Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells

Abstract Fusaric acid (FA), a food-borne mycotoxin, is a potent divalent metal chelator. The human immune system is complex and susceptible to environmental insult however, the immunotoxity of FA remains unknown. We investigated the immunotoxicity of FA on human peripheral blood mononuclear cells (P...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shanel Dhani, Savania Nagiah, Dhaneshree B. Naidoo, Anil A. Chuturgoon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06c39d183cc1470a98a5a2a5e327538d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:06c39d183cc1470a98a5a2a5e327538d
record_format	dspace
spelling	oai:doaj.org-article:06c39d183cc1470a98a5a2a5e327538d2021-12-02T11:53:06ZFusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells10.1038/s41598-017-03183-02045-2322https://doaj.org/article/06c39d183cc1470a98a5a2a5e327538d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03183-0https://doaj.org/toc/2045-2322Abstract Fusaric acid (FA), a food-borne mycotoxin, is a potent divalent metal chelator. The human immune system is complex and susceptible to environmental insult however, the immunotoxity of FA remains unknown. We investigated the immunotoxicity of FA on human peripheral blood mononuclear cells (PBMCs) and Thp-1 cells. FA was cytotoxic to PBMCs (IC50-240.8 μg/ml) and Thp-1 (IC50-107.7 μg/ml) cells at 24 h. FA induced early apoptosis but significantly decreased caspase activity in PBMCs, a characteristic of paraptosis. In Thp-1 cells, FA induced apoptosis and increased caspase −9 and −3/7 activities. In PBMCs, FA maintained mitochondrial membrane potential and decreased protein expression of Bax whilst increasing expression of p-Bcl-2; FA induced oxidative stress and depleted ATP levels in both cell types. In Thp-1 cells, FA increased mitochondrial membrane depolarization and decreased p-Bcl-2 expression. In PBMCs, FA significantly up-regulated the MAPK protein expression of p-ERK and p-JNK but down-regulated p-p38 expression. In Thp-1 cells, FA up-regulated MAPK protein expression of p-ERK whilst p-JNK and p-p38 expression were down-regulated. In conclusion FA induced programmed cell death and altered MAPK signaling in healthy PBMCs and Thp-1 cells strongly suggesting a possible mechanism of FA induced immunotoxicity in vitro.Shanel DhaniSavania NagiahDhaneshree B. NaidooAnil A. ChuturgoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shanel Dhani Savania Nagiah Dhaneshree B. Naidoo Anil A. Chuturgoon Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells
description	Abstract Fusaric acid (FA), a food-borne mycotoxin, is a potent divalent metal chelator. The human immune system is complex and susceptible to environmental insult however, the immunotoxity of FA remains unknown. We investigated the immunotoxicity of FA on human peripheral blood mononuclear cells (PBMCs) and Thp-1 cells. FA was cytotoxic to PBMCs (IC50-240.8 μg/ml) and Thp-1 (IC50-107.7 μg/ml) cells at 24 h. FA induced early apoptosis but significantly decreased caspase activity in PBMCs, a characteristic of paraptosis. In Thp-1 cells, FA induced apoptosis and increased caspase −9 and −3/7 activities. In PBMCs, FA maintained mitochondrial membrane potential and decreased protein expression of Bax whilst increasing expression of p-Bcl-2; FA induced oxidative stress and depleted ATP levels in both cell types. In Thp-1 cells, FA increased mitochondrial membrane depolarization and decreased p-Bcl-2 expression. In PBMCs, FA significantly up-regulated the MAPK protein expression of p-ERK and p-JNK but down-regulated p-p38 expression. In Thp-1 cells, FA up-regulated MAPK protein expression of p-ERK whilst p-JNK and p-p38 expression were down-regulated. In conclusion FA induced programmed cell death and altered MAPK signaling in healthy PBMCs and Thp-1 cells strongly suggesting a possible mechanism of FA induced immunotoxicity in vitro.
format	article
author	Shanel Dhani Savania Nagiah Dhaneshree B. Naidoo Anil A. Chuturgoon
author_facet	Shanel Dhani Savania Nagiah Dhaneshree B. Naidoo Anil A. Chuturgoon
author_sort	Shanel Dhani
title	Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells
title_short	Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells
title_full	Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells
title_fullStr	Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells
title_full_unstemmed	Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells
title_sort	fusaric acid immunotoxicity and mapk activation in normal peripheral blood mononuclear cells and thp-1 cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/06c39d183cc1470a98a5a2a5e327538d
work_keys_str_mv	AT shaneldhani fusaricacidimmunotoxicityandmapkactivationinnormalperipheralbloodmononuclearcellsandthp1cells AT savanianagiah fusaricacidimmunotoxicityandmapkactivationinnormalperipheralbloodmononuclearcellsandthp1cells AT dhaneshreebnaidoo fusaricacidimmunotoxicityandmapkactivationinnormalperipheralbloodmononuclearcellsandthp1cells AT anilachuturgoon fusaricacidimmunotoxicityandmapkactivationinnormalperipheralbloodmononuclearcellsandthp1cells
_version_	1718394847597953024

Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells

Ejemplares similares