Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection

Abstract Metabonomics/metabolomics is a rapid technology for comprehensive profiling of small molecule metabolites in cells, tissues, or whole organisms, the application of which has led to understanding pathophysiologic mechanisms of cardiometabolic diseases, defining predictive biomarkers for thos...

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Autores principales:	Miaomiao Jiang, Qiuying Wang, Jingrui Chen, Yanan Wang, Guanwei Fan, Yan Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06efd62254a64f249d0b71074ae99d4b
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spelling	oai:doaj.org-article:06efd62254a64f249d0b71074ae99d4b2021-12-02T15:05:46ZComparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection10.1038/s41598-017-09547-w2045-2322https://doaj.org/article/06efd62254a64f249d0b71074ae99d4b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09547-whttps://doaj.org/toc/2045-2322Abstract Metabonomics/metabolomics is a rapid technology for comprehensive profiling of small molecule metabolites in cells, tissues, or whole organisms, the application of which has led to understanding pathophysiologic mechanisms of cardiometabolic diseases, defining predictive biomarkers for those diseases, and also assessing the efficacious effects of incident drugs. In this study, proton nuclear magnetic resonance (NMR)-based metabonomics was employed to identify the metabolic changes in rat plasma caused by myocardial ischemia-reperfusion injury (MIRI), and to compare the metabolic regulatory differences between traditional Chinese medicine Wenxin Keli (WXKL) and Western medicine verapamil. The results revealed that energy-substrate metabolism were significantly disturbed by ischemia-reperfusion (I/R) in myocardium and bulk of the key metabolites could be further modulated by verapamil and/or WXKL. Lipid metabolism and amino acid transamination occurred mainly following the treatment of verapamil, whereas glucose oxidation and BCAA degradation were prominently ameliorated by WXKL to content the energy demands of heart. Moreover, both WXKL and verapamil improved the secretions of taurine and ketone bodies to overcome the oxidative stress and the shortage of energy sources induced by ischemia-reperfusion.Miaomiao JiangQiuying WangJingrui ChenYanan WangGuanwei FanYan ZhuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miaomiao Jiang Qiuying Wang Jingrui Chen Yanan Wang Guanwei Fan Yan Zhu Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection
description	Abstract Metabonomics/metabolomics is a rapid technology for comprehensive profiling of small molecule metabolites in cells, tissues, or whole organisms, the application of which has led to understanding pathophysiologic mechanisms of cardiometabolic diseases, defining predictive biomarkers for those diseases, and also assessing the efficacious effects of incident drugs. In this study, proton nuclear magnetic resonance (NMR)-based metabonomics was employed to identify the metabolic changes in rat plasma caused by myocardial ischemia-reperfusion injury (MIRI), and to compare the metabolic regulatory differences between traditional Chinese medicine Wenxin Keli (WXKL) and Western medicine verapamil. The results revealed that energy-substrate metabolism were significantly disturbed by ischemia-reperfusion (I/R) in myocardium and bulk of the key metabolites could be further modulated by verapamil and/or WXKL. Lipid metabolism and amino acid transamination occurred mainly following the treatment of verapamil, whereas glucose oxidation and BCAA degradation were prominently ameliorated by WXKL to content the energy demands of heart. Moreover, both WXKL and verapamil improved the secretions of taurine and ketone bodies to overcome the oxidative stress and the shortage of energy sources induced by ischemia-reperfusion.
format	article
author	Miaomiao Jiang Qiuying Wang Jingrui Chen Yanan Wang Guanwei Fan Yan Zhu
author_facet	Miaomiao Jiang Qiuying Wang Jingrui Chen Yanan Wang Guanwei Fan Yan Zhu
author_sort	Miaomiao Jiang
title	Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection
title_short	Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection
title_full	Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection
title_fullStr	Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection
title_full_unstemmed	Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection
title_sort	comparative metabonomics of wenxin keli and verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/06efd62254a64f249d0b71074ae99d4b
work_keys_str_mv	AT miaomiaojiang comparativemetabonomicsofwenxinkeliandverapamilrevealsdifferentialrolesofgluconeogenesisandfattyacidboxidationinmyocardialinjuryprotection AT qiuyingwang comparativemetabonomicsofwenxinkeliandverapamilrevealsdifferentialrolesofgluconeogenesisandfattyacidboxidationinmyocardialinjuryprotection AT jingruichen comparativemetabonomicsofwenxinkeliandverapamilrevealsdifferentialrolesofgluconeogenesisandfattyacidboxidationinmyocardialinjuryprotection AT yananwang comparativemetabonomicsofwenxinkeliandverapamilrevealsdifferentialrolesofgluconeogenesisandfattyacidboxidationinmyocardialinjuryprotection AT guanweifan comparativemetabonomicsofwenxinkeliandverapamilrevealsdifferentialrolesofgluconeogenesisandfattyacidboxidationinmyocardialinjuryprotection AT yanzhu comparativemetabonomicsofwenxinkeliandverapamilrevealsdifferentialrolesofgluconeogenesisandfattyacidboxidationinmyocardialinjuryprotection
_version_	1718388685862338560

Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection

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