The first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa

Abstract The Eurasian plant Stipa capillata is the most widespread species within feather grasses. Many taxa of the genus are dominants in steppe plant communities and can be used for their classification and in studies related to climate change. Moreover, some species are of economic importance mai...

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Autores principales:	Evgenii Baiakhmetov, Cervin Guyomar, Ekaterina Shelest, Marcin Nobis, Polina D. Gudkova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06cc5f07aa4146d488cdca7c89f6a86a
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spelling	oai:doaj.org-article:06cc5f07aa4146d488cdca7c89f6a86a2021-12-02T16:31:48ZThe first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa10.1038/s41598-021-94068-w2045-2322https://doaj.org/article/06cc5f07aa4146d488cdca7c89f6a86a2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94068-whttps://doaj.org/toc/2045-2322Abstract The Eurasian plant Stipa capillata is the most widespread species within feather grasses. Many taxa of the genus are dominants in steppe plant communities and can be used for their classification and in studies related to climate change. Moreover, some species are of economic importance mainly as fodder plants and can be used for soil remediation processes. Although large-scale molecular data has begun to appear, there is still no complete or draft genome for any Stipa species. Thus, here we present a single-molecule long-read sequencing dataset generated using the Pacific Biosciences Sequel System. A draft genome of about 1004 Mb was obtained with a contig N50 length of 351 kb. Importantly, here we report 81,224 annotated protein-coding genes, present 77,614 perfect and 58 unique imperfect SSRs, reveal the putative allopolyploid nature of S. capillata, investigate the evolutionary history of the genus, demonstrate structural heteroplasmy of the chloroplast genome and announce for the first time the mitochondrial genome in Stipa. The assembled nuclear, mitochondrial and chloroplast genomes provide a significant source of genetic data for further works on phylogeny, hybridisation and population studies within Stipa and the grass family Poaceae.Evgenii BaiakhmetovCervin GuyomarEkaterina ShelestMarcin NobisPolina D. GudkovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evgenii Baiakhmetov Cervin Guyomar Ekaterina Shelest Marcin Nobis Polina D. Gudkova The first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa
description	Abstract The Eurasian plant Stipa capillata is the most widespread species within feather grasses. Many taxa of the genus are dominants in steppe plant communities and can be used for their classification and in studies related to climate change. Moreover, some species are of economic importance mainly as fodder plants and can be used for soil remediation processes. Although large-scale molecular data has begun to appear, there is still no complete or draft genome for any Stipa species. Thus, here we present a single-molecule long-read sequencing dataset generated using the Pacific Biosciences Sequel System. A draft genome of about 1004 Mb was obtained with a contig N50 length of 351 kb. Importantly, here we report 81,224 annotated protein-coding genes, present 77,614 perfect and 58 unique imperfect SSRs, reveal the putative allopolyploid nature of S. capillata, investigate the evolutionary history of the genus, demonstrate structural heteroplasmy of the chloroplast genome and announce for the first time the mitochondrial genome in Stipa. The assembled nuclear, mitochondrial and chloroplast genomes provide a significant source of genetic data for further works on phylogeny, hybridisation and population studies within Stipa and the grass family Poaceae.
format	article
author	Evgenii Baiakhmetov Cervin Guyomar Ekaterina Shelest Marcin Nobis Polina D. Gudkova
author_facet	Evgenii Baiakhmetov Cervin Guyomar Ekaterina Shelest Marcin Nobis Polina D. Gudkova
author_sort	Evgenii Baiakhmetov
title	The first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa
title_short	The first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa
title_full	The first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa
title_fullStr	The first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa
title_full_unstemmed	The first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa
title_sort	first draft genome of feather grasses using smrt sequencing and its implications in molecular studies of stipa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/06cc5f07aa4146d488cdca7c89f6a86a
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The first draft genome of feather grasses using SMRT sequencing and its implications in molecular studies of Stipa

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