miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus

Gestational diabetes mellitus (GDM) is a metabolic dysregulation closely related to both obesity and type 2 diabetes; however, the molecular mechanism underlying GDM is still unclear. The purpose of this study was to investigate the effects of microRNA-377 (miR-377-3p) and fibronectin type III domai...

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Autores principales:	Hua Zhaozhao, Li Dana, Wu Anqin, Cao Ting, Luo Shi
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	gestational diabetes mellitus mir-377-3p fndc5 cell survival trophoblast cells Medicine R
Acceso en línea:	https://doaj.org/article/06f1a048477b4a2eb482ee2028066d22
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id	oai:doaj.org-article:06f1a048477b4a2eb482ee2028066d22
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spelling	oai:doaj.org-article:06f1a048477b4a2eb482ee2028066d222021-12-05T14:10:54ZmiR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus2391-546310.1515/med-2021-0247https://doaj.org/article/06f1a048477b4a2eb482ee2028066d222021-03-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0247https://doaj.org/toc/2391-5463Gestational diabetes mellitus (GDM) is a metabolic dysregulation closely related to both obesity and type 2 diabetes; however, the molecular mechanism underlying GDM is still unclear. The purpose of this study was to investigate the effects of microRNA-377 (miR-377-3p) and fibronectin type III domain containing 5 (FNDC5) in regulating the cell growth of trophoblasts under high glucose (HG) conditions during the development of GDM. Serum miR-377-3p was upregulated and positively correlated with fasting blood glucose level in GDM patients. miR-377-3p downregulation increased the cell vitality and suppressed the cell apoptosis of HG-treated HTR-8/SVneo and BeWo cells. Using TargetScan prediction, luciferase assay, and western blot, it was found that miR-377-3p could target FNDC5 and suppress its expression. However, FNDC5 downregulation abolished the effect of miR-377-3p inhibitor in HTR-8/SVneo cells. Together, miR-377 is a potential target for GDM biomarker, which promotes cell growth and suppresses cell apoptosis, partly through the upregulation of FNDC5.Hua ZhaozhaoLi DanaWu AnqinCao TingLuo ShiDe Gruyterarticlegestational diabetes mellitusmir-377-3pfndc5cell survivaltrophoblast cellsMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 464-471 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gestational diabetes mellitus mir-377-3p fndc5 cell survival trophoblast cells Medicine R
spellingShingle	gestational diabetes mellitus mir-377-3p fndc5 cell survival trophoblast cells Medicine R Hua Zhaozhao Li Dana Wu Anqin Cao Ting Luo Shi miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus
description	Gestational diabetes mellitus (GDM) is a metabolic dysregulation closely related to both obesity and type 2 diabetes; however, the molecular mechanism underlying GDM is still unclear. The purpose of this study was to investigate the effects of microRNA-377 (miR-377-3p) and fibronectin type III domain containing 5 (FNDC5) in regulating the cell growth of trophoblasts under high glucose (HG) conditions during the development of GDM. Serum miR-377-3p was upregulated and positively correlated with fasting blood glucose level in GDM patients. miR-377-3p downregulation increased the cell vitality and suppressed the cell apoptosis of HG-treated HTR-8/SVneo and BeWo cells. Using TargetScan prediction, luciferase assay, and western blot, it was found that miR-377-3p could target FNDC5 and suppress its expression. However, FNDC5 downregulation abolished the effect of miR-377-3p inhibitor in HTR-8/SVneo cells. Together, miR-377 is a potential target for GDM biomarker, which promotes cell growth and suppresses cell apoptosis, partly through the upregulation of FNDC5.
format	article
author	Hua Zhaozhao Li Dana Wu Anqin Cao Ting Luo Shi
author_facet	Hua Zhaozhao Li Dana Wu Anqin Cao Ting Luo Shi
author_sort	Hua Zhaozhao
title	miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus
title_short	miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus
title_full	miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus
title_fullStr	miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus
title_full_unstemmed	miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus
title_sort	mir-377 inhibition enhances the survival of trophoblast cells via upregulation of fndc5 in gestational diabetes mellitus
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/06f1a048477b4a2eb482ee2028066d22
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miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus

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