Preparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material

Touseef Amna1, M Shamshi Hassan2, Ki-Taek Nam2, Yang You Bing3, Nasser AM Barakat2, Myung-Seob Khil2, Hak Yong Kim1,21Center for Healthcare Technology Development, 2Department of Organic Materials and Fiber Engineering, Chonbuk National University, Jeonju, Korea; 3Animal Science and Technology Colle...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Amna T, Hassan MS, Nam KT, Bing YY, Barakat NAM, Khil MS, Kim HY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/07232c0ebd5e499db468124819e50eee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:07232c0ebd5e499db468124819e50eee
record_format	dspace
spelling	oai:doaj.org-article:07232c0ebd5e499db468124819e50eee2021-12-02T05:14:22ZPreparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material1176-91141178-2013https://doaj.org/article/07232c0ebd5e499db468124819e50eee2012-03-01T00:00:00Zhttp://www.dovepress.com/preparation-characterization-and-cytotoxicity-of-cptfe2o3-embedded-plg-a9578https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Touseef Amna1, M Shamshi Hassan2, Ki-Taek Nam2, Yang You Bing3, Nasser AM Barakat2, Myung-Seob Khil2, Hak Yong Kim1,21Center for Healthcare Technology Development, 2Department of Organic Materials and Fiber Engineering, Chonbuk National University, Jeonju, Korea; 3Animal Science and Technology College, Henan University of Science and Technology, Luoyang, ChinaAbstract: The aim of this study was to fabricate camptothecin/iron(III) oxide (CPT/Fe2O3)-loaded poly(D,L-lactide-co-glycolide) (PLGA) composite mats to modulate the CPT release and to improve the structural integrity and antitumor activity of the released drug. The CPT/ Fe2O3-loaded PLGA ultrafine fibers were prepared for the first time by electrospinning a composite solution of CPT/Fe2O3 and neat PLGA (4 weight percent). The physicochemical characterization of the electrospun composite mat was carried out by scanning electron microscopy, energy dispersive X-ray spectroscopy, electron probe microanalysis, thermogravimetry, transmission electron microscopy, ultraviolet-visible spectroscopy, and X-ray diffraction pattern. The medicated composite fibers were evaluated for their cytotoxicity on C2C12 cells using Cell Counting Kit-8 assay (Sigma-Aldrich Corporation, St Louis, MO). The in vitro studies indicated a slow and prolonged release over a period of 96 hours with mild initial burst. Scanning electron microscopy, thermogravimetry, and X-ray diffraction studies confirmed the interaction of CPT/Fe2O3 with the PLGA matrix and showed that the crystallinity of CPT decreased after loading. Incorporation of CPT in the polymer media affected both the morphology and the size of the CPT/Fe2O3-loaded PLGA composite fibers. Electron probe microanalysis and energy dispersive X-ray spectroscopy results confirmed well-oriented composite ultrafine fibers with good incorporation of CPT/Fe2O3. The cytotoxicity results illustrate that the pristine PLGA did not exhibit noteworthy cytotoxicity; conversely, the CPT/Fe2O3 composite fibers inhibited C2C12 cells significantly. Thus, the current work demonstrates that the CPT/Fe2O3-loaded PLGA composite fibers represent a promising chemotherapeutic system for enhancing anticancer drug efficacy and selectively targeting cancer cells in order to treat diverse cancers.Keywords: camptothecin, C2C12 cells, Fe2O3 nanoparticles, electrospinning, cytotoxicityAmna THassan MSNam KTBing YYBarakat NAMKhil MSKim HYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1659-1670 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Amna T Hassan MS Nam KT Bing YY Barakat NAM Khil MS Kim HY Preparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material
