa61375abe0446db82021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85039-2https://doaj.org/toc/2045-2322Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tuberculosis), requires a high level of attention and is one of the most infectious diseases in the air. Present methods of diagnosing TB remain ineffective owing to their low sensitivity and time consumption. In this study, we produced a green graphene nanofiber laser biosensor (LSG-NF) decorated with oil palm lignin-based synthetic silver nanoparticles (AgNPs). The resulting composite morphology was observed by field-emission scanning electron microscopy and transmission electron microscopy, which revealed the effective adaptation of the AgNPs to the LSG-NF surface. The successful attachment of AgNPs and LSG-NFs was also evident from X-ray diffraction and Raman spectroscopy studies. In order to verify the sensing efficiency, a selective DNA sample captured on AgNPs was investigated for specific binding with M.tb target DNA through selective hybridisation and mismatch analysis. Electrochemical impedance studies further confirmed sensitive detection of up to 1 fM, where a detection limit of 10−15 M was obtained by estimating the signal-to-noise ratio (S/N = 3:1) as 3σ. Successful DNA immobilisation and hybridisation was confirmed by the detection of phosphorus and nitrogen peaks based on X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy. The stability and repeatability of the analysis were high. This approach provides an affordable potential sensing system for the determination of M. tuberculosis biomarker and thus provides a new direction in medical diagnosis.Melvin Jia Yong TaiVeeradasan PerumalSubash C. B. GopinathPandian Bothi RajaMohamad Nasir Mohamad IbrahimIffah Najihah JantanNur Syahirah Husna SuhaimiWei-Wen LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melvin Jia Yong Tai Veeradasan Perumal Subash C. B. Gopinath Pandian Bothi Raja Mohamad Nasir Mohamad Ibrahim Iffah Najihah Jantan Nur Syahirah Husna Suhaimi Wei-Wen Liu Laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor
description	Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tuberculosis), requires a high level of attention and is one of the most infectious diseases in the air. Present methods of diagnosing TB remain ineffective owing to their low sensitivity and time consumption. In this study, we produced a green graphene nanofiber laser biosensor (LSG-NF) decorated with oil palm lignin-based synthetic silver nanoparticles (AgNPs). The resulting composite morphology was observed by field-emission scanning electron microscopy and transmission electron microscopy, which revealed the effective adaptation of the AgNPs to the LSG-NF surface. The successful attachment of AgNPs and LSG-NFs was also evident from X-ray diffraction and Raman spectroscopy studies. In order to verify the sensing efficiency, a selective DNA sample captured on AgNPs was investigated for specific binding with M.tb target DNA through selective hybridisation and mismatch analysis. Electrochemical impedance studies further confirmed sensitive detection of up to 1 fM, where a detection limit of 10−15 M was obtained by estimating the signal-to-noise ratio (S/N = 3:1) as 3σ. Successful DNA immobilisation and hybridisation was confirmed by the detection of phosphorus and nitrogen peaks based on X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy. The stability and repeatability of the analysis were high. This approach provides an affordable potential sensing system for the determination of M. tuberculosis biomarker and thus provides a new direction in medical diagnosis.
format	article
author	Melvin Jia Yong Tai Veeradasan Perumal Subash C. B. Gopinath Pandian Bothi Raja Mohamad Nasir Mohamad Ibrahim Iffah Najihah Jantan Nur Syahirah Husna Suhaimi Wei-Wen Liu
author_facet	Melvin Jia Yong Tai Veeradasan Perumal Subash C. B. Gopinath Pandian Bothi Raja Mohamad Nasir Mohamad Ibrahim Iffah Najihah Jantan Nur Syahirah Husna Suhaimi Wei-Wen Liu
author_sort	Melvin Jia Yong Tai
title	Laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor
title_short	Laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor
title_full	Laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor
title_fullStr	Laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor
title_full_unstemmed	Laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor
title_sort	laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/06c2e48300b84d2aa61375abe0446db8
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Laser-scribed graphene nanofiber decorated with oil palm lignin capped silver nanoparticles: a green biosensor

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