Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)

Aspergillus flavus is an opportunistic fungal pathogen that produces carcinogenic aflatoxin, a serious constraint for food safety and human health. In this study, to better understand the molecular mechanism/s of peanut resistance to A. flavus growth and aflatoxin accumulation, comparative proteomic...

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Autores principales:	Pooja Bhatnagar-Mathur, Kalenahalli Yogendra, Santisree Parankusam, Hemlatha Sanivarapu, Kalyani Prasad, Shiva Bhargavi Lingampali, Kiran Kumar Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	aflatoxin arachis hypogaea aspergillus flavus flavonoids peanut proteomics phenylpropanoids Plant culture SB1-1110 Plant ecology QK900-989
Acceso en línea:	https://doaj.org/article/06fbb99cbffc4291a41750c02519becf
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spelling	oai:doaj.org-article:06fbb99cbffc4291a41750c02519becf2021-11-17T14:21:57ZComparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)1742-91451742-915310.1080/17429145.2021.1995058https://doaj.org/article/06fbb99cbffc4291a41750c02519becf2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/17429145.2021.1995058https://doaj.org/toc/1742-9145https://doaj.org/toc/1742-9153Aspergillus flavus is an opportunistic fungal pathogen that produces carcinogenic aflatoxin, a serious constraint for food safety and human health. In this study, to better understand the molecular mechanism/s of peanut resistance to A. flavus growth and aflatoxin accumulation, comparative proteomic analysis was performed in two contrasting peanut genotypes, variety JL 24 (susceptible) and its near-isogenic resistant transgenic derivative expressing an alfalfa defensin gene. Several resistance proteins associated with secondary metabolic pathways were strongly induced in the resistant genotypes including phenylalanine ammonia lyase, cinnamic acid-4-hydroxylase, chalcone synthase, resveratrol synthase, flavanone-3-hydroxylase, lipoxygenase, diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase, β-ketoacyl-ACP-reductase, monoacylglycerol acyltransferase, and diacylglycerol acyltransferase, indicating their roles in resistance. Besides, several putative susceptibility-associated proteins were revealed providing knowledge on potential candidate target genes for precise breeding interventions for aflatoxin mitigation. This is the first study to demonstrate comparative proteomics analysis in Aspergillus–peanut interaction using contrasting near-isogenic lines to elucidate the underlying molecular mechanisms of resistance.Pooja Bhatnagar-MathurKalenahalli YogendraSantisree ParankusamHemlatha SanivarapuKalyani PrasadShiva Bhargavi LingampaliKiran Kumar SharmaTaylor & Francis Grouparticleaflatoxinarachis hypogaeaaspergillus flavusflavonoidspeanutproteomicsphenylpropanoidsPlant cultureSB1-1110Plant ecologyQK900-989ENJournal of Plant Interactions, Vol 16, Iss 1, Pp 494-509 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aflatoxin arachis hypogaea aspergillus flavus flavonoids peanut proteomics phenylpropanoids Plant culture SB1-1110 Plant ecology QK900-989
spellingShingle	aflatoxin arachis hypogaea aspergillus flavus flavonoids peanut proteomics phenylpropanoids Plant culture SB1-1110 Plant ecology QK900-989 Pooja Bhatnagar-Mathur Kalenahalli Yogendra Santisree Parankusam Hemlatha Sanivarapu Kalyani Prasad Shiva Bhargavi Lingampali Kiran Kumar Sharma Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)
description	Aspergillus flavus is an opportunistic fungal pathogen that produces carcinogenic aflatoxin, a serious constraint for food safety and human health. In this study, to better understand the molecular mechanism/s of peanut resistance to A. flavus growth and aflatoxin accumulation, comparative proteomic analysis was performed in two contrasting peanut genotypes, variety JL 24 (susceptible) and its near-isogenic resistant transgenic derivative expressing an alfalfa defensin gene. Several resistance proteins associated with secondary metabolic pathways were strongly induced in the resistant genotypes including phenylalanine ammonia lyase, cinnamic acid-4-hydroxylase, chalcone synthase, resveratrol synthase, flavanone-3-hydroxylase, lipoxygenase, diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase, β-ketoacyl-ACP-reductase, monoacylglycerol acyltransferase, and diacylglycerol acyltransferase, indicating their roles in resistance. Besides, several putative susceptibility-associated proteins were revealed providing knowledge on potential candidate target genes for precise breeding interventions for aflatoxin mitigation. This is the first study to demonstrate comparative proteomics analysis in Aspergillus–peanut interaction using contrasting near-isogenic lines to elucidate the underlying molecular mechanisms of resistance.
format	article
author	Pooja Bhatnagar-Mathur Kalenahalli Yogendra Santisree Parankusam Hemlatha Sanivarapu Kalyani Prasad Shiva Bhargavi Lingampali Kiran Kumar Sharma
author_facet	Pooja Bhatnagar-Mathur Kalenahalli Yogendra Santisree Parankusam Hemlatha Sanivarapu Kalyani Prasad Shiva Bhargavi Lingampali Kiran Kumar Sharma
author_sort	Pooja Bhatnagar-Mathur
title	Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)
title_short	Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)
title_full	Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)
title_fullStr	Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)
title_full_unstemmed	Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)
title_sort	comparative proteomics provide insights on the basis of resistance to aspergillus flavus infection and aflatoxin production in peanut (arachis hypogea l.)
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/06fbb99cbffc4291a41750c02519becf
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Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)

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