description	Touseef Amna1, M Shamshi Hassan2, Ki-Taek Nam2, Yang You Bing3, Nasser AM Barakat2, Myung-Seob Khil2, Hak Yong Kim1,21Center for Healthcare Technology Development, 2Department of Organic Materials and Fiber Engineering, Chonbuk National University, Jeonju, Korea; 3Animal Science and Technology College, Henan University of Science and Technology, Luoyang, ChinaAbstract: The aim of this study was to fabricate camptothecin/iron(III) oxide (CPT/Fe2O3)-loaded poly(D,L-lactide-co-glycolide) (PLGA) composite mats to modulate the CPT release and to improve the structural integrity and antitumor activity of the released drug. The CPT/ Fe2O3-loaded PLGA ultrafine fibers were prepared for the first time by electrospinning a composite solution of CPT/Fe2O3 and neat PLGA (4 weight percent). The physicochemical characterization of the electrospun composite mat was carried out by scanning electron microscopy, energy dispersive X-ray spectroscopy, electron probe microanalysis, thermogravimetry, transmission electron microscopy, ultraviolet-visible spectroscopy, and X-ray diffraction pattern. The medicated composite fibers were evaluated for their cytotoxicity on C2C12 cells using Cell Counting Kit-8 assay (Sigma-Aldrich Corporation, St Louis, MO). The in vitro studies indicated a slow and prolonged release over a period of 96 hours with mild initial burst. Scanning electron microscopy, thermogravimetry, and X-ray diffraction studies confirmed the interaction of CPT/Fe2O3 with the PLGA matrix and showed that the crystallinity of CPT decreased after loading. Incorporation of CPT in the polymer media affected both the morphology and the size of the CPT/Fe2O3-loaded PLGA composite fibers. Electron probe microanalysis and energy dispersive X-ray spectroscopy results confirmed well-oriented composite ultrafine fibers with good incorporation of CPT/Fe2O3. The cytotoxicity results illustrate that the pristine PLGA did not exhibit noteworthy cytotoxicity; conversely, the CPT/Fe2O3 composite fibers inhibited C2C12 cells significantly. Thus, the current work demonstrates that the CPT/Fe2O3-loaded PLGA composite fibers represent a promising chemotherapeutic system for enhancing anticancer drug efficacy and selectively targeting cancer cells in order to treat diverse cancers.Keywords: camptothecin, C2C12 cells, Fe2O3 nanoparticles, electrospinning, cytotoxicity
format	article
author	Amna T Hassan MS Nam KT Bing YY Barakat NAM Khil MS Kim HY
author_facet	Amna T Hassan MS Nam KT Bing YY Barakat NAM Khil MS Kim HY
author_sort	Amna T
title	Preparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material
title_short	Preparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material
title_full	Preparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material
title_fullStr	Preparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material
title_full_unstemmed	Preparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material
title_sort	preparation, characterization, and cytotoxicity of cpt/fe2o3-embedded plga ultrafine composite fibers: a synergistic approach to develop promising anticancer material
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/07232c0ebd5e499db468124819e50eee
work_keys_str_mv	AT amnat preparationcharacterizationandcytotoxicityofcptfe2o3embeddedplgaultrafinecompositefibersasynergisticapproachtodeveloppromisinganticancermaterial AT hassanms preparationcharacterizationandcytotoxicityofcptfe2o3embeddedplgaultrafinecompositefibersasynergisticapproachtodeveloppromisinganticancermaterial AT namkt preparationcharacterizationandcytotoxicityofcptfe2o3embeddedplgaultrafinecompositefibersasynergisticapproachtodeveloppromisinganticancermaterial AT bingyy preparationcharacterizationandcytotoxicityofcptfe2o3embeddedplgaultrafinecompositefibersasynergisticapproachtodeveloppromisinganticancermaterial AT barakatnam preparationcharacterizationandcytotoxicityofcptfe2o3embeddedplgaultrafinecompositefibersasynergisticapproachtodeveloppromisinganticancermaterial AT khilms preparationcharacterizationandcytotoxicityofcptfe2o3embeddedplgaultrafinecompositefibersasynergisticapproachtodeveloppromisinganticancermaterial AT kimhy preparationcharacterizationandcytotoxicityofcptfe2o3embeddedplgaultrafinecompositefibersasynergisticapproachtodeveloppromisinganticancermaterial
_version_	1718400453083922432

Preparation, characterization, and cytotoxicity of CPT/Fe2O3-embedded PLGA ultrafine composite fibers: a synergistic approach to develop promising anticancer material

Ejemplares similares