Gradient Internal Standard Method for Absolute Quantification of Microbial Amplicon Sequencing Data

ABSTRACT High-throughput amplicon sequencing is a critical tool for studying microbiota; however, it results only in relative abundance data. Thus, changes in absolute abundance of microbiota cannot be determined, which hinders further microbiology research. We have therefore established a gradient...

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Bibliographic Details
Main Authors: Shilei Wang, Qun Wu, Ying Han, Rubing Du, Xiaoyong Wang, Yao Nie, Xiaowei Du, Yan Xu
Format: article
Language:EN
Published: American Society for Microbiology 2021
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Online Access:https://doaj.org/article/06fcc614b6ba473390b51fe9e0d65773
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