Real-time polarization microscopy of fibrillar collagen in histopathology

Abstract Over the past two decades, fibrillar collagen reorganization parameters such as the amount of collagen deposition, fiber angle and alignment have been widely explored in numerous studies. These parameters are now widely accepted as stromal biomarkers and linked to disease progression and su...

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Autores principales:	Adib Keikhosravi, Michael Shribak, Matthew W. Conklin, Yuming Liu, Bin Li, Agnes Loeffler, Richard M. Levenson, Kevin W. Eliceiri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06e0b674fc6f4bf296a8933184a571bb
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spelling	oai:doaj.org-article:06e0b674fc6f4bf296a8933184a571bb2021-12-02T18:48:08ZReal-time polarization microscopy of fibrillar collagen in histopathology10.1038/s41598-021-98600-w2045-2322https://doaj.org/article/06e0b674fc6f4bf296a8933184a571bb2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98600-whttps://doaj.org/toc/2045-2322Abstract Over the past two decades, fibrillar collagen reorganization parameters such as the amount of collagen deposition, fiber angle and alignment have been widely explored in numerous studies. These parameters are now widely accepted as stromal biomarkers and linked to disease progression and survival time in several cancer types. Despite all these advances, there has not been a significant effort to make it possible for clinicians to explore these biomarkers without adding steps to the clinical workflow or by requiring high-cost imaging systems. In this paper, we evaluate previously described polychromatic polarization microscope (PPM) to visualize collagen fibers with an optically generated color representation of fiber orientation and alignment when inspecting the sample by a regular microscope with minor modifications. This system does not require stained slides, but is compatible with histological stains such as H&E. Consequently, it can be easily accommodated as part of regular pathology review of tissue slides, while providing clinically useful insight into stromal composition.Adib KeikhosraviMichael ShribakMatthew W. ConklinYuming LiuBin LiAgnes LoefflerRichard M. LevensonKevin W. EliceiriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adib Keikhosravi Michael Shribak Matthew W. Conklin Yuming Liu Bin Li Agnes Loeffler Richard M. Levenson Kevin W. Eliceiri Real-time polarization microscopy of fibrillar collagen in histopathology
description	Abstract Over the past two decades, fibrillar collagen reorganization parameters such as the amount of collagen deposition, fiber angle and alignment have been widely explored in numerous studies. These parameters are now widely accepted as stromal biomarkers and linked to disease progression and survival time in several cancer types. Despite all these advances, there has not been a significant effort to make it possible for clinicians to explore these biomarkers without adding steps to the clinical workflow or by requiring high-cost imaging systems. In this paper, we evaluate previously described polychromatic polarization microscope (PPM) to visualize collagen fibers with an optically generated color representation of fiber orientation and alignment when inspecting the sample by a regular microscope with minor modifications. This system does not require stained slides, but is compatible with histological stains such as H&E. Consequently, it can be easily accommodated as part of regular pathology review of tissue slides, while providing clinically useful insight into stromal composition.
format	article
author	Adib Keikhosravi Michael Shribak Matthew W. Conklin Yuming Liu Bin Li Agnes Loeffler Richard M. Levenson Kevin W. Eliceiri
author_facet	Adib Keikhosravi Michael Shribak Matthew W. Conklin Yuming Liu Bin Li Agnes Loeffler Richard M. Levenson Kevin W. Eliceiri
author_sort	Adib Keikhosravi
title	Real-time polarization microscopy of fibrillar collagen in histopathology
title_short	Real-time polarization microscopy of fibrillar collagen in histopathology
title_full	Real-time polarization microscopy of fibrillar collagen in histopathology
title_fullStr	Real-time polarization microscopy of fibrillar collagen in histopathology
title_full_unstemmed	Real-time polarization microscopy of fibrillar collagen in histopathology
title_sort	real-time polarization microscopy of fibrillar collagen in histopathology
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/06e0b674fc6f4bf296a8933184a571bb
work_keys_str_mv	AT adibkeikhosravi realtimepolarizationmicroscopyoffibrillarcollageninhistopathology AT michaelshribak realtimepolarizationmicroscopyoffibrillarcollageninhistopathology AT matthewwconklin realtimepolarizationmicroscopyoffibrillarcollageninhistopathology AT yumingliu realtimepolarizationmicroscopyoffibrillarcollageninhistopathology AT binli realtimepolarizationmicroscopyoffibrillarcollageninhistopathology AT agnesloeffler realtimepolarizationmicroscopyoffibrillarcollageninhistopathology AT richardmlevenson realtimepolarizationmicroscopyoffibrillarcollageninhistopathology AT kevinweliceiri realtimepolarizationmicroscopyoffibrillarcollageninhistopathology
_version_	1718377603384999936

Real-time polarization microscopy of fibrillar collagen in histopathology

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