Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China

ABSTRACT: Duck adenoviruses (DAdVs) include serotype 1 (DAdV-1) in the genus Atadenovirus and serotypes 2-4 (DAdV-2, 3, and 4) in the genus Aviadenovirus. DAdV-3 was initially isolated from Chinese Muscovy ducks in 2014, whereby the infected ducks exhibited yellowing and hemorrhaging in the liver, a...

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Autores principales:	Xinjin Shi, Xinyu Zhang, Haiwei Sun, Changqing Wei, Yingnan Liu, Jiguan Luo, Xuebo Wang, Zongyan Chen, Hongjun Chen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	duck adenovirus 3 isolation pathogenicity mutant duck Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/07228134b1754e549ef8649246f97760
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spelling	oai:doaj.org-article:07228134b1754e549ef8649246f977602021-11-24T04:23:23ZIsolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China0032-579110.1016/j.psj.2021.101564https://doaj.org/article/07228134b1754e549ef8649246f977602022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005861https://doaj.org/toc/0032-5791ABSTRACT: Duck adenoviruses (DAdVs) include serotype 1 (DAdV-1) in the genus Atadenovirus and serotypes 2-4 (DAdV-2, 3, and 4) in the genus Aviadenovirus. DAdV-3 was initially isolated from Chinese Muscovy ducks in 2014, whereby the infected ducks exhibited yellowing and hemorrhaging in the liver, along with slight pericardial effusion, swelling, and hemorrhaging in the kidneys. In recent years, duck adenovirus infections have appeared in Muscovy duck farms in Fujian, Zhejiang, Anhui, Guangdong, and other places in China. They have an incidence rate of 40 to 55% and a mortality rate of 35 to 43%, resulting in great losses to the duck breeding industry. In this study, 7 DAdV-3 strains, designated as TZ193, FJPT20161124, GX20170519, FJZZ, GDMM, AHAQ, and GDHS were isolated from Muscovy ducks in different provinces of China during 2016–2019, and their complete genomics were sequenced. Their genomes all exhibited significant deletions in ORF67, which also had G to A transitions at the 41st and 977th nt positions, resulting in a stop codon. The pathogenicity of TZ193, a novel isolate of DAdV-3, was investigated in Muscovy ducks. TZ193 caused characteristic lesions of swelling as well as hemorrhagic liver and kidney in the infected ducklings. Moreover, the mortality rate of TZ193 in 5-day-old domestic ducks was 100%. Our data provide concrete evidence for the identification of the DAdV-3 novel variant mutant in China, which effects increased mortality in ducks. This highlights the necessity for monitoring the specific molecular epidemiology of novel DAdV-3 mutants and the development of new vaccines in the future.Xinjin ShiXinyu ZhangHaiwei SunChangqing WeiYingnan LiuJiguan LuoXuebo WangZongyan ChenHongjun ChenElsevierarticleduck adenovirus 3isolationpathogenicitymutantduckAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101564- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	duck adenovirus 3 isolation pathogenicity mutant duck Animal culture SF1-1100
spellingShingle	duck adenovirus 3 isolation pathogenicity mutant duck Animal culture SF1-1100 Xinjin Shi Xinyu Zhang Haiwei Sun Changqing Wei Yingnan Liu Jiguan Luo Xuebo Wang Zongyan Chen Hongjun Chen Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China
description	ABSTRACT: Duck adenoviruses (DAdVs) include serotype 1 (DAdV-1) in the genus Atadenovirus and serotypes 2-4 (DAdV-2, 3, and 4) in the genus Aviadenovirus. DAdV-3 was initially isolated from Chinese Muscovy ducks in 2014, whereby the infected ducks exhibited yellowing and hemorrhaging in the liver, along with slight pericardial effusion, swelling, and hemorrhaging in the kidneys. In recent years, duck adenovirus infections have appeared in Muscovy duck farms in Fujian, Zhejiang, Anhui, Guangdong, and other places in China. They have an incidence rate of 40 to 55% and a mortality rate of 35 to 43%, resulting in great losses to the duck breeding industry. In this study, 7 DAdV-3 strains, designated as TZ193, FJPT20161124, GX20170519, FJZZ, GDMM, AHAQ, and GDHS were isolated from Muscovy ducks in different provinces of China during 2016–2019, and their complete genomics were sequenced. Their genomes all exhibited significant deletions in ORF67, which also had G to A transitions at the 41st and 977th nt positions, resulting in a stop codon. The pathogenicity of TZ193, a novel isolate of DAdV-3, was investigated in Muscovy ducks. TZ193 caused characteristic lesions of swelling as well as hemorrhagic liver and kidney in the infected ducklings. Moreover, the mortality rate of TZ193 in 5-day-old domestic ducks was 100%. Our data provide concrete evidence for the identification of the DAdV-3 novel variant mutant in China, which effects increased mortality in ducks. This highlights the necessity for monitoring the specific molecular epidemiology of novel DAdV-3 mutants and the development of new vaccines in the future.
format	article
author	Xinjin Shi Xinyu Zhang Haiwei Sun Changqing Wei Yingnan Liu Jiguan Luo Xuebo Wang Zongyan Chen Hongjun Chen
author_facet	Xinjin Shi Xinyu Zhang Haiwei Sun Changqing Wei Yingnan Liu Jiguan Luo Xuebo Wang Zongyan Chen Hongjun Chen
author_sort	Xinjin Shi
title	Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China
title_short	Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China
title_full	Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China
title_fullStr	Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China
title_full_unstemmed	Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China
title_sort	isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in china
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/07228134b1754e549ef8649246f97760
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_version_	1718416016076177408

Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China

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