Functional and gene expression analysis of hTERT overexpressed endothelial cells

Haruna Takano1, Satoshi Murasawa1,2, Takayuki Asahara1,2,31Institute of Biomedical Research and Innovation, Kobe, Japan; 2RIKEN Center for Developmental Biology, Kobe 650-0047, Japan; 3Tokai University of School of Medicine, Tokai, JapanAbstract: Telomerase dysfunction contributes to cellular senesc...

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Autores principales:	Haruna Takano, Satoshi Murasawa, Takayuki Asahara
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0719a998d4d44e5394c37bac85fb0992
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spelling	oai:doaj.org-article:0719a998d4d44e5394c37bac85fb09922021-12-02T00:27:03ZFunctional and gene expression analysis of hTERT overexpressed endothelial cells1177-54751177-5491https://doaj.org/article/0719a998d4d44e5394c37bac85fb09922008-09-01T00:00:00Zhttp://www.dovepress.com/functional-and-gene-expression-analysis-of-htert-overexpressed-endothe-a2303https://doaj.org/toc/1177-5475https://doaj.org/toc/1177-5491Haruna Takano1, Satoshi Murasawa1,2, Takayuki Asahara1,2,31Institute of Biomedical Research and Innovation, Kobe, Japan; 2RIKEN Center for Developmental Biology, Kobe 650-0047, Japan; 3Tokai University of School of Medicine, Tokai, JapanAbstract: Telomerase dysfunction contributes to cellular senescence. Recent advances indicate the importance of senescence in maintaining vascular cell function in vitro. Human telomerase reverse transcriptase (hTERT) overexpression is thought to lead to resistance to apoptosis and oxidative stress. However, the mechanism in endothelial lineage cells is unclear. We tried to generate an immortal endothelial cell line from human umbilical vein endothelial cells using a no-virus system and examine the functional mechanisms of hTERT overexpressed endothelial cell senescence in vitro. High levels of hTERT genes and endothelial cell-specific markers were expressed during long-term culture. Also, angiogenic responses were observed in hTERT overexpressed endothelial cell. These cells showed a delay in senescence and appeared more resistant to stressed conditions. PI3K/Akt-related gene levels were enhanced in hTERT overexpressed endothelial cells. An up-regulated PI3K/Akt pathway caused by hTERT overexpression might contribute to anti-apoptosis and survival effects in endothelial lineage cells.Keywords: endothelial, telomerase, senescence, oxidative stress, anti-apoptosis, PI3K/Akt pathway Haruna TakanoSatoshi MurasawaTakayuki AsaharaDove Medical PressarticleMedicine (General)R5-920ENBiologics: Targets & Therapy, Vol 2008, Iss Issue 3, Pp 547-554 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Haruna Takano Satoshi Murasawa Takayuki Asahara Functional and gene expression analysis of hTERT overexpressed endothelial cells
description	Haruna Takano1, Satoshi Murasawa1,2, Takayuki Asahara1,2,31Institute of Biomedical Research and Innovation, Kobe, Japan; 2RIKEN Center for Developmental Biology, Kobe 650-0047, Japan; 3Tokai University of School of Medicine, Tokai, JapanAbstract: Telomerase dysfunction contributes to cellular senescence. Recent advances indicate the importance of senescence in maintaining vascular cell function in vitro. Human telomerase reverse transcriptase (hTERT) overexpression is thought to lead to resistance to apoptosis and oxidative stress. However, the mechanism in endothelial lineage cells is unclear. We tried to generate an immortal endothelial cell line from human umbilical vein endothelial cells using a no-virus system and examine the functional mechanisms of hTERT overexpressed endothelial cell senescence in vitro. High levels of hTERT genes and endothelial cell-specific markers were expressed during long-term culture. Also, angiogenic responses were observed in hTERT overexpressed endothelial cell. These cells showed a delay in senescence and appeared more resistant to stressed conditions. PI3K/Akt-related gene levels were enhanced in hTERT overexpressed endothelial cells. An up-regulated PI3K/Akt pathway caused by hTERT overexpression might contribute to anti-apoptosis and survival effects in endothelial lineage cells.Keywords: endothelial, telomerase, senescence, oxidative stress, anti-apoptosis, PI3K/Akt pathway
format	article
author	Haruna Takano Satoshi Murasawa Takayuki Asahara
author_facet	Haruna Takano Satoshi Murasawa Takayuki Asahara
author_sort	Haruna Takano
title	Functional and gene expression analysis of hTERT overexpressed endothelial cells
title_short	Functional and gene expression analysis of hTERT overexpressed endothelial cells
title_full	Functional and gene expression analysis of hTERT overexpressed endothelial cells
title_fullStr	Functional and gene expression analysis of hTERT overexpressed endothelial cells
title_full_unstemmed	Functional and gene expression analysis of hTERT overexpressed endothelial cells
title_sort	functional and gene expression analysis of htert overexpressed endothelial cells
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/0719a998d4d44e5394c37bac85fb0992
work_keys_str_mv	AT harunatakano functionalandgeneexpressionanalysisofhtertoverexpressedendothelialcells AT satoshimurasawa functionalandgeneexpressionanalysisofhtertoverexpressedendothelialcells AT takayukiasahara functionalandgeneexpressionanalysisofhtertoverexpressedendothelialcells
_version_	1718403719600537600

Functional and gene expression analysis of hTERT overexpressed endothelial cells

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