Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.

The bacterial genus Klebsiella includes the closely related species K. michiganensis, K. oxytoca and K. pneumoniae, which are capable of causing severe disease in humans. In this report we describe the isolation, genomic and functional characterisation of the lytic bacteriophage KMI8 specific for K....

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Autores principales:	Heng Ku, Mwila Kabwe, Hiu Tat Chan, Cassandra Stanton, Steve Petrovski, Steven Batinovic, Joseph Tucci
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06eaedf45e0a44b3881626050aae707b
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spelling	oai:doaj.org-article:06eaedf45e0a44b3881626050aae707b2021-12-02T20:14:47ZNovel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.1932-620310.1371/journal.pone.0257102https://doaj.org/article/06eaedf45e0a44b3881626050aae707b2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257102https://doaj.org/toc/1932-6203The bacterial genus Klebsiella includes the closely related species K. michiganensis, K. oxytoca and K. pneumoniae, which are capable of causing severe disease in humans. In this report we describe the isolation, genomic and functional characterisation of the lytic bacteriophage KMI8 specific for K. michiganensis. KMI8 belongs to the family Drexlerviridae, and has a novel genome which shares very little homology (71.89% identity over a query cover of only 8%) with that of its closest related bacteriophages (Klebsiella bacteriophage LF20 (MW417503.1); Klebsiella bacteriophage 066039 (MW042802.1). KMI8, which possess a putative endosialidase (depolymerase) enzyme, was shown to be capable of degrading mono-biofilms of a strain of K. michiganensis that carried the polysaccharide capsule KL70 locus. This is the first report of a lytic bacteriophage for K. michiganensis, which is capable of breaking down a biofilm of this species.Heng KuMwila KabweHiu Tat ChanCassandra StantonSteve PetrovskiSteven BatinovicJoseph TucciPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257102 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heng Ku Mwila Kabwe Hiu Tat Chan Cassandra Stanton Steve Petrovski Steven Batinovic Joseph Tucci Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
description	The bacterial genus Klebsiella includes the closely related species K. michiganensis, K. oxytoca and K. pneumoniae, which are capable of causing severe disease in humans. In this report we describe the isolation, genomic and functional characterisation of the lytic bacteriophage KMI8 specific for K. michiganensis. KMI8 belongs to the family Drexlerviridae, and has a novel genome which shares very little homology (71.89% identity over a query cover of only 8%) with that of its closest related bacteriophages (Klebsiella bacteriophage LF20 (MW417503.1); Klebsiella bacteriophage 066039 (MW042802.1). KMI8, which possess a putative endosialidase (depolymerase) enzyme, was shown to be capable of degrading mono-biofilms of a strain of K. michiganensis that carried the polysaccharide capsule KL70 locus. This is the first report of a lytic bacteriophage for K. michiganensis, which is capable of breaking down a biofilm of this species.
format	article
author	Heng Ku Mwila Kabwe Hiu Tat Chan Cassandra Stanton Steve Petrovski Steven Batinovic Joseph Tucci
author_facet	Heng Ku Mwila Kabwe Hiu Tat Chan Cassandra Stanton Steve Petrovski Steven Batinovic Joseph Tucci
author_sort	Heng Ku
title	Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_short	Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_full	Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_fullStr	Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_full_unstemmed	Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_sort	novel drexlerviridae bacteriophage kmi8 with specific lytic activity against klebsiella michiganensis and its biofilms.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/06eaedf45e0a44b3881626050aae707b
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Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.

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