Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida

Abstract Francisella tularensis is a facultative intracellular Gram-negative bacterium that causes the zoonotic disease tularemia. We identified the transcription elongation factor GreA as a virulence factor in our previous study, but its role was not defined. Here, we investigate the effects of the...

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Autores principales:	Guolin Cui, Jun Wang, Xinyi Qi, Jingliang Su
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0755247e6f134dad9111b4449cb57bdc
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spelling	oai:doaj.org-article:0755247e6f134dad9111b4449cb57bdc2021-12-02T11:41:04ZTranscription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida10.1038/s41598-018-25271-52045-2322https://doaj.org/article/0755247e6f134dad9111b4449cb57bdc2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25271-5https://doaj.org/toc/2045-2322Abstract Francisella tularensis is a facultative intracellular Gram-negative bacterium that causes the zoonotic disease tularemia. We identified the transcription elongation factor GreA as a virulence factor in our previous study, but its role was not defined. Here, we investigate the effects of the inactivation of the greA gene, generating a greA mutant of F. tularensis subsp. novicida. Inactivation of greA impaired the bacterial invasion into and growth within host cells, and subsequently virulence in mouse infection model. A transcriptomic analysis (RNA-Seq) showed that the loss of GreA caused the differential expression of 196 bacterial genes, 77 of which were identified as virulence factors in previous studies. To confirm that GreA regulates the expression of virulence factors involved in cell invasion by Francisella, FTN_1186 (pepO) and FTN_1551 (ampD) gene mutants were generated. The ampD deletion mutant showed reduced invasiveness into host cells. These results strongly suggest that GreA plays an important role in the pathogenesis of Francisella by affecting the expression of virulence genes and provide new insights into the complex regulation of Francisella infection.Guolin CuiJun WangXinyi QiJingliang SuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guolin Cui Jun Wang Xinyi Qi Jingliang Su Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida
description	Abstract Francisella tularensis is a facultative intracellular Gram-negative bacterium that causes the zoonotic disease tularemia. We identified the transcription elongation factor GreA as a virulence factor in our previous study, but its role was not defined. Here, we investigate the effects of the inactivation of the greA gene, generating a greA mutant of F. tularensis subsp. novicida. Inactivation of greA impaired the bacterial invasion into and growth within host cells, and subsequently virulence in mouse infection model. A transcriptomic analysis (RNA-Seq) showed that the loss of GreA caused the differential expression of 196 bacterial genes, 77 of which were identified as virulence factors in previous studies. To confirm that GreA regulates the expression of virulence factors involved in cell invasion by Francisella, FTN_1186 (pepO) and FTN_1551 (ampD) gene mutants were generated. The ampD deletion mutant showed reduced invasiveness into host cells. These results strongly suggest that GreA plays an important role in the pathogenesis of Francisella by affecting the expression of virulence genes and provide new insights into the complex regulation of Francisella infection.
format	article
author	Guolin Cui Jun Wang Xinyi Qi Jingliang Su
author_facet	Guolin Cui Jun Wang Xinyi Qi Jingliang Su
author_sort	Guolin Cui
title	Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida
title_short	Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida
title_full	Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida
title_fullStr	Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida
title_full_unstemmed	Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida
title_sort	transcription elongation factor grea plays a key role in cellular invasion and virulence of francisella tularensis subsp. novicida
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0755247e6f134dad9111b4449cb57bdc
work_keys_str_mv	AT guolincui transcriptionelongationfactorgreaplaysakeyroleincellularinvasionandvirulenceoffrancisellatularensissubspnovicida AT junwang transcriptionelongationfactorgreaplaysakeyroleincellularinvasionandvirulenceoffrancisellatularensissubspnovicida AT xinyiqi transcriptionelongationfactorgreaplaysakeyroleincellularinvasionandvirulenceoffrancisellatularensissubspnovicida AT jingliangsu transcriptionelongationfactorgreaplaysakeyroleincellularinvasionandvirulenceoffrancisellatularensissubspnovicida
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Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida

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