Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.

The bacterial genus Klebsiella includes the closely related species K. michiganensis, K. oxytoca and K. pneumoniae, which are capable of causing severe disease in humans. In this report we describe the isolation, genomic and functional characterisation of the lytic bacteriophage KMI8 specific for K....

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Autores principales: Heng Ku, Mwila Kabwe, Hiu Tat Chan, Cassandra Stanton, Steve Petrovski, Steven Batinovic, Joseph Tucci
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Publicado: Public Library of Science (PLoS) 2021
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Acceso en línea:https://doaj.org/article/06eaedf45e0a44b3881626050aae707b
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spelling oai:doaj.org-article:06eaedf45e0a44b3881626050aae707b2021-12-02T20:14:47ZNovel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.1932-620310.1371/journal.pone.0257102https://doaj.org/article/06eaedf45e0a44b3881626050aae707b2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257102https://doaj.org/toc/1932-6203The bacterial genus Klebsiella includes the closely related species K. michiganensis, K. oxytoca and K. pneumoniae, which are capable of causing severe disease in humans. In this report we describe the isolation, genomic and functional characterisation of the lytic bacteriophage KMI8 specific for K. michiganensis. KMI8 belongs to the family Drexlerviridae, and has a novel genome which shares very little homology (71.89% identity over a query cover of only 8%) with that of its closest related bacteriophages (Klebsiella bacteriophage LF20 (MW417503.1); Klebsiella bacteriophage 066039 (MW042802.1). KMI8, which possess a putative endosialidase (depolymerase) enzyme, was shown to be capable of degrading mono-biofilms of a strain of K. michiganensis that carried the polysaccharide capsule KL70 locus. This is the first report of a lytic bacteriophage for K. michiganensis, which is capable of breaking down a biofilm of this species.Heng KuMwila KabweHiu Tat ChanCassandra StantonSteve PetrovskiSteven BatinovicJoseph TucciPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257102 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Heng Ku
Mwila Kabwe
Hiu Tat Chan
Cassandra Stanton
Steve Petrovski
Steven Batinovic
Joseph Tucci
Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
description The bacterial genus Klebsiella includes the closely related species K. michiganensis, K. oxytoca and K. pneumoniae, which are capable of causing severe disease in humans. In this report we describe the isolation, genomic and functional characterisation of the lytic bacteriophage KMI8 specific for K. michiganensis. KMI8 belongs to the family Drexlerviridae, and has a novel genome which shares very little homology (71.89% identity over a query cover of only 8%) with that of its closest related bacteriophages (Klebsiella bacteriophage LF20 (MW417503.1); Klebsiella bacteriophage 066039 (MW042802.1). KMI8, which possess a putative endosialidase (depolymerase) enzyme, was shown to be capable of degrading mono-biofilms of a strain of K. michiganensis that carried the polysaccharide capsule KL70 locus. This is the first report of a lytic bacteriophage for K. michiganensis, which is capable of breaking down a biofilm of this species.
format article
author Heng Ku
Mwila Kabwe
Hiu Tat Chan
Cassandra Stanton
Steve Petrovski
Steven Batinovic
Joseph Tucci
author_facet Heng Ku
Mwila Kabwe
Hiu Tat Chan
Cassandra Stanton
Steve Petrovski
Steven Batinovic
Joseph Tucci
author_sort Heng Ku
title Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_short Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_full Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_fullStr Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_full_unstemmed Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms.
title_sort novel drexlerviridae bacteriophage kmi8 with specific lytic activity against klebsiella michiganensis and its biofilms.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/06eaedf45e0a44b3881626050aae707b
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