Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a severe syndrome lacking efficient therapy and resulting in high morbidity and mortality. Although resveratrol (RES), a natural phytoalexin, has been reported to protect the ALI by suppressing the inflammatory response, the detai...

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Autores principales:	Guo Bingnan, Peng Yigen, Gu Yuting, Zhong Yi, Su Chenglei, Liu Lin, Chai Dafei, Song Tengfei, Zhao Ningjun, Yan Xianliang, Xu Tie
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	resveratrol lps acute lung injury conventional dendritic cells Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/06c8703fa96745fda282f063da7e6940
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spelling	oai:doaj.org-article:06c8703fa96745fda282f063da7e69402021-12-05T14:10:42ZResveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function2391-541210.1515/biol-2021-0110https://doaj.org/article/06c8703fa96745fda282f063da7e69402021-09-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0110https://doaj.org/toc/2391-5412Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a severe syndrome lacking efficient therapy and resulting in high morbidity and mortality. Although resveratrol (RES), a natural phytoalexin, has been reported to protect the ALI by suppressing the inflammatory response, the detailed mechanism of how RES affected the immune system is poorly studied. Pulmonary conventional dendritic cells (cDCs) are critically involved in the pathogenesis of inflammatory lung diseases including ALI. In this study, we aimed to investigate the protective role of RES via pulmonary cDCs in lipopolysaccharide (LPS)-induced ALI mice. Murine ALI model was established by intratracheally challenging with 5 mg/kg LPS. We found that RES pretreatment could mitigate LPS-induced ALI. Additionally, proinflammatory-skewed cytokines decreased whereas anti-inflammatory-related cytokines increased in bronchoalveolar lavage fluid by RES pretreatment. Mechanistically, RES regulated pulmonary cDCs’ maturation and function, exhibiting lower level of CD80, CD86, major histocompatibility complex (MHC) II expression, and IL-10 secretion in ALI mice. Furthermore, RES modulated the balance between proinflammation and anti-inflammation of cDCs. Moreover, in vitro RES pretreatment regulated the maturation and function of bone marrow derived dendritic cells (BMDCs). Finally, the adoptive transfer of RES-pretreated BMDCs enhanced recovery of ALI. Thus, these data might further extend our understanding of a protective role of RES in regulating pulmonary cDCs against ALI.Guo BingnanPeng YigenGu YutingZhong YiSu ChengleiLiu LinChai DafeiSong TengfeiZhao NingjunYan XianliangXu TieDe Gruyterarticleresveratrollpsacute lung injuryconventional dendritic cellsBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1064-1081 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	resveratrol lps acute lung injury conventional dendritic cells Biology (General) QH301-705.5
spellingShingle	resveratrol lps acute lung injury conventional dendritic cells Biology (General) QH301-705.5 Guo Bingnan Peng Yigen Gu Yuting Zhong Yi Su Chenglei Liu Lin Chai Dafei Song Tengfei Zhao Ningjun Yan Xianliang Xu Tie Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
description	Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a severe syndrome lacking efficient therapy and resulting in high morbidity and mortality. Although resveratrol (RES), a natural phytoalexin, has been reported to protect the ALI by suppressing the inflammatory response, the detailed mechanism of how RES affected the immune system is poorly studied. Pulmonary conventional dendritic cells (cDCs) are critically involved in the pathogenesis of inflammatory lung diseases including ALI. In this study, we aimed to investigate the protective role of RES via pulmonary cDCs in lipopolysaccharide (LPS)-induced ALI mice. Murine ALI model was established by intratracheally challenging with 5 mg/kg LPS. We found that RES pretreatment could mitigate LPS-induced ALI. Additionally, proinflammatory-skewed cytokines decreased whereas anti-inflammatory-related cytokines increased in bronchoalveolar lavage fluid by RES pretreatment. Mechanistically, RES regulated pulmonary cDCs’ maturation and function, exhibiting lower level of CD80, CD86, major histocompatibility complex (MHC) II expression, and IL-10 secretion in ALI mice. Furthermore, RES modulated the balance between proinflammation and anti-inflammation of cDCs. Moreover, in vitro RES pretreatment regulated the maturation and function of bone marrow derived dendritic cells (BMDCs). Finally, the adoptive transfer of RES-pretreated BMDCs enhanced recovery of ALI. Thus, these data might further extend our understanding of a protective role of RES in regulating pulmonary cDCs against ALI.
format	article
author	Guo Bingnan Peng Yigen Gu Yuting Zhong Yi Su Chenglei Liu Lin Chai Dafei Song Tengfei Zhao Ningjun Yan Xianliang Xu Tie
author_facet	Guo Bingnan Peng Yigen Gu Yuting Zhong Yi Su Chenglei Liu Lin Chai Dafei Song Tengfei Zhao Ningjun Yan Xianliang Xu Tie
author_sort	Guo Bingnan
title	Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
title_short	Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
title_full	Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
title_fullStr	Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
title_full_unstemmed	Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
title_sort	resveratrol pretreatment mitigates lps-induced acute lung injury by regulating conventional dendritic cells’ maturation and function
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/06c8703fa96745fda282f063da7e6940
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Resveratrol pretreatment mitigates LPS-induced acute lung injury by regulating conventional dendritic cells’ maturation and function

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