Transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep

Abstract Transcranial application of pulsed low-intensity focused ultrasound (FUS) modulates the excitability of region-specific brain areas, and anesthetic confounders on brain activity warrant the evaluation of the technique in awake animals. We examined the neuromodulatory effects of FUS in unane...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hyun-Chul Kim, Wonhye Lee, Jennifer Kunes, Kyungho Yoon, Ji Eun Lee, Lori Foley, Kavin Kowsari, Seung-Schik Yoo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0722839e36cc4693a5c1089ffa09083c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0722839e36cc4693a5c1089ffa09083c
record_format	dspace
spelling	oai:doaj.org-article:0722839e36cc4693a5c1089ffa09083c2021-12-02T19:17:04ZTranscranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep10.1038/s41598-021-98920-x2045-2322https://doaj.org/article/0722839e36cc4693a5c1089ffa09083c2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98920-xhttps://doaj.org/toc/2045-2322Abstract Transcranial application of pulsed low-intensity focused ultrasound (FUS) modulates the excitability of region-specific brain areas, and anesthetic confounders on brain activity warrant the evaluation of the technique in awake animals. We examined the neuromodulatory effects of FUS in unanesthetized sheep by developing a custom-fit headgear capable of reproducibly placing an acoustic focus on the unilateral motor cortex (M1) and corresponding thalamic area. The efferent responses to sonication, based on the acoustic parameters previously identified in anesthetized sheep, were measured using electromyography (EMG) from both hind limbs across three experimental conditions: on-target sonication, off-target sonication, and without sonication. Excitatory sonication yielded greater amplitude of EMG signals obtained from the hind limb contralateral to sonication than that from the ipsilateral limb. Spurious appearance of motion-related EMG signals limited the amount of analyzed data (~ 10% selection of acquired data) during excitatory sonication, and the averaged EMG response rates elicited by the M1 and thalamic stimulations were 7.5 ± 1.4% and 6.7 ± 1.5%, respectively. Suppressive sonication, while sheep walked on the treadmill, temporarily reduced the EMG amplitude from the limb contralateral to sonication. No significant change was found in the EMG amplitudes during the off-target sonication. Behavioral observation throughout the study and histological analysis showed no sign of brain tissue damage caused by the acoustic stimulation. Marginal response rates observed during excitatory sonication call for technical refinement to reduce motion artifacts during EMG acquisitions as well as acoustic aberration correction schemes to improve spatial accuracy of sonication. Yet, our results indicate that low-intensity FUS modulated the excitability of regional brain tissues reversibly and safely in awake sheep, supporting its potential in theragnostic applications.Hyun-Chul KimWonhye LeeJennifer KunesKyungho YoonJi Eun LeeLori FoleyKavin KowsariSeung-Schik YooNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyun-Chul Kim Wonhye Lee Jennifer Kunes Kyungho Yoon Ji Eun Lee Lori Foley Kavin Kowsari Seung-Schik Yoo Transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep
description	Abstract Transcranial application of pulsed low-intensity focused ultrasound (FUS) modulates the excitability of region-specific brain areas, and anesthetic confounders on brain activity warrant the evaluation of the technique in awake animals. We examined the neuromodulatory effects of FUS in unanesthetized sheep by developing a custom-fit headgear capable of reproducibly placing an acoustic focus on the unilateral motor cortex (M1) and corresponding thalamic area. The efferent responses to sonication, based on the acoustic parameters previously identified in anesthetized sheep, were measured using electromyography (EMG) from both hind limbs across three experimental conditions: on-target sonication, off-target sonication, and without sonication. Excitatory sonication yielded greater amplitude of EMG signals obtained from the hind limb contralateral to sonication than that from the ipsilateral limb. Spurious appearance of motion-related EMG signals limited the amount of analyzed data (~ 10% selection of acquired data) during excitatory sonication, and the averaged EMG response rates elicited by the M1 and thalamic stimulations were 7.5 ± 1.4% and 6.7 ± 1.5%, respectively. Suppressive sonication, while sheep walked on the treadmill, temporarily reduced the EMG amplitude from the limb contralateral to sonication. No significant change was found in the EMG amplitudes during the off-target sonication. Behavioral observation throughout the study and histological analysis showed no sign of brain tissue damage caused by the acoustic stimulation. Marginal response rates observed during excitatory sonication call for technical refinement to reduce motion artifacts during EMG acquisitions as well as acoustic aberration correction schemes to improve spatial accuracy of sonication. Yet, our results indicate that low-intensity FUS modulated the excitability of regional brain tissues reversibly and safely in awake sheep, supporting its potential in theragnostic applications.
format	article
author	Hyun-Chul Kim Wonhye Lee Jennifer Kunes Kyungho Yoon Ji Eun Lee Lori Foley Kavin Kowsari Seung-Schik Yoo
author_facet	Hyun-Chul Kim Wonhye Lee Jennifer Kunes Kyungho Yoon Ji Eun Lee Lori Foley Kavin Kowsari Seung-Schik Yoo
author_sort	Hyun-Chul Kim
title	Transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep
title_short	Transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep
title_full	Transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep
title_fullStr	Transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep
title_full_unstemmed	Transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep
title_sort	transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0722839e36cc4693a5c1089ffa09083c
work_keys_str_mv	AT hyunchulkim transcranialfocusedultrasoundmodulatescorticalandthalamicmotoractivityinawakesheep AT wonhyelee transcranialfocusedultrasoundmodulatescorticalandthalamicmotoractivityinawakesheep AT jenniferkunes transcranialfocusedultrasoundmodulatescorticalandthalamicmotoractivityinawakesheep AT kyunghoyoon transcranialfocusedultrasoundmodulatescorticalandthalamicmotoractivityinawakesheep AT jieunlee transcranialfocusedultrasoundmodulatescorticalandthalamicmotoractivityinawakesheep AT lorifoley transcranialfocusedultrasoundmodulatescorticalandthalamicmotoractivityinawakesheep AT kavinkowsari transcranialfocusedultrasoundmodulatescorticalandthalamicmotoractivityinawakesheep AT seungschikyoo transcranialfocusedultrasoundmodulatescorticalandthalamicmotoractivityinawakesheep
_version_	1718376895530139648

Transcranial focused ultrasound modulates cortical and thalamic motor activity in awake sheep

Ejemplares similares