Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.

Coix lacryma-jobi L. is a very important economic crop widely cultivated in Southeast Asia. Drought affects more than four million square kilometers every year, and is a significant factor limiting agricultural productivity. However, relatively little is known about how Coix lacryma-jobi L. responds...

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Autores principales:	Guidong Miao, Yan Qin, Jihua Guo, Qingxia Zhang, Yingying Bao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0750f287027b41d3aa43d6a24b2ee6c7
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spelling	oai:doaj.org-article:0750f287027b41d3aa43d6a24b2ee6c72021-12-02T20:14:49ZTranscriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.1932-620310.1371/journal.pone.0256875https://doaj.org/article/0750f287027b41d3aa43d6a24b2ee6c72021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256875https://doaj.org/toc/1932-6203Coix lacryma-jobi L. is a very important economic crop widely cultivated in Southeast Asia. Drought affects more than four million square kilometers every year, and is a significant factor limiting agricultural productivity. However, relatively little is known about how Coix lacryma-jobi L. responds to drought treatments. To obtain a detailed and comprehensive understanding of the mechanisms regulating the transcriptional responses of Coix lacryma-jobi L. to drought treatment, we employed high throughput short-read sequencing of cDNA prepared from polyadenylated RNA to explore global gene expression after a seven-day drought treatment. We generated a de novo assembled transcriptome comprising 65,480 unique sequences. Differential expression analysis based on RSEM-estimated transcript abundances identified 5,315 differentially expressed genes (DEGs) when comparing samples from plants following drought-treatment and from the appropriate controls. Among these, the transcripts for 3,460 genes were increased in abundance, whereas 1,855 were decreased. Real-time quantitative PCR for 5 transcripts confirmed the changes identified by RNA-Seq. The results provide a transcriptional overview of the changes in Coix lacryma-jobi L. in response to drought, and will be very useful for studying the function of associated genes and selection of molecular marker of Coix lacryma-jobi L in the future.Guidong MiaoYan QinJihua GuoQingxia ZhangYingying BaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256875 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guidong Miao Yan Qin Jihua Guo Qingxia Zhang Yingying Bao Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.
description	Coix lacryma-jobi L. is a very important economic crop widely cultivated in Southeast Asia. Drought affects more than four million square kilometers every year, and is a significant factor limiting agricultural productivity. However, relatively little is known about how Coix lacryma-jobi L. responds to drought treatments. To obtain a detailed and comprehensive understanding of the mechanisms regulating the transcriptional responses of Coix lacryma-jobi L. to drought treatment, we employed high throughput short-read sequencing of cDNA prepared from polyadenylated RNA to explore global gene expression after a seven-day drought treatment. We generated a de novo assembled transcriptome comprising 65,480 unique sequences. Differential expression analysis based on RSEM-estimated transcript abundances identified 5,315 differentially expressed genes (DEGs) when comparing samples from plants following drought-treatment and from the appropriate controls. Among these, the transcripts for 3,460 genes were increased in abundance, whereas 1,855 were decreased. Real-time quantitative PCR for 5 transcripts confirmed the changes identified by RNA-Seq. The results provide a transcriptional overview of the changes in Coix lacryma-jobi L. in response to drought, and will be very useful for studying the function of associated genes and selection of molecular marker of Coix lacryma-jobi L in the future.
format	article
author	Guidong Miao Yan Qin Jihua Guo Qingxia Zhang Yingying Bao
author_facet	Guidong Miao Yan Qin Jihua Guo Qingxia Zhang Yingying Bao
author_sort	Guidong Miao
title	Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.
title_short	Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.
title_full	Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.
title_fullStr	Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.
title_full_unstemmed	Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.
title_sort	transcriptome characterization and expression profile of coix lacryma-jobi l. in response to drought.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0750f287027b41d3aa43d6a24b2ee6c7
work_keys_str_mv	AT guidongmiao transcriptomecharacterizationandexpressionprofileofcoixlacrymajobilinresponsetodrought AT yanqin transcriptomecharacterizationandexpressionprofileofcoixlacrymajobilinresponsetodrought AT jihuaguo transcriptomecharacterizationandexpressionprofileofcoixlacrymajobilinresponsetodrought AT qingxiazhang transcriptomecharacterizationandexpressionprofileofcoixlacrymajobilinresponsetodrought AT yingyingbao transcriptomecharacterizationandexpressionprofileofcoixlacrymajobilinresponsetodrought
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Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought.

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