Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.

<h4>Background</h4>Arabidopsis plants accumulate maltose from starch breakdown during cold acclimation. The Arabidopsis mutant, maltose excess1-1, accumulates large amounts of maltose in the plastid even in the warm, due to a deficient plastid envelope maltose transporter. We therefore i...

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Autores principales:	Sarah J Purdy, John D Bussell, Christopher P Nunn, Steven M Smith
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06d54dc6236841bfac8856d26a37b10f
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spelling	oai:doaj.org-article:06d54dc6236841bfac8856d26a37b10f2021-11-18T08:48:17ZLeaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.1932-620310.1371/journal.pone.0079412https://doaj.org/article/06d54dc6236841bfac8856d26a37b10f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24223944/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Arabidopsis plants accumulate maltose from starch breakdown during cold acclimation. The Arabidopsis mutant, maltose excess1-1, accumulates large amounts of maltose in the plastid even in the warm, due to a deficient plastid envelope maltose transporter. We therefore investigated whether the elevated maltose level in mex1-1 in the warm could result in changes in metabolism and physiology typical of WT plants grown in the cold.<h4>Principal findings</h4>Grown at 21 °C, mex1-1 plants were much smaller, with fewer leaves, and elevated carbohydrates and amino acids compared to WT. However, after transfer to 4 °C the total soluble sugar pool and amino acid concentration was in equal abundance in both genotypes, although the most abundant sugar in mex1-1 was still maltose whereas sucrose was in greatest abundance in WT. The chlorophyll a/b ratio in WT was much lower in the cold than in the warm, but in mex1-1 it was low in both warm and cold. After prolonged growth at 4 °C, the shoot biomass, rosette diameter and number of leaves at bolting were similar in mex1-1 and WT.<h4>Conclusions</h4>The mex1-1 mutation in warm-grown plants confers aspects of cold acclimation, including elevated levels of sugars and amino acids and low chlorophyll a/b ratio. This may in turn compromise growth of mex1-1 in the warm relative to WT. We suggest that elevated maltose in the plastid could be responsible for key aspects of cold acclimation.Sarah J PurdyJohn D BussellChristopher P NunnSteven M SmithPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79412 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah J Purdy John D Bussell Christopher P Nunn Steven M Smith Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.
description	<h4>Background</h4>Arabidopsis plants accumulate maltose from starch breakdown during cold acclimation. The Arabidopsis mutant, maltose excess1-1, accumulates large amounts of maltose in the plastid even in the warm, due to a deficient plastid envelope maltose transporter. We therefore investigated whether the elevated maltose level in mex1-1 in the warm could result in changes in metabolism and physiology typical of WT plants grown in the cold.<h4>Principal findings</h4>Grown at 21 °C, mex1-1 plants were much smaller, with fewer leaves, and elevated carbohydrates and amino acids compared to WT. However, after transfer to 4 °C the total soluble sugar pool and amino acid concentration was in equal abundance in both genotypes, although the most abundant sugar in mex1-1 was still maltose whereas sucrose was in greatest abundance in WT. The chlorophyll a/b ratio in WT was much lower in the cold than in the warm, but in mex1-1 it was low in both warm and cold. After prolonged growth at 4 °C, the shoot biomass, rosette diameter and number of leaves at bolting were similar in mex1-1 and WT.<h4>Conclusions</h4>The mex1-1 mutation in warm-grown plants confers aspects of cold acclimation, including elevated levels of sugars and amino acids and low chlorophyll a/b ratio. This may in turn compromise growth of mex1-1 in the warm relative to WT. We suggest that elevated maltose in the plastid could be responsible for key aspects of cold acclimation.
format	article
author	Sarah J Purdy John D Bussell Christopher P Nunn Steven M Smith
author_facet	Sarah J Purdy John D Bussell Christopher P Nunn Steven M Smith
author_sort	Sarah J Purdy
title	Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.
title_short	Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.
title_full	Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.
title_fullStr	Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.
title_full_unstemmed	Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.
title_sort	leaves of the arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/06d54dc6236841bfac8856d26a37b10f
work_keys_str_mv	AT sarahjpurdy leavesofthearabidopsismaltoseexporter1mutantexhibitametabolicprofilewithfeaturesofcoldacclimationinthewarm AT johndbussell leavesofthearabidopsismaltoseexporter1mutantexhibitametabolicprofilewithfeaturesofcoldacclimationinthewarm AT christopherpnunn leavesofthearabidopsismaltoseexporter1mutantexhibitametabolicprofilewithfeaturesofcoldacclimationinthewarm AT stevenmsmith leavesofthearabidopsismaltoseexporter1mutantexhibitametabolicprofilewithfeaturesofcoldacclimationinthewarm
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Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm.

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