Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles

Dhananjayan Dhanasooraj, R Ajay Kumar, Sathish MundayoorMycobacterium Research Group, Rajiv Gandhi Centre for Biotechnology, Kerala, IndiaAbstract: Nano-sized hepatitis B virus core virus-like particles (HBc-VLP) are suitable for uptake by antigen-presenting cells. Mycobacterium tuberculosis antigen...

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Autores principales:	Dhanasooraj D, Kumar RA, Mundayoor S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/06c6cd7b7b4f446a89cf34ce5a8225af
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spelling	oai:doaj.org-article:06c6cd7b7b4f446a89cf34ce5a8225af2021-12-02T07:21:42ZVaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles1176-91141178-2013https://doaj.org/article/06c6cd7b7b4f446a89cf34ce5a8225af2013-02-01T00:00:00Zhttp://www.dovepress.com/vaccine-delivery-system-for-tuberculosis-based-on-nano-sized-hepatitis-a12295https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Dhananjayan Dhanasooraj, R Ajay Kumar, Sathish MundayoorMycobacterium Research Group, Rajiv Gandhi Centre for Biotechnology, Kerala, IndiaAbstract: Nano-sized hepatitis B virus core virus-like particles (HBc-VLP) are suitable for uptake by antigen-presenting cells. Mycobacterium tuberculosis antigen culture filtrate protein 10 (CFP-10) is an important vaccine candidate against tuberculosis. The purified antigen shows low immune response without adjuvant and tends to have low protective efficacy. The present study is based on the assumption that expression of these proteins on HBc nanoparticles would provide higher protection when compared to the native antigen alone. The cfp-10 gene was expressed as a fusion on the major immunodominant region of HBc-VLP, and the immune response in Balb/c mice was studied and compared to pure proteins, a mixture of antigens, and fusion protein-VLP, all without using any adjuvant. The humoral, cytokine, and splenocyte cell proliferation responses suggested that the HBc-VLP bearing CFP-10 generated an antigen-specific immune response in a Th1-dependent manner. By virtue of its self-adjuvant nature and ability to form nano-sized particles, HBc-VLPs are an excellent vaccine delivery system for use with subunit protein antigens identified in the course of recent vaccine research.Keywords: Mycobacterium tuberculosis, VLP, hepatitis B virus core particle, CFP-10, self-adjuvant, vaccine deliveryDhanasooraj DKumar RAMundayoor SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 835-843 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Dhanasooraj D Kumar RA Mundayoor S Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles
description	Dhananjayan Dhanasooraj, R Ajay Kumar, Sathish MundayoorMycobacterium Research Group, Rajiv Gandhi Centre for Biotechnology, Kerala, IndiaAbstract: Nano-sized hepatitis B virus core virus-like particles (HBc-VLP) are suitable for uptake by antigen-presenting cells. Mycobacterium tuberculosis antigen culture filtrate protein 10 (CFP-10) is an important vaccine candidate against tuberculosis. The purified antigen shows low immune response without adjuvant and tends to have low protective efficacy. The present study is based on the assumption that expression of these proteins on HBc nanoparticles would provide higher protection when compared to the native antigen alone. The cfp-10 gene was expressed as a fusion on the major immunodominant region of HBc-VLP, and the immune response in Balb/c mice was studied and compared to pure proteins, a mixture of antigens, and fusion protein-VLP, all without using any adjuvant. The humoral, cytokine, and splenocyte cell proliferation responses suggested that the HBc-VLP bearing CFP-10 generated an antigen-specific immune response in a Th1-dependent manner. By virtue of its self-adjuvant nature and ability to form nano-sized particles, HBc-VLPs are an excellent vaccine delivery system for use with subunit protein antigens identified in the course of recent vaccine research.Keywords: Mycobacterium tuberculosis, VLP, hepatitis B virus core particle, CFP-10, self-adjuvant, vaccine delivery
format	article
author	Dhanasooraj D Kumar RA Mundayoor S
author_facet	Dhanasooraj D Kumar RA Mundayoor S
author_sort	Dhanasooraj D
title	Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles
title_short	Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles
title_full	Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles
title_fullStr	Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles
title_full_unstemmed	Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles
title_sort	vaccine delivery system for tuberculosis based on nano-sized hepatitis b virus core protein particles
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/06c6cd7b7b4f446a89cf34ce5a8225af
work_keys_str_mv	AT dhanasoorajd vaccinedeliverysystemfortuberculosisbasedonnanosizedhepatitisbviruscoreproteinparticles AT kumarra vaccinedeliverysystemfortuberculosisbasedonnanosizedhepatitisbviruscoreproteinparticles AT mundayoors vaccinedeliverysystemfortuberculosisbasedonnanosizedhepatitisbviruscoreproteinparticles
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Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles

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