Characteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A Comparative Study
Xin Kang,1,2 Libin Zhan,1,3 Xiaoguang Lu,2 Jianbo Song,2 Yilong Zhong,2 Yi Wang,1 Yilun Yang,4 Zhiwei Fan,2 Xiaozheng Jiang,2 Ruru Sun2 1Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, People’s Republic of China; 2Department of Emergency Medicine, Affiliated...
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Dove Medical Press
2020
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gastric microbiota type-2 diabetes mellitus gk rat wistar rat 16s rrna gene sequencing Specialties of internal medicine RC581-951 |
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gastric microbiota type-2 diabetes mellitus gk rat wistar rat 16s rrna gene sequencing Specialties of internal medicine RC581-951 Kang X Zhan L Lu X Song J Zhong Y Wang Y Yang Y Fan Z Jiang X Sun R Characteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A Comparative Study |
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Xin Kang,1,2 Libin Zhan,1,3 Xiaoguang Lu,2 Jianbo Song,2 Yilong Zhong,2 Yi Wang,1 Yilun Yang,4 Zhiwei Fan,2 Xiaozheng Jiang,2 Ruru Sun2 1Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, People’s Republic of China; 2Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian University, Dalian, Liaoning, People’s Republic of China; 3Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 4Graduate School, Zunyi Medical University, Zunyi, Guizhou, Republic of ChinaCorrespondence: Libin ZhanInstitute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, People’s Republic of China, Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of ChinaTel +86 25-85811569Email zlbnj@njucm.edu.cnXiaoguang LuDepartment of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian University, Dalian, Liaoning, People’s Republic of ChinaEmail luxiaoguang@dlu.edu.cnBackground: The Goto-Kakizaki (GK) rat, developed from repeated inbreeding of glucose-intolerant Wistar rats, has been widely used to explore the development of spontaneous type-2 diabetes mellitus (T2DM). However, the gastric microbiota of GK and Wistar rats are still unclear. This study aimed to understand the gastric microbiota characteristics of GK rats by comparing it with non-diabetic Wistar rats.Materials and Methods: Male Wistar rats and GK rats were housed in specific pathogen-free (SPF) environment for 12 weeks with free access to sterilized food and water. Body weight and random blood glucose (BG) levels were determined. At the end of the experiment, the gastric contents of the rats were collected for the identification of gastric microbiota using 16S rRNA gene sequencing.Results: The richness of gastric microbiota in GK rats was similar to that of Wistar rats (P > 0.05). The results of Shannon, Simpson, beta diversity indices, and ANOSIM analysis showed that alpha and beta diversity of gastric microbiota in GK rats were significantly lower than that of Wistar rats (P < 0.01). Firmicutes (96.0%), Proteobacteria (1.9%) and Cyanobacteria (0.8%) were the dominant gastric microbiota in GK rats accounting for 72.9%, 14.7% and 10.9%, respectively. Linear discriminant analysis effect size (LEfSe)  revealed  that phylum Firmicutes and four genera (Anaerovibrio, Collinsella, Prevotellaceae_UCG_001, and Lactobacillus) were significantly abundant in the stomachs of GK rats. In contrast, seven genera (unidentified_Chloroplast, Porphyromonas, Neisseria, Rubrobacter, Veillonella, Lachnospiraceae_UCG_005, and unidentified_Erysipelotrichaceae) were significantly abundant in the stomachs of Wistar rats. Blood glucose was positively correlated with Anaerobibrio and Lactobacillus, and negatively correlated with four genera (Porphyromonas, Rubrobacter, Lachnospiraceae_UCG_005, and unidentified_Erysipelotrichaceae). In addition, chemoheterotrophy and fermentation were the most important functions of gastric microbiota.Conclusion: The gastric microbiota of GK rats with spontaneous T2DM showed the typical characteristics of low diversity and significant enrichment of Firmicutes phylum and four genera (Anaerovibrio, Collinsella, Prevotellaceae_UCG_001, and Lactobacillus) compared with gastric microbiota of Wistar rats.Keywords: gastric microbiota, type-2 diabetes mellitus, GK rat, wistar rat, 16S rRNA gene sequencing |
| format |
article |
| author |
Kang X Zhan L Lu X Song J Zhong Y Wang Y Yang Y Fan Z Jiang X Sun R |
| author_facet |
Kang X Zhan L Lu X Song J Zhong Y Wang Y Yang Y Fan Z Jiang X Sun R |
| author_sort |
Kang X |
| title |
Characteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A Comparative Study |
| title_short |
Characteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A Comparative Study |
| title_full |
Characteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A Comparative Study |
| title_fullStr |
Characteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A Comparative Study |
| title_full_unstemmed |
Characteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A Comparative Study |
| title_sort |
characteristics of gastric microbiota in gk rats with spontaneous diabetes: a comparative study |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/06f86e38d49243058f02b8515fc800a4 |
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1718387408936894464 |
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oai:doaj.org-article:06f86e38d49243058f02b8515fc800a42021-12-02T15:20:47ZCharacteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A Comparative Study1178-7007https://doaj.org/article/06f86e38d49243058f02b8515fc800a42020-04-01T00:00:00Zhttps://www.dovepress.com/characteristics-of-gastric-microbiota-in-gk-rats-with-spontaneous-diab-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Xin Kang,1,2 Libin Zhan,1,3 Xiaoguang Lu,2 Jianbo Song,2 Yilong Zhong,2 Yi Wang,1 Yilun Yang,4 Zhiwei Fan,2 Xiaozheng Jiang,2 Ruru Sun2 1Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, People’s Republic of China; 2Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian University, Dalian, Liaoning, People’s Republic of China; 3Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 4Graduate School, Zunyi Medical University, Zunyi, Guizhou, Republic of ChinaCorrespondence: Libin ZhanInstitute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, People’s Republic of China, Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of ChinaTel +86 25-85811569Email zlbnj@njucm.edu.cnXiaoguang LuDepartment of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian University, Dalian, Liaoning, People’s Republic of ChinaEmail luxiaoguang@dlu.edu.cnBackground: The Goto-Kakizaki (GK) rat, developed from repeated inbreeding of glucose-intolerant Wistar rats, has been widely used to explore the development of spontaneous type-2 diabetes mellitus (T2DM). However, the gastric microbiota of GK and Wistar rats are still unclear. This study aimed to understand the gastric microbiota characteristics of GK rats by comparing it with non-diabetic Wistar rats.Materials and Methods: Male Wistar rats and GK rats were housed in specific pathogen-free (SPF) environment for 12 weeks with free access to sterilized food and water. Body weight and random blood glucose (BG) levels were determined. At the end of the experiment, the gastric contents of the rats were collected for the identification of gastric microbiota using 16S rRNA gene sequencing.Results: The richness of gastric microbiota in GK rats was similar to that of Wistar rats (P > 0.05). The results of Shannon, Simpson, beta diversity indices, and ANOSIM analysis showed that alpha and beta diversity of gastric microbiota in GK rats were significantly lower than that of Wistar rats (P < 0.01). Firmicutes (96.0%), Proteobacteria (1.9%) and Cyanobacteria (0.8%) were the dominant gastric microbiota in GK rats accounting for 72.9%, 14.7% and 10.9%, respectively. Linear discriminant analysis effect size (LEfSe)  revealed  that phylum Firmicutes and four genera (Anaerovibrio, Collinsella, Prevotellaceae_UCG_001, and Lactobacillus) were significantly abundant in the stomachs of GK rats. In contrast, seven genera (unidentified_Chloroplast, Porphyromonas, Neisseria, Rubrobacter, Veillonella, Lachnospiraceae_UCG_005, and unidentified_Erysipelotrichaceae) were significantly abundant in the stomachs of Wistar rats. Blood glucose was positively correlated with Anaerobibrio and Lactobacillus, and negatively correlated with four genera (Porphyromonas, Rubrobacter, Lachnospiraceae_UCG_005, and unidentified_Erysipelotrichaceae). In addition, chemoheterotrophy and fermentation were the most important functions of gastric microbiota.Conclusion: The gastric microbiota of GK rats with spontaneous T2DM showed the typical characteristics of low diversity and significant enrichment of Firmicutes phylum and four genera (Anaerovibrio, Collinsella, Prevotellaceae_UCG_001, and Lactobacillus) compared with gastric microbiota of Wistar rats.Keywords: gastric microbiota, type-2 diabetes mellitus, GK rat, wistar rat, 16S rRNA gene sequencingKang XZhan LLu XSong JZhong YWang YYang YFan ZJiang XSun RDove Medical Pressarticlegastric microbiotatype-2 diabetes mellitusgk ratwistar rat16s rrna gene sequencingSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 1435-1447 (2020) |