Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis

Magnolia wufengensis (Magnoliaceae) is a deciduous landscape species, known for its ornamental value with uniquely shaped and coloured tepals. The species has been introduced to many cities in south China, but low temperatures limit the expansion of this species in cold regions. Bud dormancy is crit...

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Autores principales:	Kunjing Wu, Xiaojing Duan, Zhonglong Zhu, Ziyang Sang, Yutong Zhang, Haiying Li, Zhongkui Jia, Luyi Ma
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bud dormancy RNA-seq Magnolia wufengensis ABA dormancy release cold tolerance Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/06dae671bb2140af91d0e475018a6790
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spelling	oai:doaj.org-article:06dae671bb2140af91d0e475018a67902021-11-11T08:18:31ZTranscriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis1664-462X10.3389/fpls.2021.742504https://doaj.org/article/06dae671bb2140af91d0e475018a67902021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.742504/fullhttps://doaj.org/toc/1664-462XMagnolia wufengensis (Magnoliaceae) is a deciduous landscape species, known for its ornamental value with uniquely shaped and coloured tepals. The species has been introduced to many cities in south China, but low temperatures limit the expansion of this species in cold regions. Bud dormancy is critical for plants to survive in cold environments during the winter. In this study, we performed transcriptomic analysis of leaf buds using RNA sequencing and compared their gene expression during endodormancy, endodormancy release, and ecodormancy. A total of 187,406 unigenes were generated with an average length of 621.82 bp (N50 = 895 bp). In the transcriptomic analysis, differentially expressed genes involved in metabolism and signal transduction of hormones especially abscisic acid (ABA) were substantially annotated during dormancy transition. Our results showed that ABA at a concentration of 100 μM promoted dormancy maintenance in buds of M. wufengensis. Furthermore, the expression of genes related to ABA biosynthesis, catabolism, and signalling pathway was analysed by qPCR. We found that the expression of MwCYP707A-1-2 was consistent with ABA content and the dormancy transition phase, indicating that MwCYP707A-1-2 played a role in endodormancy release. In addition, the upregulation of MwCBF1 during dormancy release highlighted the enhancement of cold resistance. This study provides new insights into the cold tolerance of M. wufengensis in the winter from bud dormancy based on RNA-sequencing and offers fundamental data for further research on breeding improvement of M. wufengensis.Kunjing WuXiaojing DuanXiaojing DuanZhonglong ZhuZhonglong ZhuZhonglong ZhuZiyang SangYutong ZhangYutong ZhangYutong ZhangHaiying LiHaiying LiZhongkui JiaZhongkui JiaLuyi MaLuyi MaLuyi MaFrontiers Media S.A.articlebud dormancyRNA-seqMagnolia wufengensisABAdormancy releasecold tolerancePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bud dormancy RNA-seq Magnolia wufengensis ABA dormancy release cold tolerance Plant culture SB1-1110
spellingShingle	bud dormancy RNA-seq Magnolia wufengensis ABA dormancy release cold tolerance Plant culture SB1-1110 Kunjing Wu Xiaojing Duan Xiaojing Duan Zhonglong Zhu Zhonglong Zhu Zhonglong Zhu Ziyang Sang Yutong Zhang Yutong Zhang Yutong Zhang Haiying Li Haiying Li Zhongkui Jia Zhongkui Jia Luyi Ma Luyi Ma Luyi Ma Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis
description	Magnolia wufengensis (Magnoliaceae) is a deciduous landscape species, known for its ornamental value with uniquely shaped and coloured tepals. The species has been introduced to many cities in south China, but low temperatures limit the expansion of this species in cold regions. Bud dormancy is critical for plants to survive in cold environments during the winter. In this study, we performed transcriptomic analysis of leaf buds using RNA sequencing and compared their gene expression during endodormancy, endodormancy release, and ecodormancy. A total of 187,406 unigenes were generated with an average length of 621.82 bp (N50 = 895 bp). In the transcriptomic analysis, differentially expressed genes involved in metabolism and signal transduction of hormones especially abscisic acid (ABA) were substantially annotated during dormancy transition. Our results showed that ABA at a concentration of 100 μM promoted dormancy maintenance in buds of M. wufengensis. Furthermore, the expression of genes related to ABA biosynthesis, catabolism, and signalling pathway was analysed by qPCR. We found that the expression of MwCYP707A-1-2 was consistent with ABA content and the dormancy transition phase, indicating that MwCYP707A-1-2 played a role in endodormancy release. In addition, the upregulation of MwCBF1 during dormancy release highlighted the enhancement of cold resistance. This study provides new insights into the cold tolerance of M. wufengensis in the winter from bud dormancy based on RNA-sequencing and offers fundamental data for further research on breeding improvement of M. wufengensis.
format	article
author	Kunjing Wu Xiaojing Duan Xiaojing Duan Zhonglong Zhu Zhonglong Zhu Zhonglong Zhu Ziyang Sang Yutong Zhang Yutong Zhang Yutong Zhang Haiying Li Haiying Li Zhongkui Jia Zhongkui Jia Luyi Ma Luyi Ma Luyi Ma
author_facet	Kunjing Wu Xiaojing Duan Xiaojing Duan Zhonglong Zhu Zhonglong Zhu Zhonglong Zhu Ziyang Sang Yutong Zhang Yutong Zhang Yutong Zhang Haiying Li Haiying Li Zhongkui Jia Zhongkui Jia Luyi Ma Luyi Ma Luyi Ma
author_sort	Kunjing Wu
title	Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis
title_short	Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis
title_full	Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis
title_fullStr	Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis
title_full_unstemmed	Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis
title_sort	transcriptomic analysis reveals the positive role of abscisic acid in endodormancy maintenance of leaf buds of magnolia wufengensis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/06dae671bb2140af91d0e475018a6790
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Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis

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