Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation

Abstract. Kartika EBA, Siregar LAM, Basyuni M. 2021. Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation. Biodiversitas 22: 3423-3436. Oil palm tissue culture is carried out through indirect embryogenesis, which causes somaclon...

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Autores principales:	Luthfi Aziz Mahmud Siregar, ERKY BEBY AYU KARTIKA, MOHAMMAD BASYUNI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	dolichol, oil palm, polyprenol, tissue culture Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/07451fa17df549eab78f257977a4fcf2
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spelling	oai:doaj.org-article:07451fa17df549eab78f257977a4fcf22021-11-22T12:14:18ZDistribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation1412-033X2085-472210.13057/biodiv/d220651https://doaj.org/article/07451fa17df549eab78f257977a4fcf22021-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8297https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Kartika EBA, Siregar LAM, Basyuni M. 2021. Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation. Biodiversitas 22: 3423-3436. Oil palm tissue culture is carried out through indirect embryogenesis, which causes somaclonal diversity to occur at the in vitro propagation stage, especially in the callus growth phase. In the cells of all living organisms can be found a group of polyisoprenoid compounds. This study aims to determine variations in oil palm plants resulting from tissue culture based on the presence of polyisoprenoid compounds. Oil palm leaf samples (Elaeis guineensis Jacq.) were collected from the plantation of PT. Socfin Indonesia, North Sumatra, Indonesia. Sample extraction, saponification, isolation of polyisoprenoid compounds, and two-dimensional thin-layer chromatography analysis were carried out to obtain data related to total lipids, polyprenol and dolichol profile, and Carbon (C) chain length polyprenol and dolichol from oil palm leaves of Pisifera parents and their propagated derivatives by indirect embryogenesis. The results showed that the amount of polyisoprenoid mother S24 was 2.41 mg/g higher than that of the parentS8 at 2.17 mg/g dry weight. Total polyisoprenoid fromS8 plants in vitro ranged from 0.71 - 8.53 mg/g dry weight, with the lowest total polyisoprenoid found atS894/22 and the highest was found atS893/2. While for total polyisoprenoid from plant tissue culture of Pisifera S24 ranged from 0.73 - 8.05 mg/g dry weight, with the lowest total polyisoprenoid found at S24H14 and the highest was found at S24H16. The parent plants of Pisifera S8 and S24, as well as plants resulting from tissue culture, were categorized as having lipid pattern type II, which showed a balanced distribution of polyprenol with dolichol. The longest carbon chain was found in vitro plantsS8 93/4 ranged from C50-C110, while the shortest was found in plants produced in vitro S24H7 starting from C45-C55. There were variations in the carbon chain length of polyprenol and dolichol in the leaf samples derived from in vitro propagation of the Pisifera S8 and S24 parents.Luthfi Aziz Mahmud SiregarERKY BEBY AYU KARTIKAMOHAMMAD BASYUNIMBI & UNS Soloarticledolichol, oil palm, polyprenol, tissue cultureBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dolichol, oil palm, polyprenol, tissue culture Biology (General) QH301-705.5
spellingShingle	dolichol, oil palm, polyprenol, tissue culture Biology (General) QH301-705.5 Luthfi Aziz Mahmud Siregar ERKY BEBY AYU KARTIKA MOHAMMAD BASYUNI Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation
description	Abstract. Kartika EBA, Siregar LAM, Basyuni M. 2021. Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation. Biodiversitas 22: 3423-3436. Oil palm tissue culture is carried out through indirect embryogenesis, which causes somaclonal diversity to occur at the in vitro propagation stage, especially in the callus growth phase. In the cells of all living organisms can be found a group of polyisoprenoid compounds. This study aims to determine variations in oil palm plants resulting from tissue culture based on the presence of polyisoprenoid compounds. Oil palm leaf samples (Elaeis guineensis Jacq.) were collected from the plantation of PT. Socfin Indonesia, North Sumatra, Indonesia. Sample extraction, saponification, isolation of polyisoprenoid compounds, and two-dimensional thin-layer chromatography analysis were carried out to obtain data related to total lipids, polyprenol and dolichol profile, and Carbon (C) chain length polyprenol and dolichol from oil palm leaves of Pisifera parents and their propagated derivatives by indirect embryogenesis. The results showed that the amount of polyisoprenoid mother S24 was 2.41 mg/g higher than that of the parentS8 at 2.17 mg/g dry weight. Total polyisoprenoid fromS8 plants in vitro ranged from 0.71 - 8.53 mg/g dry weight, with the lowest total polyisoprenoid found atS894/22 and the highest was found atS893/2. While for total polyisoprenoid from plant tissue culture of Pisifera S24 ranged from 0.73 - 8.05 mg/g dry weight, with the lowest total polyisoprenoid found at S24H14 and the highest was found at S24H16. The parent plants of Pisifera S8 and S24, as well as plants resulting from tissue culture, were categorized as having lipid pattern type II, which showed a balanced distribution of polyprenol with dolichol. The longest carbon chain was found in vitro plantsS8 93/4 ranged from C50-C110, while the shortest was found in plants produced in vitro S24H7 starting from C45-C55. There were variations in the carbon chain length of polyprenol and dolichol in the leaf samples derived from in vitro propagation of the Pisifera S8 and S24 parents.
format	article
author	Luthfi Aziz Mahmud Siregar ERKY BEBY AYU KARTIKA MOHAMMAD BASYUNI
author_facet	Luthfi Aziz Mahmud Siregar ERKY BEBY AYU KARTIKA MOHAMMAD BASYUNI
author_sort	Luthfi Aziz Mahmud Siregar
title	Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation
title_short	Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation
title_full	Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation
title_fullStr	Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation
title_full_unstemmed	Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation
title_sort	distribution of polyprenol and dolichol in oil palms from pisifera parents and mature plants from tissue culture propagation
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/07451fa17df549eab78f257977a4fcf2
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Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation

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