Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals

Abstract Neural recordings made to date through various approaches—both in-vitro or in-vivo—lack high spatial resolution and a high signal-to-noise ratio (SNR) required for detailed understanding of brain function, synaptic plasticity, and dysfunction. These shortcomings in turn deter the ability to...

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Autores principales:	P. Wijdenes, K. Haider, C. Gavrilovici, B. Gunning, M. D. Wolff, T. Lijnse, R. Armstrong, G. C. Teskey, J. M. Rho, C. Dalton, Naweed I. Syed
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07111a50d0a94ec8bdbd64b85d857050
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spelling	oai:doaj.org-article:07111a50d0a94ec8bdbd64b85d8570502021-11-14T12:18:16ZThree dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals10.1038/s41598-021-01528-42045-2322https://doaj.org/article/07111a50d0a94ec8bdbd64b85d8570502021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01528-4https://doaj.org/toc/2045-2322Abstract Neural recordings made to date through various approaches—both in-vitro or in-vivo—lack high spatial resolution and a high signal-to-noise ratio (SNR) required for detailed understanding of brain function, synaptic plasticity, and dysfunction. These shortcomings in turn deter the ability to further design diagnostic, therapeutic strategies and the fabrication of neuro-modulatory devices with various feedback loop systems. We report here on the simulation and fabrication of fully configurable neural micro-electrodes that can be used for both in vitro and in vivo applications, with three-dimensional semi-insulated structures patterned onto custom, fine-pitch, high density arrays. These microelectrodes were interfaced with isolated brain slices as well as implanted in brains of freely behaving rats to demonstrate their ability to maintain a high SNR. Moreover, the electrodes enabled the detection of epileptiform events and high frequency oscillations in an epilepsy model thus offering a diagnostic potential for neurological disorders such as epilepsy. These microelectrodes provide unique opportunities to study brain activity under normal and various pathological conditions, both in-vivo and in in-vitro, thus furthering the ability to develop drug screening and neuromodulation systems that could accurately record and map the activity of large neural networks over an extended time period.P. WijdenesK. HaiderC. GavriloviciB. GunningM. D. WolffT. LijnseR. ArmstrongG. C. TeskeyJ. M. RhoC. DaltonNaweed I. SyedNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q P. Wijdenes K. Haider C. Gavrilovici B. Gunning M. D. Wolff T. Lijnse R. Armstrong G. C. Teskey J. M. Rho C. Dalton Naweed I. Syed Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals
description	Abstract Neural recordings made to date through various approaches—both in-vitro or in-vivo—lack high spatial resolution and a high signal-to-noise ratio (SNR) required for detailed understanding of brain function, synaptic plasticity, and dysfunction. These shortcomings in turn deter the ability to further design diagnostic, therapeutic strategies and the fabrication of neuro-modulatory devices with various feedback loop systems. We report here on the simulation and fabrication of fully configurable neural micro-electrodes that can be used for both in vitro and in vivo applications, with three-dimensional semi-insulated structures patterned onto custom, fine-pitch, high density arrays. These microelectrodes were interfaced with isolated brain slices as well as implanted in brains of freely behaving rats to demonstrate their ability to maintain a high SNR. Moreover, the electrodes enabled the detection of epileptiform events and high frequency oscillations in an epilepsy model thus offering a diagnostic potential for neurological disorders such as epilepsy. These microelectrodes provide unique opportunities to study brain activity under normal and various pathological conditions, both in-vivo and in in-vitro, thus furthering the ability to develop drug screening and neuromodulation systems that could accurately record and map the activity of large neural networks over an extended time period.
format	article
author	P. Wijdenes K. Haider C. Gavrilovici B. Gunning M. D. Wolff T. Lijnse R. Armstrong G. C. Teskey J. M. Rho C. Dalton Naweed I. Syed
author_facet	P. Wijdenes K. Haider C. Gavrilovici B. Gunning M. D. Wolff T. Lijnse R. Armstrong G. C. Teskey J. M. Rho C. Dalton Naweed I. Syed
author_sort	P. Wijdenes
title	Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals
title_short	Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals
title_full	Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals
title_fullStr	Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals
title_full_unstemmed	Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals
title_sort	three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/07111a50d0a94ec8bdbd64b85d857050
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Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals

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