In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis.

<h4>Purpose</h4>To assess the in vitro larvicidal activity of ivermectin and povidone-iodine (PVP-I) against Oestrus ovis, the most frequent cause of external ophthalmomyiasis.<h4>Methods</h4>L1 O. ovis larvae were collected from the nasal boots of sheep slaughtered in local...

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Autores principales: Giuseppe D'Amico Ricci, Giovanni Garippa, Stefano Cortese, Rita Serra, Francesco Boscia, Stefano Dore, Antonio Pinna
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Publicado: Public Library of Science (PLoS) 2021
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spelling oai:doaj.org-article:06f020f20767418ca43f3e2d661a6f762021-12-02T20:13:28ZIn vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis.1932-620310.1371/journal.pone.0259044https://doaj.org/article/06f020f20767418ca43f3e2d661a6f762021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259044https://doaj.org/toc/1932-6203<h4>Purpose</h4>To assess the in vitro larvicidal activity of ivermectin and povidone-iodine (PVP-I) against Oestrus ovis, the most frequent cause of external ophthalmomyiasis.<h4>Methods</h4>L1 O. ovis larvae were collected from the nasal boots of sheep slaughtered in local abattoirs and transferred onto Petri dishes containing mucosal tissue (25 larvae/dish). The larvicidal activity of the following formulations was tested: 1% ivermectin suspension in balanced sterile saline solution (BSSS), 1% ivermectin solution in propylene glycol, propylene glycol, 0.6% PVP-I in hyaluronic acid vehicle (IODIM®), and combination of ivermectin 1% solution and 0.6% PVP-I. One mL of each formulation was added to different Petri dishes containing the larvae. The time needed to kill the larvae was recorded.<h4>Results</h4>893 larvae were tested. The median time needed to kill the larvae was 46, 44, 11, 6, and 10 minutes for Iodim®, ivermectin 1% suspension, propylene glycol, ivermectin 1% solution, and a combination of ivermectin 1% solution with 0.6% PVP-I, respectively. Kaplan-Meyer analysis disclosed that the survival curves were significantly lower in samples treated with ivermectin 1% solution, ivermectin 1% solution + 0.6% PVP-I, and propylene glycol than in samples receiving other treatments or BSSS.<h4>Conclusion</h4>In this in vitro study, ivermectin 1% solution in propylene glycol, ivermectin 1% solution + 0.6% PVP-I, and propylene glycol alone showed a good, relatively rapid larvicidal activity against O. ovis larvae. Further experimental and clinical studies are necessary to establish whether, or not, these formulations may be considered as potential candidates for the topical treatment for external ophthalmomyiasis caused by O. ovis.Giuseppe D'Amico RicciGiovanni GarippaStefano CorteseRita SerraFrancesco BosciaStefano DoreAntonio PinnaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0259044 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Giuseppe D'Amico Ricci
Giovanni Garippa
Stefano Cortese
Rita Serra
Francesco Boscia
Stefano Dore
Antonio Pinna
In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis.
description <h4>Purpose</h4>To assess the in vitro larvicidal activity of ivermectin and povidone-iodine (PVP-I) against Oestrus ovis, the most frequent cause of external ophthalmomyiasis.<h4>Methods</h4>L1 O. ovis larvae were collected from the nasal boots of sheep slaughtered in local abattoirs and transferred onto Petri dishes containing mucosal tissue (25 larvae/dish). The larvicidal activity of the following formulations was tested: 1% ivermectin suspension in balanced sterile saline solution (BSSS), 1% ivermectin solution in propylene glycol, propylene glycol, 0.6% PVP-I in hyaluronic acid vehicle (IODIM®), and combination of ivermectin 1% solution and 0.6% PVP-I. One mL of each formulation was added to different Petri dishes containing the larvae. The time needed to kill the larvae was recorded.<h4>Results</h4>893 larvae were tested. The median time needed to kill the larvae was 46, 44, 11, 6, and 10 minutes for Iodim®, ivermectin 1% suspension, propylene glycol, ivermectin 1% solution, and a combination of ivermectin 1% solution with 0.6% PVP-I, respectively. Kaplan-Meyer analysis disclosed that the survival curves were significantly lower in samples treated with ivermectin 1% solution, ivermectin 1% solution + 0.6% PVP-I, and propylene glycol than in samples receiving other treatments or BSSS.<h4>Conclusion</h4>In this in vitro study, ivermectin 1% solution in propylene glycol, ivermectin 1% solution + 0.6% PVP-I, and propylene glycol alone showed a good, relatively rapid larvicidal activity against O. ovis larvae. Further experimental and clinical studies are necessary to establish whether, or not, these formulations may be considered as potential candidates for the topical treatment for external ophthalmomyiasis caused by O. ovis.
format article
author Giuseppe D'Amico Ricci
Giovanni Garippa
Stefano Cortese
Rita Serra
Francesco Boscia
Stefano Dore
Antonio Pinna
author_facet Giuseppe D'Amico Ricci
Giovanni Garippa
Stefano Cortese
Rita Serra
Francesco Boscia
Stefano Dore
Antonio Pinna
author_sort Giuseppe D'Amico Ricci
title In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis.
title_short In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis.
title_full In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis.
title_fullStr In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis.
title_full_unstemmed In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis.
title_sort in vitro larvicidal activity of ivermectin and povidone-iodine against oestrus ovis.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/06f020f20767418ca43f3e2d661a6f76
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