Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody

Abstract For the sensitive diagnosis of colorectal cancer lesions, advanced molecular imaging techniques using cancer-specific targets have emerged. However, issues regarding the clearance of unbound probes and immunogenicity remain unresolved. To overcome these limitations, we developed a small-siz...

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Autores principales:	Hyung Il Kim, Jinhyeon Kim, Hyori Kim, Hyeri Lee, Yong Sik Yoon, Sung Wook Hwang, Sang Hyoung Park, Dong-Hoon Yang, Byong Duk Ye, Jeong-Sik Byeon, Suk-Kyun Yang, Sun Young Kim, Seung-Jae Myung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07439de36e144e6a9651897241ee385b
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spelling	oai:doaj.org-article:07439de36e144e6a9651897241ee385b2021-12-02T19:02:39ZBiomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody10.1038/s41598-021-96281-z2045-2322https://doaj.org/article/07439de36e144e6a9651897241ee385b2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96281-zhttps://doaj.org/toc/2045-2322Abstract For the sensitive diagnosis of colorectal cancer lesions, advanced molecular imaging techniques using cancer-specific targets have emerged. However, issues regarding the clearance of unbound probes and immunogenicity remain unresolved. To overcome these limitations, we developed a small-sized scFv antibody fragment conjugated with FITC for the real-time detection of colorectal cancer by in vivo molecular endoscopy imaging. A small-sized scFv fragment can target colon cancer secreted protein-2 (CCSP-2), highly expressed in colorectal adenocarcinoma tissues; moreover, its full-length IgG probe has been used for molecular imaging previously. To assess the efficacy of anti-CCSP-2 scFv-FITC, surgical specimens were obtained from 21 patients with colorectal cancer for ex vivo molecular fluorescence analysis, histology, and immunohistochemistry. Orthotopic mice were administered with anti-CCSP-2 scFv-FITC topically and intravenously, and distinct tumor lesions were observed by real-time fluorescence colonoscopy. The fluorescence imaging of human colon cancer specimens allowed the differentiation of malignant tissues from non-malignant tissues (p < 0.05), and the CCSP-2 expression level was found to be correlated with the fluorescence intensity. Here, we demonstrated the feasibility and safety of anti-CCSP-2 scFv-FITC for molecular imaging as well as its potential in real-time fluorescence colonoscopy for the differential diagnosis of tumor lesions.Hyung Il KimJinhyeon KimHyori KimHyeri LeeYong Sik YoonSung Wook HwangSang Hyoung ParkDong-Hoon YangByong Duk YeJeong-Sik ByeonSuk-Kyun YangSun Young KimSeung-Jae MyungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyung Il Kim Jinhyeon Kim Hyori Kim Hyeri Lee Yong Sik Yoon Sung Wook Hwang Sang Hyoung Park Dong-Hoon Yang Byong Duk Ye Jeong-Sik Byeon Suk-Kyun Yang Sun Young Kim Seung-Jae Myung Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody
description	Abstract For the sensitive diagnosis of colorectal cancer lesions, advanced molecular imaging techniques using cancer-specific targets have emerged. However, issues regarding the clearance of unbound probes and immunogenicity remain unresolved. To overcome these limitations, we developed a small-sized scFv antibody fragment conjugated with FITC for the real-time detection of colorectal cancer by in vivo molecular endoscopy imaging. A small-sized scFv fragment can target colon cancer secreted protein-2 (CCSP-2), highly expressed in colorectal adenocarcinoma tissues; moreover, its full-length IgG probe has been used for molecular imaging previously. To assess the efficacy of anti-CCSP-2 scFv-FITC, surgical specimens were obtained from 21 patients with colorectal cancer for ex vivo molecular fluorescence analysis, histology, and immunohistochemistry. Orthotopic mice were administered with anti-CCSP-2 scFv-FITC topically and intravenously, and distinct tumor lesions were observed by real-time fluorescence colonoscopy. The fluorescence imaging of human colon cancer specimens allowed the differentiation of malignant tissues from non-malignant tissues (p < 0.05), and the CCSP-2 expression level was found to be correlated with the fluorescence intensity. Here, we demonstrated the feasibility and safety of anti-CCSP-2 scFv-FITC for molecular imaging as well as its potential in real-time fluorescence colonoscopy for the differential diagnosis of tumor lesions.
format	article
author	Hyung Il Kim Jinhyeon Kim Hyori Kim Hyeri Lee Yong Sik Yoon Sung Wook Hwang Sang Hyoung Park Dong-Hoon Yang Byong Duk Ye Jeong-Sik Byeon Suk-Kyun Yang Sun Young Kim Seung-Jae Myung
author_facet	Hyung Il Kim Jinhyeon Kim Hyori Kim Hyeri Lee Yong Sik Yoon Sung Wook Hwang Sang Hyoung Park Dong-Hoon Yang Byong Duk Ye Jeong-Sik Byeon Suk-Kyun Yang Sun Young Kim Seung-Jae Myung
author_sort	Hyung Il Kim
title	Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody
title_short	Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody
title_full	Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody
title_fullStr	Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody
title_full_unstemmed	Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody
title_sort	biomolecular imaging of colorectal tumor lesions using a fitc-labeled scfv-cκ fragment antibody
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/07439de36e144e6a9651897241ee385b
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Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody

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