Comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis

Objective: To evaluate five examination techniques in the diagnosis and treatment of osteoarticular tuberculosis (TB). Methods: Microbiological samples were collected from a total of 284 patients during the period August 2017 to December 2019 in Wuhan Pulmonary Hospital. The specimens were examined...

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Autores principales:	Qibin Liu, Xianxiang Chen, Xiyong Dai, Xiaoyu Liu, Feng Xu, Peng Peng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Osteoarticular tuberculosis Nucleic acid amplification Gene Xpert MTB/RIF Etiological diagnosis Evaluation Drug resistance Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/071f6d3ce42a4a71a66dbaf2cee90692
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spelling	oai:doaj.org-article:071f6d3ce42a4a71a66dbaf2cee906922021-11-30T04:14:12ZComparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis1201-971210.1016/j.ijid.2021.09.019https://doaj.org/article/071f6d3ce42a4a71a66dbaf2cee906922021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S120197122100727Xhttps://doaj.org/toc/1201-9712Objective: To evaluate five examination techniques in the diagnosis and treatment of osteoarticular tuberculosis (TB). Methods: Microbiological samples were collected from a total of 284 patients during the period August 2017 to December 2019 in Wuhan Pulmonary Hospital. The specimens were examined by acid-fast bacillus (AFB) smear microscopy, mycobacterial culture, PCR, T-SPOT.TB, and X-pert MTB/RIF rapid molecular detection. Results: The diagnostic sensitivity of the Xpert technology was 96.8% (116/120), specificity was 96.8% (58/60), the Youden index was 0.936, and the area under the receiver operating characteristic (ROC) curve was 0.967. The sensitivity and specificity of PCR were 84.2% (104/128) and 95.2% (76/80), respectively; the area under the ROC curve was 0.881. T-SPOT.TB had a detection sensitivity of 75.0% (12/16) and specificity of 85.0% (17/20). AFB smear microscopy had a sensitivity of 60.0% (75/125) and specificity of 95.8% (152/159). TB culture sensitivity was 58.1% (72/124) and specificity was 96.2% (73/76). The sensitivity and specificity of Xpert MTB/RIF for detecting rifampicin resistance were 100% (2/2) and 97.3% (73/75), respectively. Conclusions: The Xpert MTB/RIF technique was found to have a good diagnostic value. With an additional diagnosis of Rifampicin resistance, it was also useful in tuberculosis therapy.Qibin LiuXianxiang ChenXiyong DaiXiaoyu LiuFeng XuPeng PengElsevierarticleOsteoarticular tuberculosisNucleic acid amplificationGene Xpert MTB/RIFEtiological diagnosisEvaluationDrug resistanceInfectious and parasitic diseasesRC109-216ENInternational Journal of Infectious Diseases, Vol 112, Iss , Pp 258-263 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteoarticular tuberculosis Nucleic acid amplification Gene Xpert MTB/RIF Etiological diagnosis Evaluation Drug resistance Infectious and parasitic diseases RC109-216
spellingShingle	Osteoarticular tuberculosis Nucleic acid amplification Gene Xpert MTB/RIF Etiological diagnosis Evaluation Drug resistance Infectious and parasitic diseases RC109-216 Qibin Liu Xianxiang Chen Xiyong Dai Xiaoyu Liu Feng Xu Peng Peng Comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis
description	Objective: To evaluate five examination techniques in the diagnosis and treatment of osteoarticular tuberculosis (TB). Methods: Microbiological samples were collected from a total of 284 patients during the period August 2017 to December 2019 in Wuhan Pulmonary Hospital. The specimens were examined by acid-fast bacillus (AFB) smear microscopy, mycobacterial culture, PCR, T-SPOT.TB, and X-pert MTB/RIF rapid molecular detection. Results: The diagnostic sensitivity of the Xpert technology was 96.8% (116/120), specificity was 96.8% (58/60), the Youden index was 0.936, and the area under the receiver operating characteristic (ROC) curve was 0.967. The sensitivity and specificity of PCR were 84.2% (104/128) and 95.2% (76/80), respectively; the area under the ROC curve was 0.881. T-SPOT.TB had a detection sensitivity of 75.0% (12/16) and specificity of 85.0% (17/20). AFB smear microscopy had a sensitivity of 60.0% (75/125) and specificity of 95.8% (152/159). TB culture sensitivity was 58.1% (72/124) and specificity was 96.2% (73/76). The sensitivity and specificity of Xpert MTB/RIF for detecting rifampicin resistance were 100% (2/2) and 97.3% (73/75), respectively. Conclusions: The Xpert MTB/RIF technique was found to have a good diagnostic value. With an additional diagnosis of Rifampicin resistance, it was also useful in tuberculosis therapy.
format	article
author	Qibin Liu Xianxiang Chen Xiyong Dai Xiaoyu Liu Feng Xu Peng Peng
author_facet	Qibin Liu Xianxiang Chen Xiyong Dai Xiaoyu Liu Feng Xu Peng Peng
author_sort	Qibin Liu
title	Comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis
title_short	Comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis
title_full	Comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis
title_fullStr	Comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis
title_full_unstemmed	Comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis
title_sort	comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/071f6d3ce42a4a71a66dbaf2cee90692
work_keys_str_mv	AT qibinliu comparativeanalysisoffiveinspectiontechniquesfortheapplicationinthediagnosisandtreatmentofosteoarticulartuberculosis AT xianxiangchen comparativeanalysisoffiveinspectiontechniquesfortheapplicationinthediagnosisandtreatmentofosteoarticulartuberculosis AT xiyongdai comparativeanalysisoffiveinspectiontechniquesfortheapplicationinthediagnosisandtreatmentofosteoarticulartuberculosis AT xiaoyuliu comparativeanalysisoffiveinspectiontechniquesfortheapplicationinthediagnosisandtreatmentofosteoarticulartuberculosis AT fengxu comparativeanalysisoffiveinspectiontechniquesfortheapplicationinthediagnosisandtreatmentofosteoarticulartuberculosis AT pengpeng comparativeanalysisoffiveinspectiontechniquesfortheapplicationinthediagnosisandtreatmentofosteoarticulartuberculosis
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Comparative analysis of five inspection techniques for the application in the diagnosis and treatment of osteoarticular tuberculosis

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