Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method

Genome mining study showed that marine-derived Streptomyces sp. GMY01 is a potential actinobacteria with abundant of secondary metabolite and anticancer activity. The study aimed to evaluate bioassay guided fractionation for antiplasmodial screening of GMY01 extract and to predict compound on active...

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Autores principales:	Ema Damayanti, Jaka Widada, Puspa Dewi N. Lotulung, Achmad Dinoto, Mustofa Mustofa
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2020
Materias:	drug discovery malaria antiplasmodial assay actinomycetes Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/06f54a8cb4134c538460cc8007f577fa
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spelling	oai:doaj.org-article:06f54a8cb4134c538460cc8007f577fa2021-11-15T06:08:47ZBioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method2338-94272338-948610.22146/ijp.809https://doaj.org/article/06f54a8cb4134c538460cc8007f577fa2020-12-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/809https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Genome mining study showed that marine-derived Streptomyces sp. GMY01 is a potential actinobacteria with abundant of secondary metabolite and anticancer activity. The study aimed to evaluate bioassay guided fractionation for antiplasmodial screening of GMY01 extract and to predict compound on active fractions. Ethyl acetate fraction was obtained from 11 days fermentation product of GMY01 and then it was fractionated using n-hexane and methanol solvent. Refractionated was applied using flash chromatography and column chromatography. Antiplasmodial assay was performed on Plasmodium falciparum FCR3 by microscopic method using thin blood smear + Giemsa stain, and flow cytometry method using SYBR Green I stain. Toxicity assay was performed on Vero cells line. Main constituent of active fraction was analyzed using LCMS/MS. The result of the study showed that ethyl acetate-methanol fraction has high antiplasmodial activity (IC50=3.96 µg/mL) with very low toxicity on Vero cells (IC50=30,072 µg/mL). Bioassay guided fractionation resulted F4.7 as highest Plasmodium inhibition (94.3% at 5 µg/mL) and was confirmed by microscopic and flow cytometry assay. Main constituent analysis showed C10H13NO (163.09971 Da) as mayor compound and predicted as nonribosomal polyketide synthetase (NRPS) secondary metabolite.Ema DamayantiJaka WidadaPuspa Dewi N. LotulungAchmad DinotoMustofa MustofaUniversitas Gadjah Madaarticledrug discoverymalariaantiplasmodial assayactinomycetesPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 281–289-281–289 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	drug discovery malaria antiplasmodial assay actinomycetes Pharmacy and materia medica RS1-441
spellingShingle	drug discovery malaria antiplasmodial assay actinomycetes Pharmacy and materia medica RS1-441 Ema Damayanti Jaka Widada Puspa Dewi N. Lotulung Achmad Dinoto Mustofa Mustofa Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method
description	Genome mining study showed that marine-derived Streptomyces sp. GMY01 is a potential actinobacteria with abundant of secondary metabolite and anticancer activity. The study aimed to evaluate bioassay guided fractionation for antiplasmodial screening of GMY01 extract and to predict compound on active fractions. Ethyl acetate fraction was obtained from 11 days fermentation product of GMY01 and then it was fractionated using n-hexane and methanol solvent. Refractionated was applied using flash chromatography and column chromatography. Antiplasmodial assay was performed on Plasmodium falciparum FCR3 by microscopic method using thin blood smear + Giemsa stain, and flow cytometry method using SYBR Green I stain. Toxicity assay was performed on Vero cells line. Main constituent of active fraction was analyzed using LCMS/MS. The result of the study showed that ethyl acetate-methanol fraction has high antiplasmodial activity (IC50=3.96 µg/mL) with very low toxicity on Vero cells (IC50=30,072 µg/mL). Bioassay guided fractionation resulted F4.7 as highest Plasmodium inhibition (94.3% at 5 µg/mL) and was confirmed by microscopic and flow cytometry assay. Main constituent analysis showed C10H13NO (163.09971 Da) as mayor compound and predicted as nonribosomal polyketide synthetase (NRPS) secondary metabolite.
format	article
author	Ema Damayanti Jaka Widada Puspa Dewi N. Lotulung Achmad Dinoto Mustofa Mustofa
author_facet	Ema Damayanti Jaka Widada Puspa Dewi N. Lotulung Achmad Dinoto Mustofa Mustofa
author_sort	Ema Damayanti
title	Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method
title_short	Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method
title_full	Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method
title_fullStr	Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method
title_full_unstemmed	Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method
title_sort	bioassay guided fractionation of marine streptomyces sp. gmy01 and antiplasmodial assay using microscopic and flow cytometry method
publisher	Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/06f54a8cb4134c538460cc8007f577fa
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_version_	1718428534762897408

Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method

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