Simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes

Abstract The microgravitational environment is known to affect the cellular behaviour inducing modulation of gene expression and enzymatic activities, epigenetic modifications and alterations of the structural organization. Simulated microgravity, obtained in the laboratory setting through the use o...

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Autores principales:	Danilo Ranieri, Sara Proietti, Simona Dinicola, Maria Grazia Masiello, Benedetta Rosato, Giulia Ricci, Alessandra Cucina, Angela Catizone, Mariano Bizzarri, Maria Rosaria Torrisi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06d7f470dd1241aa9f582d46bef2e61a
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spelling	oai:doaj.org-article:06d7f470dd1241aa9f582d46bef2e61a2021-12-02T16:06:30ZSimulated microgravity triggers epithelial mesenchymal transition in human keratinocytes10.1038/s41598-017-00602-02045-2322https://doaj.org/article/06d7f470dd1241aa9f582d46bef2e61a2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00602-0https://doaj.org/toc/2045-2322Abstract The microgravitational environment is known to affect the cellular behaviour inducing modulation of gene expression and enzymatic activities, epigenetic modifications and alterations of the structural organization. Simulated microgravity, obtained in the laboratory setting through the use of a Random Positioning Machine (RPM), represents a well recognized and useful tool for the experimental studies of the cellular adaptations and molecular changes in response to weightlessness. Short exposure of cultured human keratinocytes to the RPM microgravity influences the cellular circadian clock oscillation. Therefore, here we searched for changes on the regenerative ability and response to tissue damage of human epidermal cells through the analysis of the effects of the simulated microgravity on the re-epithelialization phase of the repair and wound healing process. Combining morphological, biochemical and molecular approaches, we found that the simulated microgravity exposure of human keratinocytes promotes a migratory behavior and triggers the epithelial-mesenchymal transition (EMT) through expression of the typical EMT transcription factors and markers, such as Snail1, Snail2 and ZEB2, metalloproteases, mesenchymal adhesion molecules and cytoskeletal components.Danilo RanieriSara ProiettiSimona DinicolaMaria Grazia MasielloBenedetta RosatoGiulia RicciAlessandra CucinaAngela CatizoneMariano BizzarriMaria Rosaria TorrisiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Danilo Ranieri Sara Proietti Simona Dinicola Maria Grazia Masiello Benedetta Rosato Giulia Ricci Alessandra Cucina Angela Catizone Mariano Bizzarri Maria Rosaria Torrisi Simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes
description	Abstract The microgravitational environment is known to affect the cellular behaviour inducing modulation of gene expression and enzymatic activities, epigenetic modifications and alterations of the structural organization. Simulated microgravity, obtained in the laboratory setting through the use of a Random Positioning Machine (RPM), represents a well recognized and useful tool for the experimental studies of the cellular adaptations and molecular changes in response to weightlessness. Short exposure of cultured human keratinocytes to the RPM microgravity influences the cellular circadian clock oscillation. Therefore, here we searched for changes on the regenerative ability and response to tissue damage of human epidermal cells through the analysis of the effects of the simulated microgravity on the re-epithelialization phase of the repair and wound healing process. Combining morphological, biochemical and molecular approaches, we found that the simulated microgravity exposure of human keratinocytes promotes a migratory behavior and triggers the epithelial-mesenchymal transition (EMT) through expression of the typical EMT transcription factors and markers, such as Snail1, Snail2 and ZEB2, metalloproteases, mesenchymal adhesion molecules and cytoskeletal components.
format	article
author	Danilo Ranieri Sara Proietti Simona Dinicola Maria Grazia Masiello Benedetta Rosato Giulia Ricci Alessandra Cucina Angela Catizone Mariano Bizzarri Maria Rosaria Torrisi
author_facet	Danilo Ranieri Sara Proietti Simona Dinicola Maria Grazia Masiello Benedetta Rosato Giulia Ricci Alessandra Cucina Angela Catizone Mariano Bizzarri Maria Rosaria Torrisi
author_sort	Danilo Ranieri
title	Simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes
title_short	Simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes
title_full	Simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes
title_fullStr	Simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes
title_full_unstemmed	Simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes
title_sort	simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/06d7f470dd1241aa9f582d46bef2e61a
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Simulated microgravity triggers epithelial mesenchymal transition in human keratinocytes

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