Up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].

Cutaneous malignant melanoma is the fastest increasing malignancy in humans. Hepatoma-derived growth factor (HDGF) is a novel growth factor identified from human hepatoma cell line. HDGF overexpression is correlated with poor prognosis in various types of cancer including melanoma. However, the unde...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Han-En Tsai, Jian-Ching Wu, Mei-Lang Kung, Li-Feng Liu, Lai-Hsin Kuo, Hsiao-Mei Kuo, San-Cher Chen, Elsa C Chan, Chieh-Shan Wu, Ming-Hong Tai, Guei-Sheung Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07456c78a88b4cd1ae7842ccf0b5476b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:07456c78a88b4cd1ae7842ccf0b5476b
record_format	dspace
spelling	oai:doaj.org-article:07456c78a88b4cd1ae7842ccf0b5476b2021-11-18T07:52:09ZUp-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].1932-620310.1371/journal.pone.0059345https://doaj.org/article/07456c78a88b4cd1ae7842ccf0b5476b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23536873/?tool=EBIhttps://doaj.org/toc/1932-6203Cutaneous malignant melanoma is the fastest increasing malignancy in humans. Hepatoma-derived growth factor (HDGF) is a novel growth factor identified from human hepatoma cell line. HDGF overexpression is correlated with poor prognosis in various types of cancer including melanoma. However, the underlying mechanism of HDGF overexpression in developing melanoma remains unclear. In this study, human melanoma cell lines (A375, A2058, MEL-RM and MM200) showed higher levels of HDGF gene expression, whereas human epidermal melanocytes (HEMn) expressed less. Exogenous application of HDGF stimulated colony formation and invasion of human melanoma cells. Moreover, HDGF overexpression stimulated the degree of invasion and colony formation of B16-F10 melanoma cells whereas HDGF knockdown exerted opposite effects in vitro. To evaluate the effects of HDGF on tumour growth and metastasis in vivo, syngeneic mouse melanoma and metastatic melanoma models were performed by manipulating the gene expression of HDGF in melanoma cells. It was found that mice injected with HDGF-overexpressing melanoma cells had greater tumour growth and higher metastatic capability. In contrast, mice implanted with HDGF-depleted melanoma cells exhibited reduced tumor burden and lung metastasis. Histological analysis of excised tumors revealed higher degree of cell proliferation and neovascularization in HDGF-overexpressing melanoma. The present study provides evidence that HDGF promotes tumor progression of melanoma and targeting HDGF may constitute a novel strategy for the treatment of melanoma.Han-En TsaiJian-Ching WuMei-Lang KungLi-Feng LiuLai-Hsin KuoHsiao-Mei KuoSan-Cher ChenElsa C ChanChieh-Shan WuMing-Hong TaiGuei-Sheung LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59345 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Han-En Tsai Jian-Ching Wu Mei-Lang Kung Li-Feng Liu Lai-Hsin Kuo Hsiao-Mei Kuo San-Cher Chen Elsa C Chan Chieh-Shan Wu Ming-Hong Tai Guei-Sheung Liu Up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].
description	Cutaneous malignant melanoma is the fastest increasing malignancy in humans. Hepatoma-derived growth factor (HDGF) is a novel growth factor identified from human hepatoma cell line. HDGF overexpression is correlated with poor prognosis in various types of cancer including melanoma. However, the underlying mechanism of HDGF overexpression in developing melanoma remains unclear. In this study, human melanoma cell lines (A375, A2058, MEL-RM and MM200) showed higher levels of HDGF gene expression, whereas human epidermal melanocytes (HEMn) expressed less. Exogenous application of HDGF stimulated colony formation and invasion of human melanoma cells. Moreover, HDGF overexpression stimulated the degree of invasion and colony formation of B16-F10 melanoma cells whereas HDGF knockdown exerted opposite effects in vitro. To evaluate the effects of HDGF on tumour growth and metastasis in vivo, syngeneic mouse melanoma and metastatic melanoma models were performed by manipulating the gene expression of HDGF in melanoma cells. It was found that mice injected with HDGF-overexpressing melanoma cells had greater tumour growth and higher metastatic capability. In contrast, mice implanted with HDGF-depleted melanoma cells exhibited reduced tumor burden and lung metastasis. Histological analysis of excised tumors revealed higher degree of cell proliferation and neovascularization in HDGF-overexpressing melanoma. The present study provides evidence that HDGF promotes tumor progression of melanoma and targeting HDGF may constitute a novel strategy for the treatment of melanoma.
format	article
author	Han-En Tsai Jian-Ching Wu Mei-Lang Kung Li-Feng Liu Lai-Hsin Kuo Hsiao-Mei Kuo San-Cher Chen Elsa C Chan Chieh-Shan Wu Ming-Hong Tai Guei-Sheung Liu
author_facet	Han-En Tsai Jian-Ching Wu Mei-Lang Kung Li-Feng Liu Lai-Hsin Kuo Hsiao-Mei Kuo San-Cher Chen Elsa C Chan Chieh-Shan Wu Ming-Hong Tai Guei-Sheung Liu
author_sort	Han-En Tsai
title	Up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].
title_short	Up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].
title_full	Up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].
title_fullStr	Up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].
title_full_unstemmed	Up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].
title_sort	up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/07456c78a88b4cd1ae7842ccf0b5476b
work_keys_str_mv	AT hanentsai upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT jianchingwu upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT meilangkung upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT lifengliu upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT laihsinkuo upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT hsiaomeikuo upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT sancherchen upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT elsacchan upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT chiehshanwu upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT minghongtai upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected AT gueisheungliu upregulationofhepatomaderivedgrowthfactorfacilitatestumorprogressioninmalignantmelanomacorrected
_version_	1718422831418572800

Up-regulation of hepatoma-derived growth factor facilitates tumor progression in malignant melanoma [corrected].

Ejemplares similares