Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition

Zymomonas mobilis, a promising candidate for industrial biofuel production, is capable of nitrogen fixation naturally without hindering ethanol production. However, little is known about the regulation of nitrogen fixation in Z. mobilis. We herein conducted a high throughput analysis of proteome and...

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Autores principales:	Ayesha Nisar, Xiangxu Gongye, Yuhuan Huang, Sawar Khan, Mao Chen, Bo Wu, Mingxiong He
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Zymomonas mobilis 4D label-free proteomics nitrogen fixation post-translational modification lysine acetylome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/07380f5c30c6475696f71c261cc41bec
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spelling	oai:doaj.org-article:07380f5c30c6475696f71c261cc41bec2021-11-04T10:47:14ZGenome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition1664-302X10.3389/fmicb.2021.740555https://doaj.org/article/07380f5c30c6475696f71c261cc41bec2021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.740555/fullhttps://doaj.org/toc/1664-302XZymomonas mobilis, a promising candidate for industrial biofuel production, is capable of nitrogen fixation naturally without hindering ethanol production. However, little is known about the regulation of nitrogen fixation in Z. mobilis. We herein conducted a high throughput analysis of proteome and protein acetylation in Z. mobilis under N2-fixing conditions and established its first acetylome. The upregulated proteins mainly belong to processes of nitrogen fixation, motility, chemotaxis, flagellar assembly, energy production, transportation, and oxidation–reduction. Whereas, downregulated proteins are mainly related to energy-consuming and biosynthetic processes. Our acetylome analyses revealed 197 uniquely acetylated proteins, belonging to major pathways such as nitrogen fixation, central carbon metabolism, ammonia assimilation pathway, protein biosynthesis, and amino acid metabolism. Further, we observed acetylation in glycolytic enzymes of central carbon metabolism, the nitrogenase complex, the master regulator NifA, and the enzyme in GS/GOGAT cycle. These findings suggest that protein acetylation may play an important role in regulating various aspects of N2-metabolism in Z. mobilis. This study provides new knowledge of specific proteins and their associated cellular processes and pathways that may be regulated by protein acetylation in Z. mobilis.Ayesha NisarAyesha NisarXiangxu GongyeYuhuan HuangYuhuan HuangSawar KhanMao ChenMao ChenBo WuMingxiong HeFrontiers Media S.A.articleZymomonas mobilis4D label-free proteomicsnitrogen fixationpost-translational modificationlysine acetylomeMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zymomonas mobilis 4D label-free proteomics nitrogen fixation post-translational modification lysine acetylome Microbiology QR1-502
spellingShingle	Zymomonas mobilis 4D label-free proteomics nitrogen fixation post-translational modification lysine acetylome Microbiology QR1-502 Ayesha Nisar Ayesha Nisar Xiangxu Gongye Yuhuan Huang Yuhuan Huang Sawar Khan Mao Chen Mao Chen Bo Wu Mingxiong He Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition
description	Zymomonas mobilis, a promising candidate for industrial biofuel production, is capable of nitrogen fixation naturally without hindering ethanol production. However, little is known about the regulation of nitrogen fixation in Z. mobilis. We herein conducted a high throughput analysis of proteome and protein acetylation in Z. mobilis under N2-fixing conditions and established its first acetylome. The upregulated proteins mainly belong to processes of nitrogen fixation, motility, chemotaxis, flagellar assembly, energy production, transportation, and oxidation–reduction. Whereas, downregulated proteins are mainly related to energy-consuming and biosynthetic processes. Our acetylome analyses revealed 197 uniquely acetylated proteins, belonging to major pathways such as nitrogen fixation, central carbon metabolism, ammonia assimilation pathway, protein biosynthesis, and amino acid metabolism. Further, we observed acetylation in glycolytic enzymes of central carbon metabolism, the nitrogenase complex, the master regulator NifA, and the enzyme in GS/GOGAT cycle. These findings suggest that protein acetylation may play an important role in regulating various aspects of N2-metabolism in Z. mobilis. This study provides new knowledge of specific proteins and their associated cellular processes and pathways that may be regulated by protein acetylation in Z. mobilis.
format	article
author	Ayesha Nisar Ayesha Nisar Xiangxu Gongye Yuhuan Huang Yuhuan Huang Sawar Khan Mao Chen Mao Chen Bo Wu Mingxiong He
author_facet	Ayesha Nisar Ayesha Nisar Xiangxu Gongye Yuhuan Huang Yuhuan Huang Sawar Khan Mao Chen Mao Chen Bo Wu Mingxiong He
author_sort	Ayesha Nisar
title	Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition
title_short	Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition
title_full	Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition
title_fullStr	Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition
title_full_unstemmed	Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition
title_sort	genome-wide analyses of proteome and acetylome in zymomonas mobilis under n2-fixing condition
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/07380f5c30c6475696f71c261cc41bec
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Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition

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