Cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip

Abstract This paper presents progress in the automation of cell and tissue systems and attempts toward the in situ feedback control of organs-on-a-chip. Our study aims to achieve feedback control of a cell and tissue system by a personal computer (PC), whereas most studies on organs-on-a-chip focus...

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Autores principales:	Satoshi Konishi, Takeshi Hashimoto, Tsubasa Nakabuchi, Takatoshi Ozeki, Hiroki Kajita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/072c0b0b83374574bbab51ba001bb83a
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spelling	oai:doaj.org-article:072c0b0b83374574bbab51ba001bb83a2021-12-02T14:06:55ZCell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip10.1038/s41598-020-80447-22045-2322https://doaj.org/article/072c0b0b83374574bbab51ba001bb83a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80447-2https://doaj.org/toc/2045-2322Abstract This paper presents progress in the automation of cell and tissue systems and attempts toward the in situ feedback control of organs-on-a-chip. Our study aims to achieve feedback control of a cell and tissue system by a personal computer (PC), whereas most studies on organs-on-a-chip focus on the automation of status monitoring. The implemented system is composed of subsystems including automated culture, stimulation, and monitoring. The monitoring function provides imaging as well as sampling and dispensing in combination with an external analyzer. Individual subsystems can be combined accordingly. First, monitoring of skeletal muscle (SM) and adipose tissues using this system was demonstrated. The highlight of this paper is the application of the system to the feedback control of the lipid droplet (LD) size, where biochemical stimulation using insulin and adrenaline is controlled by a PC according to the obtained LD imaging data. In this study, the system demonstrated its function of maintaining the desired size of LDs. Our results expand the possibility of PC-controllable cell and tissue systems by addressing the challenge of feedback control of organs-on-a-chip. The PC-controllable cell and tissue systems will contribute to living systems-on-a-chip based on homeostasis phenomena involving interactions between organs or tissues.Satoshi KonishiTakeshi HashimotoTsubasa NakabuchiTakatoshi OzekiHiroki KajitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoshi Konishi Takeshi Hashimoto Tsubasa Nakabuchi Takatoshi Ozeki Hiroki Kajita Cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip
description	Abstract This paper presents progress in the automation of cell and tissue systems and attempts toward the in situ feedback control of organs-on-a-chip. Our study aims to achieve feedback control of a cell and tissue system by a personal computer (PC), whereas most studies on organs-on-a-chip focus on the automation of status monitoring. The implemented system is composed of subsystems including automated culture, stimulation, and monitoring. The monitoring function provides imaging as well as sampling and dispensing in combination with an external analyzer. Individual subsystems can be combined accordingly. First, monitoring of skeletal muscle (SM) and adipose tissues using this system was demonstrated. The highlight of this paper is the application of the system to the feedback control of the lipid droplet (LD) size, where biochemical stimulation using insulin and adrenaline is controlled by a PC according to the obtained LD imaging data. In this study, the system demonstrated its function of maintaining the desired size of LDs. Our results expand the possibility of PC-controllable cell and tissue systems by addressing the challenge of feedback control of organs-on-a-chip. The PC-controllable cell and tissue systems will contribute to living systems-on-a-chip based on homeostasis phenomena involving interactions between organs or tissues.
format	article
author	Satoshi Konishi Takeshi Hashimoto Tsubasa Nakabuchi Takatoshi Ozeki Hiroki Kajita
author_facet	Satoshi Konishi Takeshi Hashimoto Tsubasa Nakabuchi Takatoshi Ozeki Hiroki Kajita
author_sort	Satoshi Konishi
title	Cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip
title_short	Cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip
title_full	Cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip
title_fullStr	Cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip
title_full_unstemmed	Cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip
title_sort	cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/072c0b0b83374574bbab51ba001bb83a
work_keys_str_mv	AT satoshikonishi cellandtissuesystemcapableofautomatedculturestimulationandmonitorwiththeaimoffeedbackcontroloforgansonachip AT takeshihashimoto cellandtissuesystemcapableofautomatedculturestimulationandmonitorwiththeaimoffeedbackcontroloforgansonachip AT tsubasanakabuchi cellandtissuesystemcapableofautomatedculturestimulationandmonitorwiththeaimoffeedbackcontroloforgansonachip AT takatoshiozeki cellandtissuesystemcapableofautomatedculturestimulationandmonitorwiththeaimoffeedbackcontroloforgansonachip AT hirokikajita cellandtissuesystemcapableofautomatedculturestimulationandmonitorwiththeaimoffeedbackcontroloforgansonachip
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Cell and tissue system capable of automated culture, stimulation, and monitor with the aim of feedback control of organs-on-a-chip

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